Turn‐On Luminescent Probes for the Real‐Time Monitoring of Endogenous Hydroxyl Radicals in Living Cells

Abstract

The utilization of semiconductor quantum dots (QDs) as optical labels for biosensing and biorecognition has made substantial progress. However, the development of a suitable QD-based luminescent probe that is capable of detecting individual reactive oxygen species (ROS) represents a great challenge, mainly because the fluorescence of QDs is quenched by a wide variety of ROS. To overcome this limitation, a novel QD-based turn-on luminescent probe for the specific detection of (.) OH has been designed, and its application in monitoring the endogenous release of (.) OH species in living cells is demonstrated. Metal citrate complexes on the surfaces of the QDs can act as electron donors, injecting electrons into the LUMO of the QDs, while (.) OH can inject holes into the HOMO of the QDs. Accordingly, electron-hole pairs are produced, which could emit strong luminescence by electron-hole recombination. Importantly, this luminescent probe does not respond to other ROS.

MIL-53(Fe) MOF-mediated catalytic chemiluminescence for sensitive detection of glucose

“…Thus, various chemiluminescence (CL) systems have received considerable attention in the past years. To further improve the sensitivity of CL assays, various catalysts have been investigated, including metal nanoclusters [1], metallic nanoparticles (in particular noble metal nanoparticles) [5,6], metal oxide nanoparticles [7,8], carbon dots [9][10][11], layered double hydroxide (LDH) [4,12], quantum dots [13,14], and LDH-quantum dot nanocomposites [15].…”

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

“…Yang and co‐workers developed a fluorescent probe based on fluorescein, which was capable of imaging of OH • In RAW 264.7 cells. Similarly, Lu and co‐workers reported another fluorescence “turn‐on” probe for the imaging and real‐time monitoring of endogenous OH • in living HeLa cells.…”

Nanotechnologies for Reactive Oxygen Species“Turn-On” Detection

“…Consequently, the produced electron–hole pairs could emit strong luminescence through electron–hole recombination. This nanotechnology is demonstrated to have an application in detecting the endogenous release of • OH in living cells ( Zhou W. et al, 2016 ). Alternatively, Yu’s group fabricated a polyhedral-AuPd nanoparticle-based dual-mode cytosensor (PH-AuPd NPs, LOD information unavailable) with a “turn-on”-enabled signal for • OH sensing.…”