Turning mannitol-rich agricultural waste to poly(3-hydroxybutyrate) with Cobetia amphilecti fermentation and recovery with methyl levulinate as a green solvent

Purification and Characterization of the Enzyme Fucoidanase from Cobetia amphilecti Utilizing Fucoidan from Undaria pinnatifida

“…HN-25, therefore, was identified as Cobetia amphilecti , which is a novel species belonging to the genus Cobetia [ 51 ]. Studies have shown that glutaminase-free L-asparaginase [ 52 ], alkaline phosphatase/phosphodiesterase [ 53 ] and poly (3-hydroxybutyrate) (PHB) [ 54 ] were found in Cobetia amphilecti . The nucleolytic enzymes obtained from Cobetia amphilecti KMM 296 have also shown antibacterial activity [ 53 ].…”

Valorization of Cabbage Waste as a Feedstock for Microbial Polyhydroxyalkanoate Production: Optimizing Hydrolysis Conditions and Polyhydroxyalkanoate Production

“…13 Similarly, the bacterial strain Cobetia amphilecti could biosynthesize 1.53 g/L P(3HB) using 100 g/L celery waste extract as a substrate. 7 In addition, Kwan et al (2016) developed a Lactobacillus casei strain capable of producing 94.0 g/L lactic acid from mixed food waste. 19 The group achieved an 85% carbohydrate conversion rate through fungal hydrolysis, which led to the recovery of a hydrolysate that was rich in glucose and fructose.…”

“…Furthermore, P­(3HB) can be naturally biodegraded into organic carbon, contributing to the establishment of a sustainable carbon cycle. Therefore, significant effort has been made to develop sustainable and feasible microbial P­(3HB) production platforms. − However, current methods for microbial-based P­(3HB) production have challenges, such as low productivity and high costs. Although P­(3HB) productivity has been greatly improved through genetic engineering and process optimization, the raw materials still rely heavily on expensive refined sugars and oils, hindering further industrialization. , If needed, P­(3HB) can be produced from microorganisms using biomass resources, such as palm oil or plant sugar, as carbon sources.…”

“…For example, Zobellella denitrificans ZD1 could synthesize 0.45 g/L PHAs using orange peels as a carbon source after a water-based boiling process . Similarly, the bacterial strain Cobetia amphilecti could biosynthesize 1.53 g/L P­(3HB) using 100 g/L celery waste extract as a substrate . In addition, Kwan et al (2016) developed a Lactobacillus casei strain capable of producing 94.0 g/L lactic acid from mixed food waste .…”

Evaluation of the toxicity profiles of three families of solvents from biomass: levulinate, lactate and furfural derivatives

“…These solvents are obtained from sustainable sources, such as glucose, sucrose, or lignocellulose, by enzymatic processes, esterification, fermentation, 3 or catalysis, [4][5][6] making their use interesting when ecosustainability is a goal. All this has led to a wide and varying range of applications, highlighting their use as antimicrobial agents, 7 food additives, flavoring agents, 8,9 precursors for environmentally friendly plasticizers, 10 adjuvants in transdermal pharmaceuticals, 11 precursors for biologically active natural products, 12 precursors and intermediates in drug synthesis, 8,13 or even as a green solvent in different processes, [14][15][16] among others. In recent years, due to their associated green properties, they have even been used as components of other green solvents such as deep eutectic solvents, further broadening the scope of their applications.…”