Turnip mosaic virus, a virus for all seasons

Nellist, Charlotte F.; Ohshima, Kazusato; Ponz, F; Walsh, John A.

doi:10.1111/aab.12755

Cited by 25 publications

(15 citation statements)

References 139 publications

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“…Rod-type particles derived from tobacco mosaic virus (rigid) or potato virus X (flexuous) have received most of the attention in this context ( 2 ), but VLPs derived from turnip mosaic virus (TuMV), a flexuous rod-type potyvirus, have also shown an important potential for nanobiotechnological applications. Thus, chemically or genetically functionalized TuMV VLPs have been exploited for applications as diverse as antibody sensing, enzyme nano-immobilization, antimicrobials, or biofabrication ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines

Pazos-Castro

Margain²,

González-Klein³

et al. 2022

Front. Immunol.

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Virus-like particles (VLPs) have been gaining attention as potential platforms for delivery of cargos in nanomedicine. Although animal viruses are largely selected due to their immunostimulatory capacities, VLPs from plant viruses constitute a promising alternative to be considered. VLPs derived from Turnip mosaic virus (TuMV) have proven to present a tridimensional structure suited to display molecules of interest on their surface, making them interesting tools to be studied in theragnostic strategies. Here, we study their potential in the treatment of food allergy by genetically coupling TuMV-derived VLPs to Pru p 3, one of the most dominant allergens in Mediterranean climates. VLPs-Pru p 3 were generated by cloning a synthetic gene encoding the TuMV coat protein and Pru p 3, separated by a linker, into a transient high-expression vector, followed by agroinfiltration in Nicotiana benthamiana plants. The generated fusion protein self-assembled in planta to form the VLPs, which were purified by exclusion chromatography. Their elongated morphology was confirmed by electron microscopy and their size (~400 nm), and monodispersity was confirmed by dynamic light scattering. Initial in vitro characterization confirmed that they were able to induce proliferation of human immune cells. This proliferative capability was enhanced when coupled with the natural lipid ligand of Pru p 3. The resultant formulation, called VLP-Complex, was also able to be transported by intestinal epithelial cells, without affecting the monolayer integrity. In light of all these results, VLP-Complex was furtherly tested in a mouse model of food allergy. Sublingual administration of VLP-Complex could effectively reduce some serological markers associated with allergic responses in mice, such as anti-Pru p 3 sIgE and sIgG2a. Noteworthy, no associated macroscopic, nephritic, or hepatic toxicity was detected, as assessed by weight, blood urea nitrogen (BUN) and galectin-3 analyses, respectively. Our results highlight the standardized production of allergen-coated TuMV-VLPs in N. benthamiana plants. The resulting formula exerts notable immunomodulatory properties without the need for potentially hazardous adjuvants. Accordingly, no detectable toxicity associated to their administration was detected. As a result, we propose them as good candidates to be furtherly studied in the treatment of immune-based pathologies.

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Section: Introductionmentioning

confidence: 99%

Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines

Pazos-Castro

Margain²,

González-Klein³

et al. 2022

Front. Immunol.

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“…broccoli, cabbage, radish, and turnip) and ornamental plants across the world ( Ohshima, Yamaguchi, and Hirota et al. 2002 ; Walsh and Jenner 2002 ; Nellist et al. 2022 ) and is transmitted by aphids in a non-persistent manner.…”

Section: Introductionmentioning

confidence: 99%

The recombinogenic history of turnip mosaic potyvirus reveals its introduction to Japan in the 19th century

et al. 2022

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Characterizing the detailed spatial and temporal dynamics of plant pathogens can provide valuable information for crop protection strategies. However, the epidemiological characteristics and evolutionary trajectories of pathogens can differ markedly from one country to another. The most widespread and important virus of brassica vegetables, turnip mosaic potyvirus (TuMV), causes serious plant diseases in Japan. We collected 317 isolates of TuMV from Raphanus and Brassica plants throughout Japan over nearly five decades. Genomic sequences from these isolates were combined with published sequences. We identified a total of 88 independent recombination events in Japanese TuMV genomes, and found 82 recombination type patterns in Japan. We assessed the evolution of TuMV through space and time using whole and partial genome sequences of both nonrecombinants and recombinants. Our results suggest that TuMV was introduced into Japan after the country emerged from its isolationist policy (1639–1854) in the Edo period, then dispersed to other parts of Japan in the 20th century. The results of our analyses reveal the complex structure of the TuMV population in Japan and emphasize the importance of identifying recombination events in the genome. Our study also provides an example of surveying the epidemiology of a virus that is highly recombinogenic.

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“…We evaluated the host-age effect on the susceptibility to a collection of viruses from six different genera. After characterizing the host-age dependent susceptibility, we performed an evolution experiment using turnip mosaic virus (TuMV; species Turnip mosaic virus , genus Potyvirus , family Potyviridae ) [41]. TuMV has a 9.5 Kb positive single-stranded RNA genome that translates into a large polyprotein then processed by three viral-encoded proteases into ten mature products, plus a frameshift protein [42].…”

Section: Introductionmentioning

confidence: 99%

“…TuMV has a 9.5 Kb positive single-stranded RNA genome that translates into a large polyprotein then processed by three viral-encoded proteases into ten mature products, plus a frameshift protein [42]. TuMV infects over 300 plant species, though most belong to the Brassicaceae family [41][42][43]. We used two different TuMV isolates: an isolate naïve to arabidopsis (hereafter referred as AS) and an experimentally preadapted one (referred as DV).…”

Section: Introductionmentioning

confidence: 99%

Host age-dependent evolution of a plant RNA virus

Melero

González

Elena

2022

Preprint

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Viruses are obligate pathogens that entirely rely on their host resources to complete their infectious cycle. The availability of such resources depends upon external and internal factors, being host age one of the most relevant ones. The interplay between host age and virus evolution has not been thoroughly studied in plants. Here, we have used the Arabidopsis thaliana - turnip mosaic virus (TuMV) pathosystem to study plant-virus interactions and virus evolution at three different host ages: vegetative (juvenile), bolting (transition) and reproductive (mature) stages. After infecting plants with a naive and a well-adapted TuMV isolates, we observed that the older the host the faster and more severe the infections were. The same trend was observed for several other viruses. Thereafter, we experimentally evolved lineages of the naive and the well-adapted TuMV isolates in plants from each of the three developmental stages. All evolved viruses enhanced their infection phenotypes, being this increase more intense on viruses evolved in younger hosts. The genomic changes of the evolved viral lineages revealed mutation patterns that strongly depended on the founder viral isolate as well as on the age of the host wherein the lineages evolved.

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Turnip mosaic virus, a virus for all seasons

Cited by 25 publications

References 139 publications

Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines

Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines

The recombinogenic history of turnip mosaic potyvirus reveals its introduction to Japan in the 19th century

Host age-dependent evolution of a plant RNA virus

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