Tween-20 increases the immunoreactivity of apolipoprotein A-I in plasma

Kluge, Kathryn; Squillace, Steve J.; Mao, Simon J.T.; Kottke, Bruce A.

doi:10.1016/0005-2760(80)90136-8

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

1980

DOI: 10.1016/0005-2760(80)90136-8

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Tween-20 increases the immunoreactivity of apolipoprotein A-I in plasma

Kathryn Kluge

Steve J. Squillace

Simon J.T. Mao

et al.

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1995

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Cited by 35 publications

References 20 publications

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Monoclonal antibodies specific for apoproteins of lipophorins from the migratory locust

Schulz¹,

Horst²,

Amesz³

et al. 1987

Arch Insect Biochem Physiol

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Spleen lymphocytes from mice immunized with locust native low-density lipophorin A+ (LDLp) were fused with nonproducing myeloma cells, strain Sp 2/0. Hybridomas that were isolated from the fused cells produced antibodies specific for LDLp and the high-density lipophorin Ayellow (HDLp).Monoclonal strains were generated through cloning by limiting dilution from those hybridomas synthesizing antibodies specific for apolipophorins (apoLp)-I, -11, and -111 of LDLp. Additionally, a hybridoma strain that was obtained after fusion of lymphocytes from mice immunized with apoLp-Ill produced antibodies that bind to apoLp-Ill and native LDLp.Some features of LDLp and HDLp were studied using these antibodies. It could be demonstrated that apoLp-l and apoLp-ll are not immunochemically identical and are exposed in the native particle of both LDLp and HDLp. It was also shown that in both lipophorins apoLp-ll i s less exposed than apoLp-I, whereas in LDLp apoLp-Ill is mainly exposed; some apoLp-Ill could also be detected in HDLp.Tween-20, a nonionic detergent, appears to affect the binding of anti-apoLp-I, -11, and -111 to both LDLp and HDLp. The monoclonal antibodies specific for locust apolipophorins do not bind to the respective apoproteins of lipophorins from other insects.

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Monoclonal antibodies specific for apoproteins of lipophorins from the migratory locust

Schulz¹,

Horst²,

Amesz³

et al. 1987

Arch Insect Biochem Physiol

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show abstract

Association between apolipoproteins A-I and A-II as evidenced by immunochemical approach

Briones

Mao

1982

Experientia

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Antioxidant and prooxidant roles of copper in Tween 20-induced hemolysis of hamster and pig erythrocytes containing marginal vitamin E

Hamada

1995

Experientia

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The concentration-dependent effects of copper acting either as an antioxidant or as a prooxidant were examined in vitro using Tween 20-induced hemolysis. When cupric ion concentration was more than 10 microM, free copper(II) acted as a prooxidant; both extensive hemolysis and production of unknown thiobarbituric acid-reactive substance occurred in hamster and pig erythrocytes irrespective of vitamin E status. However, when cupric ion concentration was 2-4 microM in the incubation medium, copper showed a clear antioxidant activity, reducing both hemolysis and malondialdehyde production induced either by diluted peroxide-containing Tween 20 with ascorbic acid and sodium azide in vitamin E-deficient hamster erythrocytes, or by peroxide-containing Tween 20 in pig erythrocytes containing marginal amounts of vitamin E. Copper(II) is taken up by the erythrocytes, where copper(I)-complexes may contribute to the protection of cells with membrane vitamin E against oxidative radical attack.

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Tween-20 increases the immunoreactivity of apolipoprotein A-I in plasma

Cited by 35 publications

References 20 publications

Monoclonal antibodies specific for apoproteins of lipophorins from the migratory locust

Monoclonal antibodies specific for apoproteins of lipophorins from the migratory locust

Association between apolipoproteins A-I and A-II as evidenced by immunochemical approach

Antioxidant and prooxidant roles of copper in Tween 20-induced hemolysis of hamster and pig erythrocytes containing marginal vitamin E

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