Twenty-four-hour profiles of metabolic and stress hormones in sheep selected for a calm or nervous temperament

Rietema, Stacey; Blackberry, Margaret; Maloney, Shane K.; Martin, Graeme B.; Hawken, Penny; Blache, Dominique

doi:10.1016/j.domaniend.2015.05.005

Cited by 13 publications

(6 citation statements)

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“…It can be argued that hormonal changes and the influence of age contribute to observations of serum proteome profiles and this should be accounted for. For instance, studies in sheep have shown that there is a diurnal variation metabolic and stress-responsive hormones [117]. …”

Section: Discussionmentioning

confidence: 99%

Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

et al. 2016

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BackgroundUnlike humans, there is currently no publicly available reference mass spectrometry-based circulating acellular proteome data for sheep, limiting the analysis and interpretation of a range of physiological changes and disease states. The objective of this study was to develop a robust and comprehensive method to characterise the circulating acellular proteome in ovine serum.MethodsSerum samples from healthy sheep were subjected to shotgun proteomic analysis using nano liquid chromatography nano electrospray ionisation tandem mass spectrometry (nanoLC-nanoESI-MS/MS) on a quadrupole time-of-flight instrument (TripleTOF® 5600+, SCIEX). Proteins were identified using ProteinPilot™ (SCIEX) and Mascot (Matrix Science) software based on a minimum of two unmodified highly scoring unique peptides per protein at a false discovery rate (FDR) of 1% software by searching a subset of the Universal Protein Resource Knowledgebase (UniProtKB) database (http://www.uniprot.org). PeptideShaker (CompOmics, VIB-UGent) searches were used to validate protein identifications from ProteinPilot™ and Mascot.ResultsProteinPilot™ and Mascot identified 245 and 379 protein groups (IDs), respectively, and PeptideShaker validated 133 protein IDs from the entire dataset. Since Mascot software is considered the industry standard and identified the most proteins, these were analysed using the Protein ANalysis THrough Evolutionary Relationships (PANTHER) classification tool revealing the association of 349 genes with 127 protein pathway hits. These data are available via ProteomeXchange with identifier PXD004989.ConclusionsThese results demonstrated for the first time the feasibility of characterising the ovine circulating acellular proteome using nanoLC-nanoESI-MS/MS. This peptide spectral data contributes to a protein library that can be used to identify a wide range of proteins in ovine serum.Electronic supplementary materialThe online version of this article (doi:10.1186/s12953-017-0119-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

et al. 2016

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“…To quantify the frequency, duration, and amplitude of episodic events of LH in sheep, blood sampling every 10 to 20 min provides sufficient discrimination [22]. A similar sampling frequency is adequate to detect the episodic nature of other hormones that are secreted/or driven by the pituitary, such as prolactin and cortisol (See [23]). For the EUEs of body temperature in rats during the dark phase, the interval between ultradian events can vary from less than 50 min to longer than 4 h, with the most frequent intervals being 50 to 100 min (see figures 5 and 6 in [11]).…”

Section: Ultradian Rhythms: What Are They?mentioning

confidence: 99%

Episodic Ultradian Events—Ultradian Rhythms

Goh

Maloney

Mark

et al. 2019

Biology

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In the fast lane of chronobiology, ultradian events are short-term rhythms that have been observed since the beginning of modern biology and were quantified about a century ago. They are ubiquitous in all biological systems and found in all organisms, from unicellular organisms to mammals, and from single cells to complex biological functions in multicellular animals. Since these events are aperiodic and last for a few minutes to a few hours, they are better classified as episodic ultradian events (EUEs). Their origin is unclear. However, they could have a molecular basis and could be controlled by hormonal inputs—in vertebrates, they originate from the activity of the central nervous system. EUEs are receiving increasing attention but their aperiodic nature requires specific sampling and analytic tools. While longer scale rhythms are adaptations to predictable changes in the environment, in theory, EUEs could contribute to adaptation by preparing organisms and biological functions for unpredictability.

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“…For example, an aggregate score of locomotory behaviour and vocalisations, which measure reactivity towards a human in an approach/avoidance test and degree of agitation to confinement in an isolation box (Murphy, 1999;Putu, 1988), has been used to select for two divergent lines of 'nervous' and 'calm' sheep. Selection has occurred for more than 20 years and the lines have been used to investigate the impact of temperament on factors such as maternal behaviour (Murphy, 1999;Bickell et al, 2010Bickell et al, , 2011a, affiliative behaviour (Bickell et al, 2009(Bickell et al, , 2011b, sexual behaviour (Gelez et al, 2003;Chanvallon et al, 2010), reproductive biology (Blache and Bickell, 2010), metabolic impacts and feed efficiency (Rietema et al, 2015;Hawken et al, 2013;Henry et al, 2010) and to identify genetic markers of temperament (Ding et al, 2021;Qiu et al, 2016). The 'nervous-calm' trait was originally defined as "the fearfulness and reactivity of an animal in response to humans and novel environments."…”

Section: Introductionmentioning

confidence: 99%

Behavioural reactivity testing in sheep indicates the presence of multiple temperament traits

Atkinson

Doyle²,

Woodward³

et al. 2022

Behavioural Processes

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Twenty-four-hour profiles of metabolic and stress hormones in sheep selected for a calm or nervous temperament

Cited by 13 publications

References 65 publications

Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

Episodic Ultradian Events—Ultradian Rhythms

Behavioural reactivity testing in sheep indicates the presence of multiple temperament traits

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