Twenty-two novel microsatellite loci for lumpfish (Cyclopterus lumpus)

Skírnisdóttir, Sigurlaug; Ólafsdóttir, Guðbjörg Ásta; Olafsson, Kristinn; Jendrossek, Thomas; Lloyd, Hywel ap Dafydd; Helyar, Sarah J.; Pampoulie, Christophe; Daníelsdóttir, Anna Kristín; Kasper, Jacob M.

doi:10.1007/s12686-012-9762-y

Cited by 12 publications

(11 citation statements)

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“…Twenty‐two novel microsatellite DNA loci have been characterized for the species (Skirnisdottir et al . ), and its use in genetic stock identification has so far revealed three distinct genetic groups in the North Atlantic: Maine–Canada–Greenland; Iceland–Norway, and the Baltic Sea (Pampoulie et al . ) with little evidence of gene flow amongst these.…”

Section: Genetic Variation and Population Differentiation In Lumpfishmentioning

confidence: 99%

“…Little is known about the extent of population differentiation of lumpfish from different origins, and this is an area where studies are much needed in support of the development of hatchery stocks with desirable traits. Twenty-two novel microsatellite DNA loci have been characterized for the species (Skirnisdottir et al 2013), and its use in genetic stock identification has so far revealed three distinct genetic groups in the North Atlantic: Maine-Canada-Greenland; Iceland-Norway, and the Baltic Sea (Pampoulie et al 2014) with little evidence of gene flow amongst these. However, no information is available for most other Atlantic areas, and new genetic data from the English channel suggest that lumpfish there have low to moderate levels of genetic diversity (He = 0.53-0.61) and low genetic differentiation Pooley et al 2015).…”

Section: Genetic Variation and Population Differentiation In Lumpfishmentioning

confidence: 99%

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Use of lumpfish for sea‐lice control in salmon farming: challenges and opportunities

Powell

Treasurer²,

Pooley

et al. 2017

Reviews in Aquaculture

216

248

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Efficient sea-lice control remains one of the most important challenges for the salmon farming industry. The use of wrasse (Labridae) as cleaner fish offers an alternative to medicines for sea-lice control, but wrasse tend to become inactive in winter. Lumpfish (Cyclopterus lumpus) continue to feed on sea-lice at low temperatures, and commercial production has escalated from thousands of fish in 2010 to well over 30 million juveniles deployed in 2016. However, production still relies on the capture of wild broodstock, which may not be sustainable. To meet global industry needs, lumpfish production needs to increase to reach c. 50 million fish annually and this can only come from aquaculture. We review current production methods and the use of lumpfish in sea cages and identify some of the main challenges and bottlenecks facing lumpfish intensification. Our gap analysis indicates that the areas in most need of research include better control of maturation for year-round production; formulation of appropriate diets; artificial selection of elite lines with desirable traits; and development of vaccines for certified, disease-free juvenile production. The welfare of farmed lumpfish also needs to be better quantified, and more information is needed on optimal densities and tank design. Finally, the risk of farmed lumpfish escaping from net pens needs to be critically assessed, and we argue that it might be beneficial to recover cleaner fish from salmon cages after the production cycle, perhaps using them as broodstock, for export to the Asian food markets or for the production of animal feeds.

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Section: Genetic Variation and Population Differentiation In Lumpfishmentioning

confidence: 99%

Section: Genetic Variation and Population Differentiation In Lumpfishmentioning

confidence: 99%

Use of lumpfish for sea‐lice control in salmon farming: challenges and opportunities

Powell

Treasurer²,

Pooley

et al. 2017

Reviews in Aquaculture

216

248

View full text Add to dashboard Cite

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“…Genomic DNA was extracted using a 10% Chelex (Bio-Rad; www.bio-rad.com) solution, as described in Walsh et al (1991). Eleven microsatellite loci (Clu-02, Clu-05, Clu-10, Clu-12, Clu-26, Clu-29, Clu-33, Clu-37, Clu-38, Clu-40 and Clu-45;Skirnisdóttir et al, 2013) were amplified using a Qiagen Multiplex PCR kit (www.qiagen.com) following the manufacturer's instructions. The temperature profile used for PCR was: initial denaturation at 95 ∘ C for 15 min., followed by 30 cycles at 94 ∘ C for 30 s, 57 ∘ C for 90 s and 70 s at 72 ∘ C. The final extension was conducted at 60 ∘ C for 30 min.…”

Section: O L E C U L a R A Na Ly S E Smentioning

confidence: 99%

Genetic structure of West Greenland populations of lumpfish Cyclopterus lumpus

García-Mayoral

Olsen

Hedeholm

et al. 2016

Journal of Fish Biology

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In this study, 11 microsatellite markers were used to determine the structure of West Greenlandic lumpfish Cyclopterus lumpus populations across six spawning locations spanning >1500 km and compared with neighbouring populations in Canada and Iceland. To evaluate whether data allow for identification of origin of C. lumpus in Greenlandic waters, genetic assignment analysis was performed for 86 C. lumpus sampled on a feeding migration. Significant structuring with isolation by distance was observed in the West Greenland samples and two major subpopulations, north and south, were suggested. Based on F values, closer relationships were observed between Greenland and Canada, than Greenland and Iceland. Surprisingly, the North Greenland population showed more similarities with Canadian samples, than did the geographically closer south-west Greenland population. Origin could be assigned for a high proportion of non-spawning fish and demonstrated a marked east-west spatial separation of fish of Greenlandic and Icelandic genotypes.

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“…Species with large north-south distributions such as juvenile turbot and Atlantic cod have been shown to display differences in terms of T opt SGR between latitudinal groups, indicating a genetic difference between populations which cannot be overcome during an acclimatization process (Imsland et al 2000;Pörtner et al 2001). This may very well be a pronounced effect in lumpfish culture as well, as this is a specie with a large geographic distribution and large size differences between populations (Davenport 1985), and large observed polymorphism in loci between populations (Skirnisdottir et al 2013). It is however uncertain to what extent genes affect fitness.…”

Section: Size-related Growth and Optimum Temperatures For Growthmentioning

confidence: 99%

“…The same study furthermore suggested that the energy allocation and utilization of a specie can vary according to differences in the environment. Lumpfish have been found to display high polymorphism in genetic loci between Icelandic and Canadian populations (Skirnisdottir et al 2013). Due to the large geographic distribution one may hence be able to exploit differences in feed utilization in culture of juvenile lumpfish for commercial production as well, if such genetic effects of growth exists in lumpfish.…”

Section: Effect Of Temperature On Feed Related Parametersmentioning

confidence: 99%