Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in <i>Arabidopsis thaliana</i>

Wang, Kun; Guo, Qiang; Froehlich, John E.; Hersh, Hope Lynn; Zienkiewicz, Agnieszka; Howe, Gregg A.; Benning, Christoph

doi:10.1105/tpc.18.00250

Cited by 110 publications

(82 citation statements)

References 72 publications

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“…Our finding that HAI1-AHL10 signaling regulated JA metabolism (AOS and AOC1) and JA responsive (JR2, VSP2) genes suggests that HAI1 also has a feedback effect on JA. This result is also consistent with other reports of cross-regulation between JA and ABA metabolism (57). In our previous characterization of HAI1, we noted that hai1-2 had altered expression of many defense-related genes and concluded that HAI1 could be involved in tradeoffs between pathogen defense and drought response (15).…”

Section: The Growth Assays Gene Expression Analysis and Localizationsupporting

confidence: 92%

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

Wong

Bhaskara²,

Teng³

et al. 2018

Preprint

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The Clade A protein phosphatase 2C Highly ABA-Induced 1 (HAI1) plays an important role in stress signaling yet little information is available on HAI1-regulated phosphoproteins.Quantitative phosphoproteomics identified phosphopeptides of increased abundance in hai1-2 in unstressed plants and in plants exposed to low water potential (drought) stress. The identity and localization of the phosphoproteins as well as enrichment of specific phosphorylation motifs indicated that these phosphorylation sites may be regulated directly by HAI1 or by HAI1regulated kinases including Mitogen-Activated Protein Kinases (MPKs), Sucrose-non fermenting Related Kinase 2 (SnRK2s) or Casein Kinases. One of the phosphosites putatively regulated by HAI1 was S313/S314 of AT Hook-Like10 (AHL10), a DNA binding protein of unclear function.HAI1 could directly dephosphorylate AHL10 in vitro and the level of HAI1 expression affected the abundance of phosphorylated AHL10 in vivo. AHL10 S314 phosphorylation was critical for restriction of plant growth under low water potential stress and for regulation of Jasmonic Acid and Auxin-related gene expression as well as expression of developmental regulators including Shootmeristemless (STM). These genes were also mis-regulated in hai1-2. AHL10 S314 phosphorylation was required for AHL10 complexes to form foci within the nucleoplasm, suggesting that S314 phosphorylation may control AHL10 association with the nuclear matrix or with other transcriptional regulators. These data identify a set of HAI1-affected phosphorylation sites, show that HAI1-regulated phosphorylation of AHL10 S314 controls AHL10 function and localization and also indicate that HAI1-AHL10 signaling coordinates growth with stress and defense responses.

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Section: The Growth Assays Gene Expression Analysis and Localizationsupporting

confidence: 92%

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

Wong

Bhaskara²,

Teng³

et al. 2018

Preprint

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“…However, Thompson et al . () suggested that possible disruptions of JA signaling might be the cause of the observed developmental changes in the rbl10 mutants and there is an increasing body of work demonstrating a relationship between chloroplast glycerolipid metabolism and JA biosynthesis (Lin et al ., ; Wang et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…Phytohormone analysis was performed using 4‐week‐old WT, rbl10‐ 1, and rbl10‐ 2 plant leaves wounded with a hemostat and extracts prepared and quantified essentially as described in Wang et al ., ; (Wang et al ., ). Time points were collected as follows: before wounding, 20 min after wounding, and 60 min after wounding.…”

Section: Methodsmentioning

confidence: 99%

A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana

et al. 2019

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Summary The thylakoid membranes of the chloroplast harbor the photosynthetic machinery that converts light into chemical energy. Chloroplast membranes are unique in their lipid makeup, which is dominated by the galactolipids mono‐ and digalactosyldiacylglycerol (MGDG and DGDG). The most abundant galactolipid, MGDG, is assembled through both plastid and endoplasmic reticulum (ER) pathways in Arabidopsis, resulting in distinguishable molecular lipid species. Phosphatidic acid (PA) is the first glycerolipid formed by the plastid galactolipid biosynthetic pathway. It is converted to substrate diacylglycerol (DAG) for MGDG Synthase (MGD1) which adds to it a galactose from UDP‐Gal. The enzymatic reactions yielding these galactolipids have been well established. However, auxiliary or regulatory factors are largely unknown. We identified a predicted rhomboid‐like protease 10 (RBL10), located in plastids of Arabidopsis thaliana, that affects galactolipid biosynthesis likely through intramembrane proteolysis. Plants with T‐DNA disruptions in RBL10 have greatly decreased 16:3 (acyl carbons:double bonds) and increased 18:3 acyl chain abundance in MGDG of leaves. Additionally, rbl10‐1 mutants show reduced [14C]–acetate incorporation into MGDG during pulse−chase labeling, indicating a reduced flux through the plastid galactolipid biosynthesis pathway. While plastid MGDG biosynthesis is blocked in rbl10‐1 mutants, they are capable of synthesizing PA, as well as producing normal amounts of MGDG by compensating with ER‐derived lipid precursors. These findings link this predicted protease to the utilization of PA for plastid galactolipid biosynthesis potentially revealing a regulatory mechanism in chloroplasts.

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“…For example, JAM1 regulates both ABA and JA responses in Arabidopsis (Li et al ., ; Nakata et al ., ). ABA could promote the expression of PLIP2 and PLIP3, which might be involved in initiating JA biosynthesis (Wang et al ., ). In this study, we reveal two mechanisms underlying the crosstalk between JA and ABA: JAZ proteins can physically interact with ABI5 and repress its activity; ABA can induce JA biosynthesis to promote the degradation of JAZ proteins.…”

Section: Discussionmentioning

confidence: 97%

JAZ proteins modulate seed germination through interaction with ABI5 in bread wheat and Arabidopsis

et al. 2019

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Summary Appropriate regulation of crop seed germination is of significance for agriculture production. In this study, we show that TaJAZ1, most closely related to Arabidopsis JAZ3, negatively modulates abscisic acid (ABA)‐inhibited seed germination and ABA‐responsive gene expression in bread wheat. Biochemical interaction assays demonstrate that the C‐terminal part containing the Jas domain of TaJAZ1 physically interacts with TaABI5. Similarly, Arabidopsis jasmonate‐ZIM domain (JAZ) proteins also negatively modulate ABA responses. Further we find that a subset of JAZ proteins could interact with ABI5 using the luciferase complementation imaging assays. Choosing JAZ3 as a representative, we demonstrate that JAZ3 interacts with ABI5 in vivo and represses the transcriptional activation activity of ABI5. ABA application could abolish the enrichment of JAZ proteins in the ABA‐responsive gene promoter. Furthermore, we find that ABA application could induce the expression of jasmonate (JA) biosynthetic genes and then increase the JA concentrations partially dependent on the function of ABI5, consequently leading to the degradation of JAZ proteins. This study sheds new light on the crosstalk between JA and ABA in modulating seed germination in bread wheat and Arabidopsis.

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Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis in Arabidopsis thaliana

Cited by 110 publications

References 72 publications

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana

JAZ proteins modulate seed germination through interaction with ABI5 in bread wheat and Arabidopsis

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