Two C3H Type Zinc Finger Protein Genes, CpCZF1 and CpCZF2, from Chimonanthus praecox Affect Stamen Development in Arabidopsis

Liu, Hua‐Min; Huang, Ruxun; Ma, Jing; Sui, Shunzhao; Guo, Yanjun; Liu, Daofeng; Li, Zhineng; Lin, Yan; Li, Mingyang

doi:10.3390/genes8080199

Cited by 24 publications

(23 citation statements)

References 59 publications

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“…Subcellular Localization, Semi-Quantitative Reverse Transcription PCR and Quantitative Real-Time PCR (qRT-PCR) Subcellular localization of EjPI was detected using the modified pCAMBIA 1300 vector (Liu et al, 2017) and Agrobacterium- mediated transient transformation in Nicotiana benthamiana leaves. Then, fluorescence signals of green fluorescent protein (GFP) were observed via fluorescent microscopy using an Observer DP80 (Olympus, Japan).…”

Section: Sequence Alignments and Phylogenetic Analysismentioning

confidence: 99%

Expression Pattern and Functional Characterization of PISTILLATA Ortholog Associated With the Formation of Petaloid Sepals in Double-Flower Eriobotrya japonica (Rosaceae)

et al. 2020

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Section: Sequence Alignments and Phylogenetic Analysismentioning

confidence: 99%

Expression Pattern and Functional Characterization of PISTILLATA Ortholog Associated With the Formation of Petaloid Sepals in Double-Flower Eriobotrya japonica (Rosaceae)

et al. 2020

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“…Wintersweet AGAMOUS-like6 ( CpAGL6 ) gene was reported to promote flowering through inhibition of FLOWERING LOCUS C ( FLC ) and activation of APETALA1 ( AP1 ) and FLOWERING LOCUS T ( FT ) and cause abnormal stamen and carpel development in later developing flowers [4]. CpCZF1 and CpCZF2 , two C3H type zinc finger protein genes of wintersweet, were revealed to affect stamen development and caused the formation of abnormal flowers in transgenic Arabidopsis plants [5]. Additionally, for ornamental plants and cut flowers, the research on senescence is always one of the hot issues, which is closely related to its commercial value and market competitiveness [6].…”

Section: Introductionmentioning

confidence: 99%

CpWRKY71, a WRKY Transcription Factor Gene of Wintersweet (Chimonanthus praecox), Promotes Flowering and Leaf Senescence in Arabidopsis

Huang¹,

Liu²,

Huang³

et al. 2019

IJMS

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The WRKY transcription factors are one of the most important plant-specific transcription factors and play vital roles in various biological processes. However, the functions of WRKY genes in wintersweet (Chimonanthus praecox) are still unknown. In this report, a group IIc WRKY gene, CpWRKY71, was isolated from wintersweet. CpWRKY71 was localized to the nucleus and possessed transcriptional activation activity. qRT-PCR (quantitative real-time PCR) analysis showed that CpWRKY71 was expressed in all tissues tested, with higher expression in flowers and senescing leaves. During the flower development, the highest expression was detected in the early-withering stage, an obvious expression of CpWRKY71 was also observed in the flower primordia differentiation and the bloom stage. Meanwhile, the expression of CpWRKY71 was influenced by various abiotic stress and hormone treatments. The expression patterns of the CpWRKY71 gene were further confirmed in CpWRKY71pro:GUS (β-glucuronidase) plants. Heterologous overexpression of CpWRKY71 in Arabidopsis caused early flowering. Consistent with the early flowering phenotype, the expression of floral pathway integrators and floral meristem identity (FMI) genes were significantly up-regulated in transgenic plants. In addition, we also observed that the transgenic plants of CpWRKY71 exhibited precocious leaf senescence. In conclusion, our results suggested that CpWRKY71 may be involved in the regulation of flowering and leaf senescence in Arabidopsis. Our study provides a foundation for further characterization of CpWRKY genes function in wintersweet, and also enrich our knowledge of molecular mechanism about flowering and senescence in wintersweet.

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“…A phylogenetic tree was constructed using MEGA 5.0 software (Kumar et al , Tamura et al ) and the method described by Jing et al (). Subcellular localization of EjAG was detected using the modified pCAMBIA 1300 vector ( 35S promoter, Cter‐ GFP ; Liu et al ) and Agrobacterium ‐mediated transient transformation of Nicotiana benthamiana leaves. Then, green fluorescent protein (GFP) signals were observed via fluorescent microscopy using an Observer DP80 (Olympus).…”

Section: Methodsmentioning

confidence: 99%

Homeotic transformation from stamen to petal in Eriobotrya japonica is associated with hormone signal transduction and reduction of the transcriptional activity of EjAG

Jing

Chen

Xia

et al. 2019

Physiologia Plantarum

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Double-flower loquat (Eriobotrya japonica) is a new germplasm with homeotic transformation of stamen into petal in whorl 3. However, little information is available on the molecular mechanism of this transformation. Herein, we analyzed the transcriptome, candidate genes and endogenous hormones to investigate the mechanisms underlying this homeotic transformation. Some transcription factors, such as MADS-box, TCP and MYB, were significantly differentially expressed. Importantly, we confirmed that one of these (DN39625_c0_g1), which encoded a C-class floral homeotic protein referred to as AGAMOUS ortholog (EjAG), was significantly downregulated. Subcellular localization of EjAG was found to be in the nucleus. Ectopic expression of EjAG rescued the development of stamens and carpels from the double-flower phenotype in an Arabidopsis ag mutant, suggesting that EjAG expression is associated with double-flower formation. Meanwhile, enrichment analyses showed that the differentially expressed genes (DEGs) were mainly involved in the metabolic pathways of hormone signal transduction. The DEGs of auxin, gibberellin A (GA) and cytokinin signaling pathways were mainly upregulated. However, the DEGs of abscisic acid (ABA) and the ethylene signaling pathway were mainly downregulated. Accordingly, the concentrations of indoleacetic acid, kinetin and GA 3 were high at the petaloid stamen stage, but the ABA concentration remained low. The identified genes and pathways provide abundant sequence resources for studying the mechanisms underlying the homeotic transformation in loquat and other Rosaceae species.

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Two C3H Type Zinc Finger Protein Genes, CpCZF1 and CpCZF2, from Chimonanthus praecox Affect Stamen Development in Arabidopsis

Cited by 24 publications

References 59 publications

Expression Pattern and Functional Characterization of PISTILLATA Ortholog Associated With the Formation of Petaloid Sepals in Double-Flower Eriobotrya japonica (Rosaceae)

Expression Pattern and Functional Characterization of PISTILLATA Ortholog Associated With the Formation of Petaloid Sepals in Double-Flower Eriobotrya japonica (Rosaceae)

CpWRKY71, a WRKY Transcription Factor Gene of Wintersweet (Chimonanthus praecox), Promotes Flowering and Leaf Senescence in Arabidopsis

Homeotic transformation from stamen to petal in Eriobotrya japonica is associated with hormone signal transduction and reduction of the transcriptional activity of EjAG

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