Two calcium‐binding chaperones from the fat body of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) involved in diapause

Abstract

Molecular chaperones are crucial for the correct folding of newly synthesized polypeptides, in particular, under stress conditions. Various studies have revealed the involvement of molecular chaperones, such as heat shock proteins, in diapause maintenance and starvation; however, the role of other chaperones in diapause and starvation relatively is unknown. In the current study, we identified two lectintype chaperones with calcium affinity, a calreticulin (LdCrT) and a calnexin (LdCnX), that were present in the fat body of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) during diapause. Both proteins possessed an N-globular domain, a P-arm domain, and a highly charged C-terminal domain, while an additional transmembrane domain was present in LdCnX. Phylogenetic analysis revealed distinction at the order level. Both genes were expressed in multiple tissues in larval and adult stages, and constitutively throughout development, though a starvation response was detected only for LdCrT. In females, diapause-related expression analysis in the whole body revealed an upregulation of both genes by post-diapause, but a downregulation by diapause only for LdCrT. By contrast, males revealed no alteration in their

Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L.

“…In drosophilids, suppressing the expression of Hsp23 andHsp70 by RNAi did not alter the commitment to enter diapause or the duration of diapause, but it is critical for cold survival during diapause in insects ( Rinehart et al, 2007 ). Furthermore, the adipose body-specific expression of two calcium-binding chaperones (LDCRT and LDCNX) in L. decemlineata during diapause revealed the same expression pattern in relation to diapause; there was no alteration in females and downregulation in males post-diapause ( Doğan et al, 2021 ).…”

What You Eat Matters: Nutrient Inputs Alter the Metabolism and Neuropeptide Expression in Egyptian Cotton Leaf Worm, Spodoptera littoralis (Lepidoptera: Noctuidae)

“…Specifically, the decreases were around 14 and 12% in third and fifth instar larvae, respectively. Various studies in mammals and insects revealed the involvement of dietary Ca 2+ and specific Ca 2+ signaling genes in lipid metabolism, leading to lipolysis ( Allen and Beck, 1986 ; Shi et al, 2001 ; Zemel, 2002 ; Jacqmain et al, 2003 ; Arruda and Hotamisligil, 2015 ; Maus et al, 2017 ; Doğan et al, 2021a , b ; Toprak et al, 2021 ). In Drosophila, elevated cytosolic Ca 2+ levels lead to activation of lipolysis ( Subramanian et al, 2013a , b ; Baumbach et al, 2014a , b ; Bi et al, 2014 ).…”

“…HFD, HSD, a calcium-rich diet (CRD), and a plant-based diet (PBD) were supplied throughout the larval development and compared to a standard artificial bean diet. The CRD was included as studies have indicated the involvement of Ca 2+ signaling molecules, cellular, and dietary Ca 2+ in lipid and carbohydrate metabolism, body weight regulation, obesity, and diabetes in insects and mammals ( Zemel et al, 2000 ; Shi et al, 2001 ; Zemel, 2002 ; Jacqmain et al, 2003 ; Baumbach et al, 2014a , b ; Arruda and Hotamisligil, 2015 ; Maus et al, 2017 ; Alomaim et al, 2019 ; Doğan et al, 2021a , b ; Toprak et al, 2021 ). The PBD (lettuce leaves) was used as lettuce is a natural host for S .…”

The Colorado potato beetle gene expression atlas

“…Recent studies have focused on finding alleles linked with resistance 18,19 and gene expression linked with resistance 20 . The CPB has also been used as a model to understand insect development and physiology [21][22][23] , insect diapause [24][25][26][27] , and more recently insect lipid metabolism and calcium signaling 28,29 as well as behavior 30 .…”