Two cases of Plasmodium ovale malaria from Central Thailand

Fc, Cadigan; Rs, Desowitz

doi:10.1016/0035-9203(69)90194-1

Cited by 13 publications

(9 citation statements)

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“…Cases of P. ovale malaria have been reported in most countries in the Mekong sub-region, except in Cambodia. [6][7][8][9][10][11][12][13][14][15][16][17][18] Our study is therefore the first description of P. ovale in this country. The prevalence of P. ovale was nearly 4% in the 102 DNA samples examined here.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmodium ovale was long thought to be absent from southeast Asia. The first microscopic identifications of P. ovale were reported in the 1970s in countries surrounding Cambodia, [6][7][8][9] including cases along the eastern and northwestern borders of Cambodia. [10][11][12] Molecular diagnosis methods have recently led to the identification of even more P. malariae and P. ovale infections in the southeast Asian region.…”

Section: Introductionmentioning

confidence: 99%

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Large Sequence Heterogeneity of the Small Subunit Ribosomal Rna Gene of Plasmodium Ovale in Cambodia

Incardona¹,

Chy²,

Lim³

et al. 2005

The American Journal of Tropical Medicine and Hygiene

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Plasmodium ovale malaria has been reported in various countries in southeast Asia, but never in Cambodia. Using a species-specific polymerase chain reaction (PCR) targeting the small subunit (SSU) ribosomal RNA (rRNA) gene, we detected P. ovale in nearly 4% of the inhabitants of a northeastern Cambodian village. Plasmodium ovale was associated with at least one other Plasmodium species, and two quadruple infections were detected. The diagnosis was confirmed by microscopy and by SSU rRNA PCR product sequencing. The sequences shared 96-99% identity with published sequences, and displayed a substantial heterogeneity with 2-4 different haplotypes per sample. Nine distinct SSU rRNA haplotypes were identified, including seven novel variants. Phylogenetic analysis showed two major genetic clusters, suggesting amplification of two distinct gene sets and/or P. ovale variants from each sample. Our data indicate that P. ovale was overlooked in Cambodia until now, and call for the implementation of larger prevalence surveys and accurate diagnosis methods in this country.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Large Sequence Heterogeneity of the Small Subunit Ribosomal Rna Gene of Plasmodium Ovale in Cambodia

Incardona¹,

Chy²,

Lim³

et al. 2005

The American Journal of Tropical Medicine and Hygiene

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“…Similarly, the available official data from Myanmar recorded a low prevalence (0.04%) of P. malariae (Department of Health et al 1996). P. ovale is virtually absent from official reports from both countries; only two P. ovale infections were recorded in the past two decades in Thailand (Cadigan & Desowitz 1969) cells, were scanned for 2-3 min. Then, additional thin and thick smears were obtained from malaria-positive patients.…”

Section: Sample Collectionmentioning

confidence: 99%

High prevalence of Plasmodium malariae and Plasmodium ovale in malaria patients along the Thai‐Myanmar border, as revealed by acridine orange staining and PCR‐based diagnoses

Zhou

Liu

Wongsrichanalai

et al. 1998

Tropical Med Int Health

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SummaryThe prevalence of the four human malaria parasites was investigated among malaria patients at northern, central and southern towns in Thailand along the border with Myanmar between September 1995 and May 1996. Thin smears obtained from 548 Thai and Burmese patients were reviewed by an acridine orange staining method, and many mixed infections with two to four species, including P. malariae and P. ovale, were detected. These diagnostic results were compared with those by two PCR-based diagnoses, microtitre plate hybridization (MPH) and a nested PCR method, both of which targets the same, species-specific regions in the 18S rRNA genes. In both PCR diagnoses, many P. malariae and P. ovale infections were also detected. Detection sensitivity of P. malariae infection was higher in nested PCR than MPH, and a total prevalence of P. malariae infection estimated by nested PCR reached 24.3% (133/548). In 16 of them, the size of PCR products amplified by the P. malariae-specific primer was about 20-bp shorter than the expected size of 115-bp. Four of 16 possessed two different bands with normal and shorter sizes, suggesting that P. malariae isolates may be separated into two types, and that those with shorter products may be new variant form (s) with a nucleotide deletion in the target region. On the other hand, 21 P. ovale infections (3.8%) were detected by nested PCR, but four of them were MPH-negative because of the sequence variation at the probe region. These results indicated that the prevalence of P. malariae and P. ovale along the ThaiMyanmar border may be substantially higher than previously reported.keywords Plasmodium malariae, Plasmodium ovale, Thailand, Myanmar, acridine orange staining, PCR diagnosis correspondence Dr F. Kawamoto,

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“…However, it appears to be more widely distributed, having been reported in the Middle East, the Indian Subcontinent and various parts of Southeast Asia [8]–[11]. P. ovale has not been yet reported in South America.…”

Section: Introductionmentioning

confidence: 97%

Chimpanzee Malaria Parasites Related to Plasmodium ovale in Africa

et al. 2009

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Since the 1970's, the diversity of Plasmodium parasites in African great apes has been neglected. Surprisingly, P. reichenowi, a chimpanzee parasite, is the only such parasite to have been molecularly characterized. This parasite is closely phylogenetically related to P. falciparum, the principal cause of the greatest malaria burden in humans. Studies of malaria parasites from anthropoid primates may provide relevant phylogenetic information, improving our understanding of the origin and evolutionary history of human malaria species. In this study, we screened 130 DNA samples from chimpanzees (Pan troglodytes) and gorillas (Gorilla gorilla) from Cameroon for Plasmodium infection, using cytochrome b molecular tools. Two chimpanzees from the subspecies Pan t. troglodytes presented single infections with Plasmodium strains molecularly related to the human malaria parasite P. ovale. These chimpanzee parasites and 13 human strains of P. ovale originated from a various sites in Africa and Asia were characterized using cytochrome b and cytochrome c oxidase 1 mitochondrial partial genes and nuclear ldh partial gene. Consistent with previous findings, two genetically distinct types of P. ovale, classical and variant, were observed in the human population from a variety of geographical locations. One chimpanzee Plasmodium strain was genetically identical, on all three markers tested, to variant P. ovale type. The other chimpanzee Plasmodium strain was different from P. ovale strains isolated from humans. This study provides the first evidence of possibility of natural cross-species exchange of P. ovale between humans and chimpanzees of the subspecies Pan t. troglodytes.

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Two cases of Plasmodium ovale malaria from Central Thailand

Cited by 13 publications

References 1 publication

Large Sequence Heterogeneity of the Small Subunit Ribosomal Rna Gene of Plasmodium Ovale in Cambodia

Large Sequence Heterogeneity of the Small Subunit Ribosomal Rna Gene of Plasmodium Ovale in Cambodia

High prevalence of Plasmodium malariae and Plasmodium ovale in malaria patients along the Thai‐Myanmar border, as revealed by acridine orange staining and PCR‐based diagnoses

Chimpanzee Malaria Parasites Related to Plasmodium ovale in Africa

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