Two Cell Wall β-<scp>d</scp>-Glucans from<i>Neurospora crassa</i>

Hiura, Nozomi; Nakajima, Tasuku; Matsuda, Kazuo

doi:10.1080/00021369.1983.10866078

Cited by 7 publications

(6 citation statements)

References 4 publications

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“…Mycelia were harvested by centrifugation (7500 g, 30 min) and washed four times with distilled water to remove loosely attached cell-surface polysaccharides, and the cell walls then extracted and fractionated by a modification of the method of Hiura et al [21,42]. The washed cells were homogenized for 10 min in a Braun MSK homogenizer with 0.25-0.30 mm diameter Ballotini beads, and the homogenate autoclaved for 60 min at 121 mC.…”

Section: Fractionation Of a Persicinum Cell Wallsmentioning

confidence: 99%

Purification and characterization of an extracellular (1 → 6)-β-glucanase from the filamentous fungus Acremonium persicinum

Pitson

Seviour

McDougall

et al. 1996

Biochemical Journal

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An endo-(1 --> 6)-beta-glucanase has been isolated from the culture filtrates of the filamentous fungus Acremonium persicinum and purified by (NH4)2SO4 precipitation followed by anion-exchange and gel-filtration chromatography. SDS/PAGE of the purified enzyme gave a single band with an apparent molecular mass of 42.7 kDa. The enzyme is a non-glycosylated, monomeric protein with a pI of 4.9 and pH optimum of 5.0. It hydrolysed (1 --> 6)-beta-glucans (pustulan and lutean), initially yielding a series of (1 --> 6)-beta-linked oligoglucosides, consistent with endo-hydrolytic action. Final hydrolysis products from these substrates were gentiobiose and gentiotriose, with all products released as beta-anomers, indicating that the enzyme acts with retention of configuration. The purified enzyme also hydrolysed Eisenia bicyclis laminarin, liberating glucose, gentiobiose, and a range of larger oligoglucosides, through the apparent bydrolysis of (1 --> 6)-beta- and some (1 --> 3)-beta-linkages in this substrate. K(m) values for pustulan, lutean and laminarin were 1.28, 1.38, and 1.67 mg/ml respectively. The enzyme was inhibited by N-acetylimidazole, N-bromosuccinimide, dicyclohexylcarbodi-imide, Woodward's Reagent K, 2-hydroxy-5-nitrobenzyl bromide, KMnO4 and some metal ions, whereas D-glucono-1,5-lactone and EDTA had no effect.

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Section: Fractionation Of a Persicinum Cell Wallsmentioning

confidence: 99%

Purification and characterization of an extracellular (1 → 6)-β-glucanase from the filamentous fungus Acremonium persicinum

Pitson

Seviour

McDougall

et al. 1996

Biochemical Journal

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“…8 ) It is well known that fungal cell walls contain P-D-glucan with different proportions of 1,3-and 1,6-linkages, For Neurospora crassa, the heterogeneity of P-D-glucan was also proved to be the case by the isolation of two kinds of P-l ,3-D-glucans from the cell wal1. 9 ) It is still not certain, however, whether or not such heterogeneity is due to the modification of the cell wall P-D-glucan by the lytic enzymes, since the foregoing results are contradict each other.…”

mentioning

confidence: 66%

Change of the Structure of Cell Wallβ-l,3-d-Glucan with the Growth ofNeurospora crassaCells

Hiura¹,

Honjyo²,

Nakajima³

et al. 1984

Agricultural and Biological Chemistry

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“…1 -4 ) For example, the colonial morphology of the sorbose-grown wild-type strain was found to be related to either an increase in the level of fraction I (glucan) or a decrease in fraction' III 3 glucan fraction~. 7,[18][19][20][21] Although there have also been a few reports on some kinds of morphological mutants of R. so/ani, the relationship between the cell-wall components and the morphological chnage in R. so/ani is not as clear as in the case of Neurospora. The ropy-type mutant (spreading colonial) of R. so/ani was, however, newly isolated and its cell-wall component was examined as to the relationship mentioned above.…”

Section: Discussionmentioning

confidence: 99%