Two-Color Flow Cytometric Analysis of Preterm and Term Newborn Lymphocytes

Juretić, Emilija; Užarević, Branka; Petrovečki, Mladen; António, José

doi:10.1016/s0171-2985(00)80101-1

Cited by 31 publications

(31 citation statements)

References 33 publications

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“…These factors can influence lymphocyte subpopulations in neonatal cord blood [25,27,55,56]. However, studies examining these factors reported relative values and not absolute values, which may lead to misinterpretation [28] as we have shown significantly increased numbers of leukocytes in preterm and term infants in the present work.…”

Section: Discussioncontrasting

confidence: 55%

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Avaliação da resposta imune após estimulação de monócitos via Toll-Like Receptor 2 (TLR-2) em recém-nascidos a termo e pré-termo

Faria¹

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Aos meus pais e irmãDedico esta tese aos meus pais Arly e Marlene e à minha irmã Carolina que sempre me apoiaram e me incentivaram a buscar a realização dos meus sonhos, mesmo que fosse necessário ficarmos distantes e que minha ausência perduraria por um longo período. Obrigada por estarem sempre ao meu lado, pelo carinho e amor absoluto.Agradecimento especial À minha orientadora Dra. Patricia Palmeira por mais essa oportunidade oferecida, pela confiança depositada e, principalmente, agradeço pela disponibilidade, pela atenção, pelas conversas, pelos conselhos, por poder contar sempre com o seu apoio. Todos esses anos trabalhando juntas contribuíram decisivamente para o meu crescimento como profissional e como pessoa. AgradecimentosAgradeço, primeiramente, a Deus por me guiar todos os dias, por me dar forças para superar todas as dificuldades e me manter forte para seguir em frente.Este espaço é dedicado a todos aqueles que de forma direta ou indireta contribuíram para a realização deste trabalho. Não sendo possível citar a todos, há, no entanto, alguns a quem não posso deixar de manifestar o meu apreço e agradecimento sincero: À Profa. Dra. Magda Carneiro-Sampaio por ter disponibilizado seu Laboratório permitindo, assim, a realização deste trabalho. Às colegas do Laboratório LIM-36:À Ana Lucia Silveira Lessa, pelo companheirismo e pela ajuda em todos esses anos dedicados ao nosso trabalho. À Camila Rennó, pelo companheirismo, pela sua disponibilidade e contribuição durante o desenvolvimento do trabalho e, principalmente, pela paciência dedicada a mim nos vários momentos de tensão. À Letícia Zicari Boneder, pela alegre convivência e companheirismo e que, mesmo permanecendo por pouco tempo em nosso laboratório, contribuiu para o desenvolvimento deste trabalho.À Laila Lima, pela dedicação e disponibilidade em todos os momentos, agradeço muito por toda a ajuda concedida.

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Section: Discussioncontrasting

confidence: 55%

“…Furthermore, it is difficult to compare previous data because of differences in methodology. Both relative and absolute counts have been used to illustrate the changes in leukocyte subpopulations [25][26][27]. However, it is important to compare absolute counts to assess the actual size of a leukocyte subpopulation [28].…”

Section: Discussionmentioning

confidence: 99%

Avaliação da resposta imune após estimulação de monócitos via Toll-Like Receptor 2 (TLR-2) em recém-nascidos a termo e pré-termo

Faria¹

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“…Therefore we next examined NK cell function and found that the percentage of NK cells in cord blood is lower than that in adults, consistent with the results of Juretic. 37 In addition, perforin expression in neonatal resting NK cells was also significantly lower than that in adult NK cells. Perforin is a pore-forming molecule, and it allows apoptotic molecules such as granulysin to enter cells and cause osmotic lysis of target cells.…”

Section: Correlation Of Antiviral Cytokines Secreted By Nk Cells Withmentioning

confidence: 98%

Impaired NK cell antiviral cytokine response against influenza virus in small-for-gestational-age neonates

Mao

et al. 2013

Cell Mol Immunol

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The neonates, particularly small-for-gestational-age (SGA) ones, are susceptible to various microbial infections. Natural killer (NK) cells are critical components of host innate immunity system and the main source of the inflammatory cytokines, which provide critical protection during the early phase of viral infections before the development of an appropriate adaptive immune response. However, little is known about the antiviral effects of NK cells in neonates especially the SGA population. Herein, a prospective descriptive study was performed to determine the differences of NK cell immunity among adults, appropriate-for gestational-age (AGA) and SGA neonates. Adults have much higher NK cell number in peripheral blood than that in cord blood from neonates. In response to influenza virus stimulation, neonatal NK cells, especially SGA baby cells, expressed significantly lower antiviral cytokines including perforin, interferon (IFN)-c and tumor-necrosis factor (TNF)-a responses than adult NK cells. In addition, the antiviral cytokine responses of NK cells were positively correlated with neonatal birth weight. Our data suggested that the depressed antiviral activity and less frequency of NK cells are likely to be responsible for the high susceptibility to microbial infection in neonates, at least in part. Improving the function of innate immunity may provide a new way to defend virus infection.

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“…All muscle tissue, nerve fibers and energy reserves present in the calf are dependent on the nutritional status of the dam, and thus, it is highly likely that weak calves were born to dams in an undernourished condition [9]. In humans, T cell responses are immature at birth compared with adults [15], and premature neonates demonstrated a significantly lower number of T cells [7,14]. In addition, development of peripheral T or B cells after birth was suppressed in JB calves that were born from dams with insufficient nutrition during the last period of gestation and calves born from primiparous dams [19].…”

mentioning

confidence: 99%

Relationship between Diarrhea and Peripheral Leukocyte Population in Neonatal Japanese Black Calves

Ohtsuka

Mukai²,

Tanami³

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Neonatal Japanese Black (JB) calves show a high incidence of diarrhea. The objective of this study was to analyze the immune cell populations of neonatal JB calves in detail and examine its correlation with the incidence of diarrhea immediately after birth. Understanding the immune cell populations is helpful in clinics in order to determine the condition of the immune system for prevention of diseases. Blood samples were obtained from JB calves on the day of birth. The peripheral leukocyte populations were analyzed separately for calves that had diarrhea within 2 weeks after birth (diarrhea group; n=26) and for calves without diarrhea (control group; n=74). The numbers of the peripheral blood CD3 + TcR1-N12 + and CD8 + T cells were significantly lower in the diarrhea group compared with the control group. These findings suggest that the congenital lower peripheral  and CD8 + T cells results in a high risk of diarrhea in neonatal JB calves.

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Two-Color Flow Cytometric Analysis of Preterm and Term Newborn Lymphocytes

Cited by 31 publications

References 33 publications

Avaliação da resposta imune após estimulação de monócitos via Toll-Like Receptor 2 (TLR-2) em recém-nascidos a termo e pré-termo

Avaliação da resposta imune após estimulação de monócitos via Toll-Like Receptor 2 (TLR-2) em recém-nascidos a termo e pré-termo

Impaired NK cell antiviral cytokine response against influenza virus in small-for-gestational-age neonates

Relationship between Diarrhea and Peripheral Leukocyte Population in Neonatal Japanese Black Calves

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