Two-color Immunofluorescence Flow Cytometric Analysis of Lymphocytes in Long-term Renal Allograft Recipients

Ziegelbaum, Michael; Valenzuela, Rafael; Hayes, Joseph P.; Steinmuller, Donald; Streem, Stevan B.; Novick, Andrew C.

doi:10.1016/s0022-5347(17)40870-6

Cited by 5 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flow-cytometric analysis using monoclonal antibodies is used as an immunologic tool to study peripheral blood lymphocyte subsets before and following renal transplan tation [1,2]. The Finding of an increased number of natu ral killer (NK) cells has generally been seen in limited dis ease processes.…”

Section: Introductionmentioning

confidence: 99%

Successful Renal Transplantation in a Patient with Large Granular Lymphocytic Leukemia with Natural Killer Cell Proliferation

Sibal¹,

Valenzuela²,

Rr³

et al. 1996

Nephron

Self Cite

View full text Add to dashboard Cite

A patient with advancing renal failure attributed to focal segmental glomerulosclerosis was found to have marked NK (CD56+) cell expansion and large granular lymphocytic leukemia. Subsequent living-related renal transplantation was accomplished with two early bouts of acute cellular rejection requiring therapy with methylprednisolone and monoclonal antibody OKT3. Chronic triple drug immunosuppressive therapy has substantially reduced the marked NK (CD56+) proliferation. The renal transplantation remains successful after 15 months despite persistent but reduced numbers of circulating NK cells, the potential role of which in the pathogenesis of the underlying nephropathy or the cellular rejection process remains speculative.

show abstract

Section: Introductionmentioning

confidence: 99%

Successful Renal Transplantation in a Patient with Large Granular Lymphocytic Leukemia with Natural Killer Cell Proliferation

Sibal¹,

Valenzuela²,

Rr³

et al. 1996

Nephron

Self Cite

View full text Add to dashboard Cite

show abstract

“…In most of these investigations, the relative percentage of lymphocyte subsets was studied and not the absolute levels [5,9,10,[12][13][14][15][18][19][20] and the results were often presented in form ofthe ratio between CD4 and CD8 cells [5-7, 10, 13, 14, 21]. Some groups studied the lymphocyte subsets after density gradient isolation [5][6][7]9,10,12,15,[18][19][20][21][22] in spite ofthe risk of selective depletion of a particular T celt subset [23]. In summary, different techniques were used and the variations within different groups of recipients were often large making it difficult to set cut-off limits which discriminate between different patient groups.…”

Section: Introductionmentioning

confidence: 99%

Alterations of the Post Transplant Blood Lymphocyte Phenotype Subsets as a Marker of Rejection in Renal Allograft Recipients

et al. 1995

View full text Add to dashboard Cite

The postoperative alterations of absolute levels of lymphocyte phenotype subsets in peripheral blood were studied in recipients of living donor renal allografts and in kidney donors. The results were expressed as per cent changes of the preoperative values. The lymphocyte subsets, CD3, CD4 and CD8 cells, decreased to approximately 50% following the surgical trauma, with rapid recovery to preoperative levels within 1 week in kidney donors and in recipients without rejection episodes. In contrast, the T-cell levels in recipients with rejection episodes remained low after 1 week, before clinical signs of rejection, and was predictive for the later occurrence of acute rejection episodes. The T-cell levels in the recipients with rejection episodes remained low during the first 6 weeks, maybe due to the rejection treatments given during this period. The B-lymphocytes were not affected in any of the recipient groups. The alterations observed were not explained by CMV infections, which occurred mainly after the observation period of 6 weeks. In conclusion, the operation per se induced alterations in circulating T-lymphocyte subsets and low T-cell levels after 1 week were predictive of rejection episodes.

show abstract

Peripheral blood lymphoid subsets and long-term clinical course of kidney recipients: A longitudinal study

et al. 1998

View full text Add to dashboard Cite

Two-color Immunofluorescence Flow Cytometric Analysis of Lymphocytes in Long-term Renal Allograft Recipients

Cited by 5 publications

References 5 publications

Successful Renal Transplantation in a Patient with Large Granular Lymphocytic Leukemia with Natural Killer Cell Proliferation

Successful Renal Transplantation in a Patient with Large Granular Lymphocytic Leukemia with Natural Killer Cell Proliferation

Alterations of the Post Transplant Blood Lymphocyte Phenotype Subsets as a Marker of Rejection in Renal Allograft Recipients

Peripheral blood lymphoid subsets and long-term clinical course of kidney recipients: A longitudinal study

Contact Info

Product

Resources

About