Two-column gas chromatography of trimethylsilyl derivatives of biochemically significant compounds

Butts, William C.

doi:10.1016/0003-2697(72)90411-3

Cited by 58 publications

(15 citation statements)

References 24 publications

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“…After cooling, the derivatized solution was evaporated to dryness and the residue was re-dissolved in 100 μL chloroform. This solution (1 μL) was analyzed by GC-MS. Silylation derivatization procedures by Butts, (1972) using BSTFA, BSA, or MSTFA formed the basis for derivatisation application that was used to analyze over 200 organic compounds including carboxylic acids, amines, alcohols, phenols, and nucleic acids. The basis of the procedure is as follows: to a 1 -5 mg sample, 100 μL each of pyridine as a solvent and silylating reagent are added.…”

Section: Bis(trimethylsilyl)trifluoroacetamide (Bstfa)mentioning

confidence: 99%

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Derivatization Reactions and Reagents for Gas Chromatography Analysis

Orata¹

2012

Advanced Gas Chromatography - Progress in Agricultural, Biomedical and Industrial Applications

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Section: Bis(trimethylsilyl)trifluoroacetamide (Bstfa)mentioning

confidence: 99%

“…The esters can be eliminated by first forming a methoxime. In the case of Amino acids the reaction may be required to be in a sealed tube or vial (Butts, 1972). The samples are heated cautiously, near the boiling point of the mixture until a clear solution is obtained.…”

Section: Bis(trimethylsilyl)trifluoroacetamide (Bstfa)mentioning

confidence: 99%

Derivatization Reactions and Reagents for Gas Chromatography Analysis

Orata¹

2012

Advanced Gas Chromatography - Progress in Agricultural, Biomedical and Industrial Applications

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“…1971, Holligan and Drew 1971. Similarly, separated amino acids can be determined either colorimetrically (Lee and Takahashi 1966) or by GC (Butts 1972, Kaiser et al 1974, and organic acids either by liquid chromatography (Atkins andCanvin 1971, Prior e/a/. 1973) or GC (Mensen de Silva 1971, Ryan andDupont 1973, Bide 1975).…”

Section: Fractionation Of the Supernatantmentioning

confidence: 99%

Analytical Procedures for the Sequential Extraction of ¹⁴C‐Labeled Constituents from Leaves, Bark and Wood of Cottonwood Plants

Dickson

1979

Physiologia Plantarum

135

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A procedure for the extraction, separation, and measurement of photosynthetically fixed 14C in up to 8 chemical fractions (CHCl), amino acids, organic acids, sugars, protein, starch, hemicellulose, and residue) from small samples (1 to 100 mg) of cottonwood (Populus deltoides Bartr.) leaf material is described. The different chemical fractions are extracted in a sequence of chemical, ion exchange, and enzymatic steps. The 14C‐activity in these major fractions is then determined with liquid scintillation spectrometry. These major fractions (e.g., sugars, amino acids, organic acids) can be further separated into their individual chemical components by standard thin‐layer or gas‐ chromatographic methods for quantitative analysis if specific activities are desired. The major advantage of the procedure is that many chemical fractions can be sequentially separated with good reproducibility from a small amount of plant material without transfer of the material from the original homogenizer or centrifuge tube.

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“…The completeness of the reactions and the stability of the derivatives were not evaluated. 3 Work on the formation of a stable volatile imidazole derivative of a closely related compound, histidine, has apparently not been f r~i t f u l~-~.…”

Section: Introductionmentioning

confidence: 99%

Gas Chromatographic Determination of Microgram and Nanogram Quantities of Histamine as Its N^a-Trifluoroacetyl-N^im-Carbethoxy Derivative

et al. 1976

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A method has been devised for the quantitative estimation of microgram and nanogram quantities of histamine as its Natrifluoroacetyl-N -carbethoxy derivative. Trifluoroacetic anhydride was used for the formation of Na-trifluoroacetyl derivative and ethoxyformic anhydride was used for the N carbethoxy derivative. Quantitative determinations were made in the range of 20 to 100 micrograms and 250 and 1000 nanograms with a mean standard deviation of 2-3%. The standard curves were linear. im im-40 5

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Two-column gas chromatography of trimethylsilyl derivatives of biochemically significant compounds

Cited by 58 publications

References 24 publications

Derivatization Reactions and Reagents for Gas Chromatography Analysis

Derivatization Reactions and Reagents for Gas Chromatography Analysis

Analytical Procedures for the Sequential Extraction of ¹⁴C‐Labeled Constituents from Leaves, Bark and Wood of Cottonwood Plants

Gas Chromatographic Determination of Microgram and Nanogram Quantities of Histamine as Its N^a-Trifluoroacetyl-N^im-Carbethoxy Derivative

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Two-column gas chromatography of trimethylsilyl derivatives of biochemically significant compounds

Cited by 58 publications

References 24 publications

Derivatization Reactions and Reagents for Gas Chromatography Analysis

Derivatization Reactions and Reagents for Gas Chromatography Analysis

Analytical Procedures for the Sequential Extraction of 14C‐Labeled Constituents from Leaves, Bark and Wood of Cottonwood Plants

Gas Chromatographic Determination of Microgram and Nanogram Quantities of Histamine as Its Na-Trifluoroacetyl-Nim-Carbethoxy Derivative

Contact Info

Product

Resources

About

Analytical Procedures for the Sequential Extraction of ¹⁴C‐Labeled Constituents from Leaves, Bark and Wood of Cottonwood Plants

Gas Chromatographic Determination of Microgram and Nanogram Quantities of Histamine as Its N^a-Trifluoroacetyl-N^im-Carbethoxy Derivative