Two complete mitochondrial genomes from Praticolella mexicana Perez, 2011 (Polygyridae) and gene order evolution in Helicoidea (Mollusca, Gastropoda)

Abstract

Helicoidea is a diverse group of land snails with a global distribution. While much is known regarding the relationships of helicoid taxa, comparatively little is known about the evolution of the mitochondrial genome in the superfamily. We sequenced two complete mitochondrial genomes from Praticolella mexicana Perez, 2011 representing the first such data from the helicoid family Polygyridae, and used them in an evolutionary analysis of mitogenomic gene order. We found the mitochondrial genome of Praticolella mexicana to be 14,008 bp in size, possessing the typical 37 metazoan genes. Multiple alternate stop codons are used, as are incomplete stop codons. Mitogenome size and nucleotide content is consistent with other helicoid species. Our analysis of gene order suggested that Helicoidea has undergone four mitochondrial rearrangements in the past. Two rearrangements were limited to tRNA genes only, and two involved protein coding genes.

The complete mitochondrial genome of the terrestrial snail Monacha cartusiana (O.F. Müller, 1774) (Gastropoda, Eupulmonata, Hygromiidae)

“…Colours for the families as in Fig. 2: light blue -Hygromiidae; green -Geomitridae; brown -Polygyridae; red -Helicidae; dark blue -Camaenidae two clades i.e., Bradybaeninae and Camaeninae, treated frequently as two separate families (Lin et al 2016;Minton et al 2016a;Harasewych et al 2017). Our results agree with the division of Helicidae and Camaenidae into subfamilies (Bouchet at al.…”

“…Previous mitogenome phylogenies of stylommatophoran superfamilies (Groenenberg et al 2017;Harasewych et al 2017;Yang et al 2019;Doğan et al 2020) showed a clade of Helicoidea separate from other superfamilies, although mitogenomes of only 11 stylommatophoran superfamilies (Yang et al 2019) out of 26 listed by Bouchet et al (2017) are represented in GenBank. According to Bouchet et al (2017), Helicoidea includes 17 families but hitherto phylogenetic relationships could be analysed only for three or four of them, namely Helicidae, Camaenidae, Geomitridae, and Polygyridae (González et al 2016;Lin et al 2016;Minton et al 2016a;Harasewych et al 2017;Doğan et al 2020).…”

“… 1 Deposited in GenBank as mitogenome of Polygyra cereolus (Megerle von Mühlfeldt, 1818) but according to Minton et al (2016a) it represents Practicolella mexicana Perez, 2011. 2 Mitogenome not mentioned in the paper by Minton et al (2016a) but directly submitted to GenBank ( Minton et al 2016b ). …”

“…Mitogenome sequences are of great importance in molecular phylogenetic studies (Moritz et al 1987), especially to infer evolutionary relationships at species level (Avise et al 1987); this is also the case within Mollusca (Boore 1999). The analysis of mitogenomes may thus provide additional evidence related to stylommatophoran phylogeny (White et al 2011;Parmakelis et al 2013;Minton et al 2016a). The number of available stylommatophoran mitogenomes has increased in the last two decades, from three at the end of the 20 th century (Hatzoglou et al 1995;Terrett et al 1996;Yamazaki et al 1997) to 35 in recent years (Yang et al 2019;Doğan et al 2020).…”

The complete mitogenome of Helix pomatia and the basal phylogeny of Helicinae (Gastropoda, Stylommatophora, Helicidae)

“…From annotated, previously published sequences (accession numbers NC_001816, NC_021747 and NC_017872), we extracted the individual genes following the annotation in the NCBI RefSeq database. However, previous studies, which used the sequence of Ce.nemoralis in mitogenomic phylogenetic analyses, repeatedly resulted in a spuriously long branch for this species (Knudsen et al 2006, González et al 2016, Minton et al 2016, Groenenberg et al 2017). A closer examination of the mitogenome sequence and translation of its protein coding regions revealed a very low quality of the sequence (see also White et al 2011).…”