Two-component dielectric dispersion impedance biosensor for in-line protein monitoring

Oseev, Aleksandr; Schmidt, Marc-Peter; Hirsch, S.; Brose, Andreas; Schmidt, Bertram

doi:10.1016/j.snb.2016.09.118

Cited by 21 publications

(12 citation statements)

References 46 publications

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“…Single chip integrated solutions, such as Lab-on-a-Chip and Micro Total Analysis Systems (μTAS), require the use of compatible approaches to perform the functional elements of analytical systems. These systems allow one to analyze complex liquid probes non-invasively within a single analytical chip, and obtain all necessary information from sample volumes up to the picoliter scale [ 1 , 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

SAW-Based Phononic Crystal Microfluidic Sensor—Microscale Realization of Velocimetry Approaches for Integrated Analytical Platform Applications

Oseev

Lücklum

Zubtsov

et al. 2017

Sensors

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The current work demonstrates a novel surface acoustic wave (SAW) based phononic crystal sensor approach that allows the integration of a velocimetry-based sensor concept into single chip integrated solutions, such as Lab-on-a-Chip devices. The introduced sensor platform merges advantages of ultrasonic velocimetry analytic systems and a microacoustic sensor approach. It is based on the analysis of structural resonances in a periodic composite arrangement of microfluidic channels confined within a liquid analyte. Completed theoretical and experimental investigations show the ability to utilize periodic structure localized modes for the detection of volumetric properties of liquids and prove the efficacy of the proposed sensor concept.

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Section: Introductionmentioning

confidence: 99%

SAW-Based Phononic Crystal Microfluidic Sensor—Microscale Realization of Velocimetry Approaches for Integrated Analytical Platform Applications

Oseev

Lücklum

Zubtsov

et al. 2017

Sensors

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“…Indeed, the dielectric constant is correlated with the polarization of the material [30]. Proteins have a specific interaction within a solvent environment that affects their dielectric relaxation mechanisms [31]. In polar environments there is a reorganization of the solvent's polar molecules along the electric field lines generated by dipoles in the protein [32] and as a consequence, the system relaxes and the protein will emit at longest wavelengths [16].…”

Section: Conformational Analysis Using Fluorescence Spectroscopymentioning

confidence: 99%

Biophysical, photochemical and biochemical characterization of a protease from Aspergillus tamarii URM4634

Silva

Almeida

et al. 2018

International Journal of Biological Macromolecules

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Circular dichroism (CD) and fluorescence spectroscopy (FS) were used to monitor the pH-dependent conformational and structural stability changes induced by temperature and UV light on the protease from Aspergillus tamarii URM4634 at different pH values. The formation of photoproducts, such as N-formylkynurenine, dityrosine and kynurenine, were monitored with FS. The pH-dependent melting temperatures (T) were determined using CD and FS from 20 to 90 °C. Conformational changes were correlated with the pH-dependent biochemical activities. CD revealed that the protease is rich in α-helices. Thermal denaturation was irreversible at all pH range and displayed T values from 42.8 to 67.8 °C (CD) and from 38 to 60.3 °C (FS), which the highest T was observed at pH 6. The light and temperature induced to the formation of photoproducts was more intense at high pH value. Despite the biochemical data shows optimum pH 9, the highest stability was at pH 6, maintaining 100% of activity after 24 h. The acquired data permits to select the best physicochemical parameters to secure the optimal activity and stability when used in biotechnological applications. Furthermore, the conformal changes induced by temperature in the protein are directly correlated with its level of biochemical activity.

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“…Measurements of DNA or protein using fluorescent labels require expensive and precise equipment, and may create an unexpected interference with detection systems. (1)(2)(3)(4)(5)(6) In comparison, electrical sensors enable label-free detection by measuring electrical signals instead of fluorescence brightness. (5,(7)(8)(9)(10)(11) This is a very promising biosensing method owing to the very low detection limit and the relatively rapid completion time.…”

Section: Introductionmentioning

confidence: 99%

Electrode Structure and Stimulation Frequency to Reduce the Estimation Error of Reagent Concentration Determined using Measured Impedance

Kim¹,

Park²,

Park³

et al. 2019

Sensors and Materials

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We present the structure of an electrode and the excitation frequency that reduces the estimation error when the concentration of a reagent is measured using impedance. To compare the performance characteristics of interdigitated electrodes with one to five fingers, five electrodes of the same type were fabricated independently on printed circuit board (PCB) substrates. A fluidic channel was created on each substrate using double-sided tape and a polycarbonate (PC) cover so that the solution was equally distributed over all electrodes. The impedance was measured with stimulation signals of various frequencies for various concentrations. The measured impedance was used to calculate a linear parameter between the concentration and the impedance, and this parameter was used to calculate the error between the estimated value obtained from the impedance and the original concentration. Using this estimation error as a measure, we investigated the electrode and stimulus frequency suitable for each application requiring either lot or individual calibration. Experimental results show that measuring impedance at high frequencies with a single-finger electrode is advantageous for applications requiring lot calibration, while multiple-finger electrodes are suitable for individual calibration applications at low frequencies.

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Two-component dielectric dispersion impedance biosensor for in-line protein monitoring

Cited by 21 publications

References 46 publications

SAW-Based Phononic Crystal Microfluidic Sensor—Microscale Realization of Velocimetry Approaches for Integrated Analytical Platform Applications

SAW-Based Phononic Crystal Microfluidic Sensor—Microscale Realization of Velocimetry Approaches for Integrated Analytical Platform Applications

Biophysical, photochemical and biochemical characterization of a protease from Aspergillus tamarii URM4634

Electrode Structure and Stimulation Frequency to Reduce the Estimation Error of Reagent Concentration Determined using Measured Impedance

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