Two-component signal transduction systems in oral bacteria

Mattos-Graner, Renata O.; Duncan, Marilyn J.

doi:10.1080/20002297.2017.1400858

Cited by 51 publications

(62 citation statements)

References 168 publications

(281 reference statements)

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“…In nature, bacteria are continually subjected to environmental stresses including variations in local pH, osmotic pressure, temperature, redox potential, and nutrient availability, as well as the host immune response . PTMs of proteins are evolutionarily conserved strategies used by organisms to efficiently control their biological activities to sense, adapt, and quickly respond to rapidly changing and hostile environmental challenges .…”

Section: Resultsmentioning

confidence: 99%

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Gong

Zhou

et al. 2019

Molecular Oral Microbiology

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The gram-negative anaerobe Porphyromonas gingivalis is not only a keystone periodontal pathogen but also an emerging systemic pathogen. Although the newly discovered protein post-translational modification (PTM), lysine succinylation (Ksuc), appears to play an important role in modulating metabolic processes in bacteria, this PTM has not been investigated in P gingivalis. In this study, we used a highly sensitive proteomics approach combining affinity enrichment with high-resolution liquid chromatography coupled with tandem mass spectrometry to examine Ksuc in P gingivalis.In total, 345 Ksuc sites in 233 proteins were identified and determined to be involved in a variety of cellular processes. In the region surrounding Ksuc sites, lysine residues were drastically overrepresented and sequence motifs with succinyl-lysine flanked by a lysine at the +3 or +6 positions appear to be unique to this pathogen. Additionally, our results suggest a crosstalk between Ksuc and glycosylation, but the overlap between Ksuc and acetylation in P gingivalis is quite different from that observed in other organisms. Notably, Ksuc was observed in proteins associated with established virulence factors, including gingipains, fimbriae, RagB, and PorR. Moreover, products of the factors necessary for P gingivalis in vitro survival (18.5%) were found to be succinylated at lysine sites and the same was observed in products of fitness factors for P gingivalis survival in both abscess and epithelial cell colonization environments (12%). Collectively, these results suggest that Ksuc may be a new mechanism in modulating the virulence, adaptation, and fitness of P gingivalis. K E Y W O R D S fitness factors, lysine succinylation, necessary factors, Porphyromonas gingivalis, proteomics | 75 WU et al.

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Section: Resultsmentioning

confidence: 99%

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Gong

Zhou

et al. 2019

Molecular Oral Microbiology

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“…In S. mutans, serotype c/e strains isolated from the bloodstream show increased resistance to complement immunity when compared to oral isolates [24,25]. Complement-resistance phenotypes of S. mutans are regulated by the two-component system (TCS) VicRK and CovR [24,25], an orphan response regulator orthologous to the TCS CovRS of Streptococcus pyogenes [26]. Both VicRK and CovR were shown to control the expression of S. mutans genes associated with the synthesis and binding to exopolysaccharides (gtfB/C/D, gbpC, epsC) [27].…”

Section: Introductionmentioning

confidence: 99%

PepO is a target of the two-component systems VicRK and CovR required for systemic virulence of Streptococcus mutans

et al. 2020

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Streptococcus mutans, a cariogenic species, is often associated with cardiovascular infections. Systemic virulence of specific S. mutans serotypes has been associated with the expression of the collagen-and laminin-binding protein Cnm, which is transcriptionally regulated by VicRK and CovR. In this study, we characterized a VicRK-and CovR-regulated gene, pepO, coding for a conserved endopeptidase. Transcriptional and protein analyses revealed that pepO is highly expressed in S. mutans strains resistant to complement immunity (blood isolates) compared to oral isolates. Gel mobility assay, transcriptional, and Western blot analyses revealed that pepO is repressed by VicR and induced by CovR. Deletion of pepO in the Cnm + strain OMZ175 (OMZpepO) or in the Cnm − UA159 (UApepO) led to an increased susceptibility to C3b deposition, and to low binding to complement proteins C1q and C4BP. Additionally, pepO mutants showed diminished ex vivo survival in human blood and impaired capacity to kill G. mellonella larvae. Inactivation of cnm in OMZ175 (OMZcnm) resulted in increased resistance to C3b deposition and unaltered blood survival, although both pepO and cnm mutants displayed attenuated virulence in G. mellonella. Unlike OMZcnm, OMZpepO could invade HCAEC endothelial cells. Supporting these phenotypes, recombinant proteins rPepO and rCnmA showed specific profiles of binding to C1q, C4BP, and to other plasma (plasminogen, fibronectin) and extracellular matrix proteins (type I collagen, laminin). Therefore this study identifies a novel VicRK/CovR-target required for immune evasion and host persistence, pepO, expanding the roles of VicRK and CovR in regulating S. mutans virulence.

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“…Most PCR methods target highly conserved genes within the species to identify S. aureus at the species level [46]. In the present study, PCR targeting a signal transduction gene, vicK of S. aureus was evaluated as a potential marker for [33]. The gene is highly conserved and has been shown to be species-specific [32].…”

Section: Amplification Of Vick Genementioning

confidence: 97%

“…vicK encodes the bacterial two-component signal transduction system (TCS) that senses and responds towards the environmental stimuli especially when invading the host [32]. The gene regulons of TCS are species-specific, which play diverse roles in modulating of cell division, cell-wall biosynthesis and membrane integrity [33]. This study aimed to evaluate vicK as a potential rapid identification marker for S. aureus by determining the frequency of vicK detection in S. aureus clinical isolates.…”

Section: Introductionmentioning

confidence: 99%

<i>vicK</i> Gene as Potential Identification Marker for <i>Staphylococcus aureus</i>

Morad¹,

Misbah²

2018

JBM

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Staphylococcus aureus is an important human pathogen frequently detected in hospital community and has emerged as an important health concern in human medicine. Identification of S. aureus from clinical specimens by phenotypic methods may produce variable characteristics leading to ambiguity.Hence, a rapid and reliable method for identification of S. aureus is required which could expedite appropriate antibiotic therapy. This study aimed to evaluate the specificity of polymerase chain reaction (PCR) targeting a signal transduction gene, vicK, among S. aureus isolates of Hospital Sultanah Nur Zahirah, Kuala Terengganu, Malaysia. A total of 118 bacterial isolates were screened, which consisted of one hundred S. aureus isolates, ten Staphylococcus spp. and eight non-Staphylococci. Results indicated that PCR targeting vicK was able to identify 98% of S. aureus isolates with high sensitivity and specificity, while the remaining isolates of Staphylococcus spp. and non-Staphylococci did not yield any amplification of the gene. vicK thus, is highly specific within interspecies and intraspecies, which is potential to be used as a molecular identification marker for S. aureus.

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Two-component signal transduction systems in oral bacteria

Cited by 51 publications

References 168 publications

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

PepO is a target of the two-component systems VicRK and CovR required for systemic virulence of Streptococcus mutans

<i>vicK</i> Gene as Potential Identification Marker for <i>Staphylococcus aureus</i>

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Two-component signal transduction systems in oral bacteria

Cited by 51 publications

References 168 publications

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

Global analysis of lysine succinylome in the periodontal pathogen Porphyromonas gingivalis

PepO is a target of the two-component systems VicRK and CovR required for systemic virulence of Streptococcus mutans

&lt;i&gt;vicK&lt;/i&gt; Gene as Potential Identification Marker for &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

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<i>vicK</i> Gene as Potential Identification Marker for <i>Staphylococcus aureus</i>