Two conserved Z9-octadecanoic acid desaturases in the red flour beetle, Tribolium castaneum

Horne, Irene; Gibb, Nerida; Damcevski, Katherine; Glover, Karen; Haritos, Victoria S.

doi:10.1016/j.gene.2010.08.003

Cited by 12 publications

(9 citation statements)

References 27 publications

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“…The encoded proteins of the full-length soldier beetle desaturase genes were most closely related to acyl-CoA ∆9 desaturases of the flour beetle, T. castaneum 22 , when subjected to BLASTP analysis against the non-redundant protein NCBI database. The ∆12 desaturase/ acetylenase from C. lugubris (CL10) was found to be unrelated to ∆12 desaturase/acetylenases from the plant C. alpina or fungus C. formosus, as it had amino acid sequence identities of < 12% with these sequences (Supplementary Table S2).…”

Section: Resultsmentioning

confidence: 99%

“…Transformants were selected on SCMM-U plates with 2% d-glucose. Functional characterization of C. lugubris desaturases was conducted in liquid culture, as described 22 , and in the presence of exogenously supplied fatty acids. After galactose addition to a final concentration of 2%, yeast cells were incubated in the presence of fatty acid substrates (final concentration 0.2 mM; Supplementary Table S1).…”

Section: Methodsmentioning

confidence: 99%

“…After galactose addition to a final concentration of 2%, yeast cells were incubated in the presence of fatty acid substrates (final concentration 0.2 mM; Supplementary Table S1). FAME were prepared from yeast and accessory gland lipids using either sulphuric acid-methanol-chloroform or basic methoxide, and analysed by GC/MS as previously described 22 . Briefly, injections were made in the split mode using helium as the carrier gas, and the oven temperature was raised from 60 to 220 °C at 20 °C min − 1 , then to 260 °C at 75 °C min − 1 and held for 6.5 min (method 1) or alternatively from 60 to 180 °C at 20 °C min − 1 , then 190 °C at 2.5 °C min − 1 , then to 260 °C at 25 °C min − 1 and held for 2.2 min (method 2).…”

Section: Methodsmentioning

confidence: 99%

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The convergent evolution of defensive polyacetylenic fatty acid biosynthesis genes in soldier beetles

et al. 2012

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The defensive and bioactive polyacetylenic fatty acid, 8Z-dihydromatricaria acid, is sequestered within a wide range of organisms, including plants, fungi and soldier beetles. The 8Z-dihydromatricaria acid is concentrated in the defence and accessory glands of soldier beetles to repel avian predators and protect eggs. In eukaryotes, acetylenic modifications of fatty acids are catalysed by acetylenases, which are desaturase-like enzymes that act on existing double bonds. Here we obtained acyl Coenzyme A-linked desaturases from soldier beetle RnA and functionally expressed them in yeast. We show that three genes were sufficient for the conversion of a common monounsaturated fatty acid, oleic acid, to the 18 carbon precursor of 8Z-dihydromatricaria acid, that is, 9Z,16Z-octadecadiene-12,14-diynoic acid. These are the first eukaryotic genes reported to produce conjugated polyacetylenic fatty acids. Phylogenetic analysis shows that the genes responsible for 8Z-dihydromatricaria acid synthesis in soldier beetles evolved de novo and independently of the acetylenases of plants and fungi.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The convergent evolution of defensive polyacetylenic fatty acid biosynthesis genes in soldier beetles

et al. 2012

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“…The majority of information is available for one insect group -the moths [18]. FA-modifying enzymes from other insect orders, such as beetles (Coleoptera) [26], flies (Diptera) [27][28][29], crickets (Orthoptera) [30,31] and bees (Hymenoptera) [32][33][34], are substantially less well explored and usually connected to primary FA metabolism rather than pheromone biosynthesis. Two enzyme groups that are encoded by large multigene families in insects -the membrane fatty acyl desaturases (mFADs) [35,36] and the fatty acyl reductases (FARs) [37] -have attracted the most research attention.…”

Section: Insect Enzymes Catalyzing Biosynthesis Of Fa-derived Pheromonesmentioning

confidence: 99%

Biotechnological potential of insect fatty acid-modifying enzymes

Tupec

Buček

Valterová

2017

Zeitschrift Für Naturforschung C

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There are more than one million described insect species. This species richness is reflected in the diversity of insect metabolic processes. In particular, biosynthesis of secondary metabolites, such as defensive compounds and chemical signals, encompasses an extraordinarily wide range of chemicals that are generally unparalleled among natural products from other organisms. Insect genomes, transcriptomes and proteomes thus offer a valuable resource for discovery of novel enzymes with potential for biotechnological applications. Here, we focus on fatty acid (FA) metabolism-related enzymes, notably the fatty acyl desaturases and fatty acyl reductases involved in the biosynthesis of FA-derived pheromones. Research on insect pheromone-biosynthetic enzymes, which exhibit diverse enzymatic properties, has the potential to broaden the understanding of enzyme specificity determinants and contribute to engineering of enzymes with desired properties for biotechnological production of FA derivatives. Additionally, the application of such pheromone-biosynthetic enzymes represents an environmentally friendly and economic alternative to the chemical synthesis of pheromones that are used in insect pest management strategies.

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“…Acyl-CoA desaturase genes from vertebrates, including Siganus canaliculatus (Li et al, 2010), Danio rerio (Hastings et al, 2001) and Homo sapiens (Li et al, 1994) have been cloned. In insects, 3 and 2 acyl-CoA desaturases were identified in Drosophila melanogaster (Wicker-Thomas et al, 1997;Dallerac et al, 2000;Chertemps et al, 2006) and Tribolium castaneum (Horne et al, 2010), respectively. In moths, many fatty acid desaturase genes were cloned from pheromone glands, including Δ11 and/or Δ9 desaturase in Trichoplusia ni (Knipple et al, 1998), Helicoverpa zea (Rosenfield et al, 2001), Bombyx mori (Yoshiga et al, 2000;Moto et al, 2004), Helicoverpa assulta (Jeong et al, 2003), Manduca sexta (Matouskova et al, 2007), and Ostrinia spp ; Δ14 desaturase in Ostrinia spp ; and Δ10 desaturase in Planotortrix octo .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori

Chen¹,

Cheng²,

Liu³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid desaturase genes. Eleven genes contain 3 conserved histidine cluster motifs and 4 transmembrane domains, but their N-terminal residues exhibit obvious diversity. Phylogenetic analysis revealed that there are 6 groups; Bmdesat1 and Bmdesat5-8 were clustered into group 2, which is involved in Δ11 desaturation activity, and Bmdesat3-4 were grouped in group 1, which is involved in Δ9 desaturation activity. Twelve of the 14 genes have expressed sequence tag evidence. Microarray data and reverse transcription polymerase chain reaction analysis demonstrated that Bmdesat3-4 and Bmdesat10 were expressed from the larval to moth stages and in multiple tissues on day 3 of 5th instar larvae. Bmdesat9, Bmdesat11, and Bmdesat14 were expressed during the pupal and late-embryonic stage, suggesting that they may take part in fatty acid metabolism to provide energy. These results provide some insights into

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Two conserved Z9-octadecanoic acid desaturases in the red flour beetle, Tribolium castaneum

Cited by 12 publications

References 27 publications

The convergent evolution of defensive polyacetylenic fatty acid biosynthesis genes in soldier beetles

The convergent evolution of defensive polyacetylenic fatty acid biosynthesis genes in soldier beetles

Biotechnological potential of insect fatty acid-modifying enzymes

Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori

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