Two cotton fiber‐associated glycosyltransferases, <scp>GhGT43A1</scp> and <scp>GhGT43C1</scp>, function in hemicellulose glucuronoxylan biosynthesis during plant development

Li, Long; Huang, Junfeng; Qin, Lixia; Huang, Yuying; Zeng, Wei; Rao, Yue; Li, Juan; Li, Xuebao; Xu, Wen‐Liang

doi:10.1111/ppl.12190

Cited by 30 publications

(31 citation statements)

References 48 publications

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“…The functional module enrichment tool was used to annotate the fiber-specific genes, and several modules related to fiber development were significantly enriched. For example, a NAC transcription factor (45), lipid biosynthesis (46) and sugar and carbohydrate-active enzymes (47) have been reported to play roles in modulating cotton fiber development (Figure 2B). Notably, there was a significant functional module (CFinderADM000212), consisting of eight nodes, that was annotated with ‘cellulose microfibril organization’.…”

Section: Functional Applicationsmentioning

confidence: 99%

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

You

Zhang

et al. 2016

Nucleic Acids Res

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Plant genera with both diploid and polyploid species are a common evolutionary occurrence. Polyploids, especially allopolyploids such as cotton and wheat, are a great model system for heterosis research. Here, we have integrated genome sequences and transcriptome data of Gossypium species to construct co-expression networks and identified functional modules from different cotton species, including 1155 and 1884 modules in G. arboreum and G. hirsutum, respectively. We overlayed the gene expression results onto the co-expression network. We further provided network comparison analysis for orthologous genes across the diploid and allotetraploid Gossypium. We also constructed miRNA-target networks and predicted PPI networks for both cotton species. Furthermore, we integrated in-house ChIP-seq data of histone modification (H3K4me3) together with cis-element analysis and gene sets enrichment analysis tools for studying possible gene regulatory mechanism in Gossypium species. Finally, we have constructed an online ccNET database (http://structuralbiology.cau.edu.cn/gossypium) for comparative gene functional analyses at a multi-dimensional network and epigenomic level across diploid and polyploid Gossypium species. The ccNET database will be beneficial for community to yield novel insights into gene/module functions during cotton development and stress response, and might be useful for studying conservation and diversity in other polyploid plants, such as T. aestivum and Brassica napus.

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Section: Functional Applicationsmentioning

confidence: 99%

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

You

Zhang

et al. 2016

Nucleic Acids Res

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“…Therefore, understanding fiber SCW formation better enables modification of the cell wall for improvements in fiber quality and quantity. Previous studies reported that some cell wall proteins and their related enzymes (e.g., AGPs, PRPs, and glycosyltransferases) are involved in SCW formation of cotton fibers Li et al, 2014;Sun et al, 2015;Xu et al, 2013). Ten members belonging to the cellulose synthase (CesA) family have been identified in cotton, of which CesA1, CesA2, CesA7, and CesA8 are implicated in SCW formation in fibers Li et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Four-week-old seedlings and 8-week-old inflorescence stems of Arabidopsis from the wild-type and transgenic lines were used for total RNA extraction as described previously (Li et al, 2014). Different cotton tissues (including roots, hypocotyls, cotyledons, leaves, stems, petals, anthers, ovules, and fibers) were used for total RNA isolation (Li et al, 2002).…”

Section: Rna Extraction and Rt-pcr Analysismentioning

confidence: 99%

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Cotton GhMYB7 is predominantly expressed in developing fibers and regulates secondary cell wall biosynthesis in transgenic Arabidopsis

Huang

Chen

et al. 2016

Sci. China Life Sci.

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The secondary cell wall in mature cotton fibers contains over 90% cellulose with low quantities of xylan and lignin. However, little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers. In this study, we characterized an R2R3-MYB transcription factor, GhMYB7, in cotton. GhMYB7 is expressed at a high level in developing fibers and encodes a MYB protein that is targeted to the cell nucleus and has transcriptional activation activity. Ectopic expression of GhMYB7 in Arabidopsis resulted in small, curled, dark green leaves and also led to shorter inflorescence stems. A cross-sectional assay of basal stems revealed that cell wall thickness of vessels and interfascicular fibers was higher in transgenic lines overexpressing GhMYB7 than in the wild type. Constitutive expression of GhMYB7 in Arabidopsis activated the expression of a suite of secondary cell wall biosynthesis-related genes (including some secondary cell wall-associated transcription factors), leading to the ectopic deposition of cellulose and lignin. The ectopic deposition of secondary cell walls may have been initiated before the cessation of cell expansion. Moreover, GhMYB7 was capable of binding to the promoter regions of AtSND1 and AtCesA4, suggesting that GhMYB7 may function upstream of NAC transcription factors. Collectively, these findings suggest that GhMYB7 is a potential transcriptional activator, which may participate in regulating secondary cell wall biosynthesis of cotton fibers.cotton (Gossypium hirsutum), fiber development, MYB transcription factor, secondary cell wall (SCW) biosynthesis, ectopic gene expression

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“…xylan synthesis in cotton fiber (Li et al 2014). In this report, we applied a bioinformatics approach aimed at identifying candidate genes as many as possible putatively involved in fiber xylan biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Global identification of genes associated with xylan biosynthesis in cotton fiber

Chen

Guo

Chen

et al. 2020

Preprint

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Background: Mature cotton fiber secondary wall comprises largely of cellulose (>90%) and small amounts of xylan and lignin. Little is known about the cotton fiber xylan biosynthesis by far. Results: To comprehensively survey biosynthetic enzymes involved in xylan biosynthesis in cotton fiber, the combination of the phylogenetic analysis with expression profile analysis and co-expression analyses allowed us to identify five IRX9, five IRX10, one IRX14, six IRX15, two FRA8, one PARVUS, eight GUX, four GXM, two RWA, two AXY9, 13 TBL genes. In addition, we also identified two GT61 members, two GT47 members, and two DUF579 family members whose homologs in Arabidopsis were not functionally characterized. These 55 genes were regarded as the most probable genes to be involved in fiber xylan biosynthesis. Further experimental validation of one IRX10 like and two FRA8 related genes by complementation analysis indicated that these three genes are able to partially recover the irregular xylem phenotype conferred by the xylan deficiency in the respective Arabidopsis mutant. We presume that these genes are functional orthologs of respective genes that are implicated in GX biosynthesis. Conclusion: The list of 55 cotton genes presented here provides a solid basis to uncover the biosynthesis of xylan in cotton fiber, leading to optimization of the cell wall architecture for fiber improvement.

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Two cotton fiber‐associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development

Cited by 30 publications

References 48 publications

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

ccNET: Database of co-expression networks with functional modules for diploid and polyploidGossypium

Cotton GhMYB7 is predominantly expressed in developing fibers and regulates secondary cell wall biosynthesis in transgenic Arabidopsis

Global identification of genes associated with xylan biosynthesis in cotton fiber

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