Two cyp17 genes perform different functions in the sex hormone biosynthesis and gonadal differentiation in Japanese flounder (Paralichthys olivaceus)

Meng, Lihui; Yu, Haiyang; Qu, Junle; Niu, Jingjing; Ni, Feifei; Han, Ping; Wang, Xubo

doi:10.1016/j.gene.2019.02.104

Cited by 7 publications

(9 citation statements)

References 37 publications

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“…FSH treatment was found to increase the transcript level of cyp17a1, along with star and hsd3b genes (Rolland et al 2013) whereas androgens downregulated the mRNA expression level of such transcripts. Androgens and oestrogens downregulated the cyp17 isoforms in Japanese flounder, (Meng et al 2019) whereas the gonadotropin hormone treatment upregulated the mRNA levels of cyp17 in female eels (Matsubara et al 2019). The decrease in the expression level of cyp17 with the increased use of androgens may be due to negative feedback effect on HPG axis, which downregulates the neurohormones important for the initiation of steroidogenesis (Bidlingmaier & Knorr 1981).…”

Section: Starmentioning

confidence: 99%

“…2007; Meng et al . 2019). This transcript is more expressed in the fish gonads and has been isolated from rainbow trout, zebrafish and flathead minnow (Sakai et al .…”

Section: Genes Involved In Steroidogenesis Pathway and Their Modulation In Fishmentioning

confidence: 99%

“…Other than gonads, the transcript is expressed in the brain, liver, head kidney, gills and intestine thereby helping in the steroid production in extra‐gonadal tissues (Wang & Ge 2004; Meng et al . 2019).…”

Section: Genes Involved In Steroidogenesis Pathway and Their Modulation In Fishmentioning

confidence: 99%

“…cyp17 cyp17 or P450c17 is an important enzyme used for conversion of pregnenolone into 17a-hydroxypregnenolone and later to dehydroepiandrosterone, and also progesterone into 17a-hydroxyprogesterone and androstenedione (Miller et al 1997). In fish, two isoforms of star have been characterized, cyp17I and cyp17II (Sakai et al 1992;Filby et al 2007;Zhou et al 2007;Meng et al 2019). This transcript is more expressed in the fish gonads and has been isolated from rainbow trout, zebrafish and flathead minnow (Sakai et al 1992;Halm et al 2003;Wang & Ge 2004;Filby et al 2007).…”

Section: Starmentioning

confidence: 99%

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Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Bhat

Dar

Ahmad

et al. 2021

Reviews in Aquaculture

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In fish, as in higher animals, the normal ontogenesis of the gonads is the basis for successful reproduction. Gonadal differentiation and development depend on the genetic cascade that programmes the events. Eventually, gonads become the sites for the maturation of gametes, which are originally derived from the primordial germ cells (PGCs) and form the cellular basis of reproduction. In fish hormones of the hypothalamic-pituitary-gonadal (HPG) axis regulate reproduction and fertility by promoting or inhibiting the production of gonadal sex steroids and their function. Gonadal sex steroids not only guide proper gonadal development and function but also play a key role in the activation and regulation of the HPG axis through two feedback control systems. In this review, we discuss the general outline and expression pattern of the major genes involved in testicular development and gonadal steroidogenesis in fish. The review attempts, for the first time, to collect and discuss recent information regarding the regulation of sex differentiation and steroidogenic genes during the stages of testicular development and in response to different external factors. Furthermore, clear insights into the important genes and their regulation involved in the HPG axis and spermatogenesis are presented briefly. This review can serve as a guide for fish breeders on the substances that have a positive impact on reproduction in males.

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Section: Starmentioning

confidence: 99%

“…2007; Meng et al . 2019). This transcript is more expressed in the fish gonads and has been isolated from rainbow trout, zebrafish and flathead minnow (Sakai et al .…”

Section: Genes Involved In Steroidogenesis Pathway and Their Modulation In Fishmentioning

confidence: 99%

Section: Genes Involved In Steroidogenesis Pathway and Their Modulation In Fishmentioning

confidence: 99%

Section: Starmentioning

confidence: 99%

See 2 more Smart Citations

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Bhat

Dar

Ahmad

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

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“…MT is commonly found in sewage from paper mill and domestic and livestock manure, especially in aquatic reproduction; approximately 1.33 ng/L of MT was detected in the sewage discharge of chemical plants, while 4.1-7.0 ng/L MT was detected in Beijing wastewater samples. MT can inhibit the activity of steroidogenic enzymes, especially aromatase (Kortner and Arukwe, 2007), and cause alterations in sex steroid hormone levels in the body (Lyu et al, 2019;Meng et al, 2019). MT exposure can, thus, lead to sex reversal in many kinds of fish, such as zebrafish (Lee et al, 2017) and orange-spotted grouper (Wang et al, 2018;Huang et al, 2019;Lyu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of mRNA- and miRNA-Seq in the Ovary of Rare Minnow Gobiocypris rarus in Response to 17α-Methyltestosterone

et al. 2021

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17α-Methyltestosterone (MT) is a synthetic androgen. The objective of this study was to explore the effects of exogenous MT on the growth and gonadal development of female rare minnow Gobiocypris rarus. Female G. rarus groups were exposed to 25–100 ng/L of MT for 7 days. After exposure for 7 days, the total weight and body length were significantly decreased in the 50-ng/L MT groups. The major oocytes in the ovaries of the control group were vitellogenic oocytes (Voc) and cortical alveolus stage oocytes (Coc). In the MT exposure groups, some fish had mature ovaries with a relatively lower proportion of mature oocytes, and the diameter of the perinucleolar oocytes (Poc) was decreased compared with those of the control group. Ovarian VTG, FSH, LH, 11-KT, E2, and T were significantly increased after exposure to 50 ng/L of MT for 7 days. Unigenes (73,449), 24 known mature microRNAs (miRNAs), and 897 novel miRNAs in the gonads of G. rarus were found using high-throughput sequencing. Six mature miRNAs (miR-19, miR-183, miR-203, miR-204, miR-205, and miR-96) as well as six differentially expressed genes (fabp3, mfap4, abca1, foxo3, tgfb1, and zfp36l1) that may be associated with ovarian development and innate immune response were assayed using qPCR. Furthermore, the miR-183 cluster and miR-203 were differentially expressed in MT-exposed ovaries of the different G. rarus groups. This study provides some information about the role of miRNA–mRNA pairs in the regulation of ovarian development and innate immune system, which will facilitate future studies of the miRNA–RNA-associated regulation of teleost reproduction.

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Molecular identification of a steroidogenesis-related gene, star3, and its function in gonadal development of Hyriopsis cumingii

Gu,

Shangguan,

Mao

et al. 2024

Aquaculture Reports

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Two cyp17 genes perform different functions in the sex hormone biosynthesis and gonadal differentiation in Japanese flounder (Paralichthys olivaceus)

Cited by 7 publications

References 37 publications

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Integrated Analysis of mRNA- and miRNA-Seq in the Ovary of Rare Minnow Gobiocypris rarus in Response to 17α-Methyltestosterone

Molecular identification of a steroidogenesis-related gene, star3, and its function in gonadal development of Hyriopsis cumingii

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