Two decades of genetic consistency in a reproductive population in the face of exploitation: patterns of adult and larval walleye (Sander vitreus) from Lake Erie’s Maumee River

Haponski, Amanda E.; Stepien, Carol A.

doi:10.1007/s10592-016-0866-x

Cited by 6 publications

(9 citation statements)

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“…Genomic DNA was extracted, purified, and amplified using the PCR, and allelic variation was assessed at 14 nuclear DNA microsatellite loci (see Table ) following previously published procedures (Stepien et al. , ; Haponski and Stepien , ). Amplification products were diluted 1:50, of which 1 μL was added to 13 μL of formamide and Applied Biosystems (ABI, Fullerton, California) Gene Scan 500liz size standard in 96‐well plates, denatured for 2 min at 95°C, and analyzed on an ABI 3130xl Genetic Analyzer with GENEMAPPER version 3.7.…”

Section: Methodsmentioning

confidence: 99%

“…From the mid‐1990s to the present, Walleye spawning groups in the western and eastern basins of Lake Erie each have maintained their respective genetic consistency, composition, and diversity (Stepien et al. , ; Haponski and Stepien ). Spawning groups from Lake Erie's central basin have not previously been investigated for fine‐scale differences or genetic continuity over time, largely due to the smaller size of the stocks, stochasticity in the runs (WTG ), and corresponding lack of samples.…”

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confidence: 99%

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Genetic Divergence of Nearby Walleye Spawning Groups in Central Lake Erie: Implications for Management

Stepien

Snyder

Knight

2018

N American J Fish Manag

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Spawning-site philopatry may lead to genetic differentiation among reproductive populations, despite their locations being in close proximity within single bodies of water. Identifying and maintaining locally differentiated spawning groups of Walleye Sander vitreus constitute an agency management priority of the multi-agency Walleye Task Group advisory for the Great Lakes Fishery Commission. Although genetically separable spawning groups of Walleyes have been identified from several areas in the Great Lakes, those in central Lake Erie were previously unknown. The Ohio Division of Wildlife (ODW) collected Walleyes for the present analysis from two spawning groups in Lake Erie's central basin, located just 2 km apart-one in the Grand River, Ohio, and the other at the nearby central basin reef. The hypothesis of whether the two spawning groups were genetically different was tested by analyzing variation at 14 nuclear DNA microsatellite loci from Walleyes (n = 147) reproducing in 2012 at the two sites and among three separate years (1996, 2003, and 2012) in the Grand River (to evaluate temporal trends). Results revealed relatively high genetic diversity in both spawning groups, with the reproductive population in the Grand River having significantly greater allelic richness and representation of private alleles. The Grand River Walleye runs showed a slight temporal decline in allelic richness from 1996 to 2003, which coincided with ODW reports of declining numbers of Walleyes reproducing there. The two spawning groups differed in genetic composition suggesting that they are closely related, yet are separable reproductive subpopulations, with both contributing to the overall diversity of Lake Erie Walleye. Their maintenance and integrity thus may need management attention and further monitoring.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Genetic Divergence of Nearby Walleye Spawning Groups in Central Lake Erie: Implications for Management

Stepien

Snyder

Knight

2018

N American J Fish Manag

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“…Lake Erie is known as the "walleye capital of the world", supporting substantial commercial and recreational fisheries worth ~$2 billion US annually [42]. Stepien et al [43][44][45] and Haponski and Stepien [46] conducted studies to evaluate genetic differences among walleye spawning groups across Lake Erie and other sites, as well as the genetic consistency and diversity of walleye returning to given spawning locations, which return annually to the same areas in rivers and shallow water reefs. Findings showed that these spawning groups have maintained genetic stability and high diversity over the past two decades, despite intensive anthropogenic pressures.…”

Section: ) Habitat Loss and Wetlandsmentioning

confidence: 99%

“…By 1984, Lake Erie walleye had rebounded to ~90 million, which is 9 times the 1978 population; these numbers since have decreased to ~25 million in 2014[50]. Genetic analyses[44][46] have revealed temporal consistency in genetic diversity and allelic composition in Lake Erie walleye spawning groups from 1995 to the present day, despite intensive anthropogenic pressures.…”

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confidence: 99%

Enhancing Environmental Sustainability Through A University Field Station

Stepien¹,

Calzonetti²,

Bossenbroek³

et al. 2017

Sustainability Practice and Education on University Campuses and Beyond

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University field stations are located off site from the main campuses and frequently in a natural setting, providing opportunity for students, faculty, and the public to engage with-and appreciate-local ecosystems. Their missions usually encompass the three cornerstones of environmental research, education, and outreach/community engagement, which go hand-in-hand with understanding and furthering sustainability. University field stations enhance environmental sustainability by helping to preserve a natural setting for coming generations, fostering research and monitoring of local ecosystems and their component biodiversity, and training the next generation and citizen scientists for field and laboratory work. Here we provide an example of how we are addressing sustainability through growth of the Lake Erie Center, a mid-sized university center with modest funding and staff that is located at the heart of land-water issues of runoff, sedimentation, algal blooms, legacy contaminants, and habitat loss facing the world's largest freshwater ecosystem. We have networked our mission by building an Environmental Science Learning Community, which brings together faculty, students, educators, agencies, stakeholders, and the public to work towards the common goal of improving land-lake ecosystem services. This background has allowed us to rapidly respond to the August 2014 "Toledo Water Crisis" in which the Lake Erie water supply to 500,000 local citizens was contaminated by the algal toxin microcystin, resulting in a "do not drink" health advisory. The Lake Erie Center's strategic location, both geographically and scientifically, has enhanced our effective education, research, and community engagement programs.

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“…Specimens too degraded for classical PCR and Sanger sequencing can now be integrated through the use of next generation sequencing (NGS) technologies (Bi et al, 2013;Guschanski et al, 2013;Besnard et al, 2016). These new methods have been applied to studies of the evolutionary history and phylogeography of a variety of vertebrate (e.g., McCormack et al, 2012;Besnard et al, 2016;Melville et al, 2017) and invertebrate taxa (e.g., Haponski and Stepien, 2016;Yuan et al, 2016;Allen et al, 2017) and to explore the microbiomes of a wide range of animals (e.g., Phillips et al, 2012;Bourne et al, 2013;Carrillo-Araujo et al, 2015;Jiménez and Sommer, 2017). Our study extends this approach to characterise communities of helminth parasites in preserved vertebrate specimens.…”

Section: Introductionmentioning

confidence: 99%

Museum metabarcoding: A novel method revealing gut helminth communities of small mammals across space and time

Greiman

Cook

Tkach

et al. 2018

International Journal for Parasitology

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Natural history collections spanning multiple decades provide fundamental historical baselines to measure and understand changing biodiversity. New technologies such as next generation DNA sequencing have considerably increased the potential of museum specimens to address significant questions regarding the impact of environmental changes on host and parasite/pathogen dynamics. We developed a new technique to identify intestinal helminth parasites and applied it to shrews (Eulipotyphla: Soricidae) because they are ubiquitous, occupy diverse habitats, and host a diverse and abundant parasite fauna. Notably, we included museum specimens preserved in various ways to explore the efficacy of using metabarcoding analyses that may enable identification of helminth symbiont communities from historical archives. We successfully sequenced the parasite communities (using 12S mtDNA, 16S mtDNA, 28S rDNA) of 23 whole gastrointestinal tracts. All gastrointestinal tracts were obtained from the Museum of Southwestern Biology, USA, and from recent field collections, varying both in time since fixation (ranging from 4 months to 16 years) and preservation method (70% or 95% ethanol stored at room temperature, or flash frozen in liquid nitrogen and stored at À80°C). Our proof of concept demonstrates the feasibility of applying next generation DNA sequencing techniques to authoritatively identify the parasite/pathogen communities within whole gastrointestinal tracts from museum specimens of varying age and fixation, and the value of future preservation of host-associated whole gastrointestinal tracts in public research archives. This powerful approach facilitates future comparative examinations of the distributions and interactions among multiple associated groups of organisms through time and space.

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Two decades of genetic consistency in a reproductive population in the face of exploitation: patterns of adult and larval walleye (Sander vitreus) from Lake Erie’s Maumee River

Cited by 6 publications

References 73 publications

Genetic Divergence of Nearby Walleye Spawning Groups in Central Lake Erie: Implications for Management

Genetic Divergence of Nearby Walleye Spawning Groups in Central Lake Erie: Implications for Management

Enhancing Environmental Sustainability Through A University Field Station

Museum metabarcoding: A novel method revealing gut helminth communities of small mammals across space and time

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