Two different primary oxidation mechanisms during biotransformation of thymol by gram-positive bacteria of the genera Nocardia and Mycobacterium

Hahn, Veronika; Sünwoldt, Katharina; Mikolasch, Annett; Schauer, Frieder

doi:10.1007/s00253-012-4293-8

Cited by 12 publications

(6 citation statements)

References 33 publications

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“…The Isolates AN18, AN37 and AN47 were identified as N. cyriacigeorgica. This species has been reported for degradation capacity of PAHs and thymol 34 , 35 . The isolates AN31and AN32 were identified as N. asteroides .…”

Section: Resultsmentioning

confidence: 59%

“…N. farcinica was the first historically identified Nocardia species and based on the result of current study and previous studies has the ability to degrade the paraffin wax, crude oil and phenol 32 , 33 . N. cyriacigeorgica ranked second that included 16% of the isolates, this organism that was first isolated in 2001 from clinical specimens 47 has been reported to be able to degrade the PAHs and thymol 34 , 35 .…”

Section: Discussionmentioning

confidence: 99%

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Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems

Azadi

Shojaei

2020

Sci Rep

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Anthropogenic pollutants are known to have adverse effect on ecosystem, biodiversity and human health. Bioremediation is an option that has been widely used to remediate organic contaminants and reduce the risk of these hazardous materials. Microorganisms are readily available to screen and can be rapidly characterized to be applied in many extreme environmental conditions. Actinomycetes have a great potential for the production of bioactive secondary metabolites which have biodegradation activity. This study aimed to screen and characterize Nocardia species with biodegradation potential from diverse Iranian ecosystems. The isolates were screened from 90 collected environmental samples, identified and characterized using conventional and molecular microbiological methods including the PCR amplification and sequencing analysis of 16S rRNA and rpoB genetic markers. Growth rate in presence of pollutants, chromatography, Gibbs and turbidometric methods were used to determine bioremediation ability. A total of 19 Nocardia isolates were recovered from the cultured samples (21.1%) that belonged to 10 various species. The most prevalent Nocardia species was N. farcinica; 4 isolates (21%), followed by N. cyriacigeorgica and N. cashijiensis like; 3 isolates each (15.7%) and N. asteroides and N. kroppenstedtii; 2 isolates each (10.5%). Our results showed that various Nocardia species have great potential for bioremediation purposes, although they have not received much attention of the scholars for such significant usage.

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Section: Resultsmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems

Azadi

Shojaei

2020

Sci Rep

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“…It is worth noting that these compounds could be degraded in the environment by photolysis, mineralization, as well as by aerobic and anaerobic microorganisms. (Chamberlain and Dagley, 1968;Tadasa, 1977;Gonz alez et al, 1993;Rabenhorst, 1996;Peir o et al, 2001;Pracht et al, 2001;Overhage et al, 2002;Amat et al, 2005;Ashengroph et al, 2011;Samet et al, 2011;Hahn et al, 2013;Mishra et al, 2013).…”

Section: Discussionmentioning

confidence: 96%

Evaluation of low copper content antifouling paints containing natural phenolic compounds as bioactive additives

Pérez

Garcia

Blustein

2015

Marine Environmental Research

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“…Es importante destacar que el timol es un compuesto que puede sufrir distintos procesos de biotransformación en el medio ambiente por medio de una amplia variedad de organismos, entre ellos, bacterias, hongos y hasta plantas superiores [18][19][20][21]. Chamberlain y Dagley [18] demostraron que Pseudomonas putida es capaz de degradar al timol en ácidos carboxílicos y cetoácidos sencillos a través de una ruta metabólica que involucra a varios metabolitos intermediarios, entre ellos anillos bencénicos di y trihidroxilados y con estructura quinoide.…”

Section: Figura 1: Estructura Del Timolunclassified

“…Chamberlain y Dagley [18] demostraron que Pseudomonas putida es capaz de degradar al timol en ácidos carboxílicos y cetoácidos sencillos a través de una ruta metabólica que involucra a varios metabolitos intermediarios, entre ellos anillos bencénicos di y trihidroxilados y con estructura quinoide. Hahn y colaboradores [19] han reportado que bacterias Grampositivas de los géneros Nocardia y Mycobacterium degradan al timol mediante dos diferentes mecanismos oxidativos y que los productos obtenidos son menos tóxicos que el propio timol. Por otra parte, se ha encontrado que varios géneros de hongos, en particular Colletotrichum acutatum y Botryodiplodia theobromae también degradan al timol dando una compleja mezcla de productos de degradación [20].…”

Section: Figura 1: Estructura Del Timolunclassified

Hacia una reducción del contenido decobre en pinturas antiincrustantes

et al. 2015

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RESUMENEl biofouling conduce al deterioro de las estructuras emplazadas en el mar acarreando graves pérdidas económicas. La aplicación de pinturas a base de óxido cuproso es el método más difundido para el control de las incrustaciones aún siendo nocivas para los ecosistemas marinos. Ante la necesidad de desarrollar formulaciones más inocuas se propone el uso de productos de origen natural que puedan reemplazar parte del cobre disminuyendo su aporte al medio ambiente.El timol se aísla de los aceites esenciales de numerosas plantas (tomillo, orégano, albahaca) y ha sido ampliamente estudiado por sus propiedades antimicrobianas. La hipótesis que se plantea es que inhibiría el proceso inicial del asentamiento de organismos incrustantes.El objetivo de este trabajo es reducir el contenido de cobre en las pinturas antifouling empleando al timol como aditivo.Se prepararon dos pinturas antifouling, una conteniendo 16% v/v de cobre y otra con 1,6% de cobre + 2% de timol. Las pinturas se aplicaron sobre paneles de acrílico y se sumergieron en el puerto de Mar del Plata. Luego de 6 meses los paneles se retiraron y observaron en el laboratorio bajo lupa binocular y microscopio óptico. La estimación de la fijación para cada especie sobre los paneles se evaluó con una grilla de 25 puntos al azar. Se trabajó por triplicado, con controles y se aplicaron test estadísticos.Las pinturas con cobre+timol presentaron una muy buena performance dado que disminuyeron el asentamiento del biofouling. No se registraron diferencias significativas en los porcentajes de cobertura entre las formulaciones con cobre y la pintura con cobre+timol (p>0,05). El timol empleado como aditivo en la formulación antiincrustante permitió reducir 90% el contenido de cobre manteniendo la efectividad en servicio. Palabras clave: incrustaciones biológicas, timol, óxido cuproso, pinturas antiincrustantes ABSTRACTBiofouling leads to deterioration of any submerged material. The most widespread method for control is the application of cuprous oxide antifouling paints which are toxic. For this reason non-toxic natural products could be combined with small amounts of copper to diminish its contribution to the environment.Thymol is isolated from essential oils of several plants (thyme, oregano, basil) and it is well known for its antimicrobial properties. The hypothesis proposed in this study is this compound would inhibit initial steps in fouling sequence and consequently would also inhibit settlement of later species.The aim of this work was to reduce the amount of copper in antifouling paints employing thymol as an additive. Two antifouling paints were prepared, one containing 16% v/v copper and another with 1.6% copper + 2% thymol. Paints were applied on acrylic panels and were submerged in Mar del Plata harbor.After 6 months of immersion in Mar del Plata harbor fouling cover percentage on panels was estimated under stereomicroscope and optic microscope. Estimation of fouling cover by species was carried out using a 25 random point grid. Also, control paints and ...

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Two different primary oxidation mechanisms during biotransformation of thymol by gram-positive bacteria of the genera Nocardia and Mycobacterium

Cited by 12 publications

References 33 publications

Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems

Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems

Evaluation of low copper content antifouling paints containing natural phenolic compounds as bioactive additives

Hacia una reducción del contenido decobre en pinturas antiincrustantes

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