Two different types of surface structures of methane utilizing bacteria

Haubold, R.

doi:10.1002/jobm.19780180707

Cited by 8 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4C, arrow). These structures agree with Haubold's (1978) description of globules in BG8 by FE and negative staining. We are able to identify these globules in cross fracture (Fig.…”

Section: Albus Bg8supporting

confidence: 90%

Effect of fixation‐resin combinations and ruthenium red on elucidating outer envelope structure and surface morphology of two methanotrophic bacteria

Fassel

Schaller²,

Lidstrom

et al. 1990

J. Elec. Microsc. Tech.

View full text Add to dashboard Cite

We examined the ultrastructure of the cell envelope in Type I, Methylomonas albus (BG8), and Type II, Methylosinus trichosporium (OB3b), methane-oxidizing bacteria by using different fixatives, ruthenium red (RR) combinations and resins. We compared LR White and Spurr embedments with the following fixations: glutaraldehyde/OsO4, two glutaraldehyde-paraformaldehyde, and two different en bloc ruthenium red procedures, one utilizing OsO4 and the other with glutaraldehyde/OsO4 in sequential fixation. These fixations were also studied by scanning electron microscopy (SEM). Unfixed cells prepared by freeze etch were used for comparison. Transmission electron microscopy of BG8 embedded in LR White resin (with or without red0 preserved a layer of cup-like structures that were not seen in Spurr resin-embedded cells unless ruthenium red was used. For OB3b, the second RR method preserved beads and filaments where only "spike-like" structures were seen in all other fixations in both resins. By SEM, all fixations preserved a capsular slime layer of BG8 that was removed from some cells by both RR methods. In all SEM fixations, a bead layer was preserved in OB3b that was enhanced by RR. Filaments seen by freeze-etch and thin-section techniques were not seen in SEM. Presence or absence of particular envelope structures in these methanotrophs is dependent on the combination of fixatives and/or resins employed and is species-specific. The chemical preparation methods used resulted in enhanced understanding of the structure and composition of the cell envelope.

show abstract

“…4C, arrow). These structures agree with Haubold's (1978) description of globules in BG8 by FE and negative staining. We are able to identify these globules in cross fracture (Fig.…”

Section: Albus Bg8supporting

confidence: 90%

Effect of fixation‐resin combinations and ruthenium red on elucidating outer envelope structure and surface morphology of two methanotrophic bacteria

Fassel

Schaller²,

Lidstrom

et al. 1990

J. Elec. Microsc. Tech.

View full text Add to dashboard Cite

show abstract

“…The epithet echinoides is a Neo-Latin adjective derived from the Greek word echinos, meaning hedgehog, named for the hedgehog-like appearance of this bacterium. However, as reported by Gal'chenko et al (1977) and Haubold (1977) and verified in this study (Fig. 3c, d), the spines on these methanotrophs appear to be tubular and less dense in comparison with the spikes observed on strain CSC1 T , which would be more aptly named for a hedgehog.…”

supporting

confidence: 87%

“…3c) that appear striated at high magnification (Fig. 3d), as reported by Haubold (1977) for this strain but not in the original description of Gal'chenko et al (1977). Haubold (1977) concluded that the tubes of Methylocystis echinoides IMET 10491 T were smaller in diameter than those described by Gal'chenko et al (1977).…”

supporting

confidence: 55%

Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer

Lindner

Pacheco

Aldrich

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Strain CSC1T , a Gram-negative, aerobic, methane-oxidizing bacterium, was isolated from an uncontaminated aquifer nearly 20 years ago. Based on 16S rRNA gene sequence similarity, this strain was identified as a member of the Alphaproteobacteria, most closely related to an uncultured member of the Methylocystaceae as well as two cultured organisms, Species of the genus Methylocystis are strictly aerobic, Gram-negative bacteria that are able to grow on onecarbon compounds (e.g. methane or methanol) (Bowman et al., 1993). The genus Methylocystis belongs to the class Alphaproteobacteria and currently consists of three species with standing in nomenclature, Methylocystis parvus, Methylocystis echinoides and Methylocystis rosea (Whittenbury et al., 1970;Gal'chenko et al., 1977;Bowman et al., 1993; Wartainen et al., 2006). Numerous Methylocystis strains have been identified in a variety of environments, including lake, ocean, marsh and creek sediments and water, coal mine drainage water and the roots of plants (Whittenbury et al., 1970;Gal'chenko et al., 1977;Bowman et al., 1993;Hanson & Hanson, 1996;Calhoun & King, 1998;Heyer et al., 2002 (Stanley et al., 1983;Prior & Dalton, 1985;Choi et al., 2003;Wartiainen et al., 2006). Methylocystis parvus OBBP T and Methylocystis rosea SV97 T do not possess genes encoding sMMO (Tsien & Hanson, 1992;Lloyd et al., 1999;Wartiainen et al., 2006). The genera Methylocella and Methylocapsa are classified as type II methanotrophs; however, they possess morphological and Abbreviations: PHB, poly-b-hydroxybutyrate; pMMO, particulate methane mononoxygenase; sMMO, soluble methane monooxygenase; TEM, transmission electron microscopy. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and partial mxaF, mmoX and pmoA gene sequences of strain CSC1T are respectively DQ364433, DQ664499, DQ664498 and DQ364434.Neighbour-joining trees based on partial mmoX and mxaF gene sequences and results of electron microscope cytochemistry of the S-layer of strain CSC1T are available as supplementary material with the online version of this paper.

show abstract

“…Spiral, tubular appendages perpendicular to and distributed over the outer surface have been observed in Methylocystis echinoides (sp nov.) (160) and Methylocystis sp. strain IC493S/5 (55).…”

Section: Morphology and Physiologymentioning

confidence: 99%

Methane-oxidizing microorganisms.

Higgins¹,

Best²,

Hammond³

et al. 1981

Microbiological Reviews

139

View full text Add to dashboard Cite

Fine structure of type I methane-utilizing bacteria .559 Fine structure of type II methane-utilizing bacteria .559 Effect of growth conditions on intracytoplasmic membranes .560 Phospholipid and fatty acid compositions of methanotrophs560 Exospore and cyst formation by methanotrophs .562 Ultrastructure of methane-utilizing yeasts .563 CARBON METABOLISM .563 The Serine Pathway .563 The Ribulose Monophosphate (Quayle) Cycle and Other Aspects of Carbon Assimilation by Type I Methanotrophs .567 The Dissimilatory Ribulose Monophosphate Pathway .570

show abstract

Two different types of surface structures of methane utilizing bacteria

Abstract: Methylocystis spec., strain IC 493s/5, and Methylomonas albus WHITTENBURY, strain BG 8 (WHITTENBURY et al. 1970), are shown to form surface structures unusual with methane utilizing bacteria.

Cited by 8 publications

References 13 publications

Effect of fixation‐resin combinations and ruthenium red on elucidating outer envelope structure and surface morphology of two methanotrophic bacteria

Effect of fixation‐resin combinations and ruthenium red on elucidating outer envelope structure and surface morphology of two methanotrophic bacteria

Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer

Methane-oxidizing microorganisms.

Contact Info

Product

Resources

About