Two differentially structured collagen scaffolds for potential urinary bladder augmentation: proof of concept study in a Göttingen minipig model

Leonhäuser, Dorothea; Stollenwerk, K.; Seifarth, Volker; Zraik, Isabella; Vogt, Michael; Srinivasan, Pramod Kadaba; Tolba, René; Grosse, Joachim

doi:10.1186/s12967-016-1112-5

Cited by 19 publications

(17 citation statements)

References 42 publications

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“…Most of these methods use cell-seeded matrices to build tissue-engineered tubular grafts 11 , 12 . New, biologically derived scaffolds seeded with autologous cells for bladder wall substitution are also investigated 13 – 15 . Several cell types, e.g.…”

Section: Introductionmentioning

confidence: 99%

The interplay between adipose-derived stem cells and bladder cancer cells

Maj

Kokocha

Bajek

et al. 2018

Sci Rep

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Tissue engineering approaches offer alternative strategies for urinary diversion after radical cystectomy. Possible triggering of cancer recurrence remains, however, a significant concern in the application of stem-cell based therapies for oncological patients. Soluble mediators secreted by stem cells induce tissue remodelling effects, but may also promote cancer cells growth and metastasis. We observed a substantial increase in the concentration of IL-6 and IL-8 in the secretome of adipose-derived stem cells (ASCs) co-cultured with bladder cancer cells. Concentrations of GM-CSF, MCP-1 and RANTES were also elevated. Bioactive molecules produced by ASCs increased the viability of 5637 and HT-1376 cells by respectively 15.4% and 10.4% (p < 0.0001). A trend in reduction of adhesion to ECM components was also noted, even though no differences in β-catenin expression were detected. When HT-1376 cells were co-cultured with ASCs their migration and invasion increased by 24.5% (p < 0.0002) and 18.2% (p < 0.002). Expression of p-ERK1/2 increased in 5637 cells (2.2-fold; p < 0.001) and p-AKT in HB-CLS-1 cells (2.0-fold; p < 0.001). Our results confirm that ASCs crosstalk with bladder cancer cells in vitro what influences their proliferation and invasive properties. Since ASCs tropism to tumour microenvironment is well documented their application towards post-oncologic reconstruction should be approached with caution.

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Section: Introductionmentioning

confidence: 99%

The interplay between adipose-derived stem cells and bladder cancer cells

Maj

Kokocha

Bajek

et al. 2018

Sci Rep

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“…Nonetheless, there was hypertrophy of the constructed urothelium which could lead to outlet obstruction [82]. In a recent study from 2017, unseeded and seeded cystoplasty collagen grafts were compared, with inconsistent results regarding the ingrowth of urothelial and smooth muscle cells [83].…”

Section: Clinical Presentation and Issuesmentioning

confidence: 99%

“…[82]–Collagen–fibrin scaffold and IGF-1Possible outlet obstruction because of tissue hypertrophy2017Leonhäuser et al. [83]MinipigsUnseeded and seeded collagen scaffoldsInconsistent cell ingrowth, risk of leakage…”

Section: Clinical Presentation and Issuesmentioning

confidence: 99%

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Bladder augmentation in children: current problems and experimental strategies for reconstruction

Langer

Radtke

Györi

et al. 2018

Wien Med Wochenschr

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Bladder augmentation is a demanding surgical procedure and exclusively offered for selected children and has only a small spectrum of indications. Paediatric bladder voiding dysfunction occurs either on a basis of neurological dysfunction caused by congenital neural tube defects or on a basis of rare congenital anatomic malformations. Neurogenic bladder dysfunction often responds well to a combination of specific drugs and/or intermittent self-catheterization. However, selected patients with spinal dysraphism and children with congenital malformations like bladder exstrophy and resulting small bladder capacity might require bladder augmentation. Ileocystoplasty is the preferred method of bladder augmentation to date. Because of the substantial long-and short-term morbidity of augmentation cystoplasty, recent studies have tried to incorporate new techniques and technologies, such as the use of biomaterials to overcome or reduce the adverse effects. In this regard, homografts and allografts have been implemented in bladder augmentation with varying results, but recent studies have shown promising data in terms of proliferation of urothelium and muscle cells by using biological silk grafts.

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“…Tissue engineering techniques involve a combination of cells, engineering, and materials to overcome these complications in bladder reconstruction. Naturally derived biomaterials, including porcine small intestinal submucosa, bladder acellular matrix, amniotic membrane and others have been studied for their effectiveness as scaffolds for bladder augmentation (9)(10)(11)(12). Hybrid biomaterials, using combinations of naturally derived scaffolds and synthetic polymers have also been explored to create novel biomaterials for regenerative medicine (13,14).…”

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confidence: 99%

Urinary bladder reconstruction using autologous collagenous connective tissue membrane “Biosheet” induced by in-body tissue architecture

Iimori

Naito

Iwai

et al. 2019

Preprint

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Background: Tissue engineering technology has the potential for bladder reconstruction without complications. We have previously developed autologous collagenous prosthetic tissues using in-body tissue architecture (iBTA). This is a cell-free tissue engineering technology that can produce autologous implantable tissues be a desired shape by simple subcutaneous embedding of a specially designed mold. Grafts formed by iBTA can be made in any shape and form, including sheet-shaped tissues (Biosheet). In this study, we evaluated the efficacy and safety of autologous Biosheet as bladder repair material in a canine bladder defect model. Methods: We studied four healthy adult beagles (1-2 years old, 9.3-9.9 kg). Autologous Biosheets were prepared by embedding specially designed molds into subcutaneous pouches in the beagles. Eight weeks after implantation, the molds were extracted, and collagenous connective tissues surrounding the molds were harvested as autologous Biosheet. The urinary bladder wall was excised (2 cm × 2 cm) and autologous Biosheets were sutured to the cut edge of the native bladder using a simple continuous suture pattern. The efficacy of implantation of the Biosheets was evaluated by physical examination, blood tests, abdominal ultrasound, urinalysis, and urography, at 0, 1, 3, 7, 14, 28, 56, and 84 days after the implantation. The Biosheets were extracted at 28 days (n=1) and 84 days (n=3) after implantation. Results: No side-effects were observed during follow-up. No disruption of the sheet or any urinary leakage into the peritoneal cavity was observed. Histological analysis revealed α-SMA-positive muscle fibers at the margin of the Biosheets, indicating regeneration of the urinary bladder tissue. Conclusion: This is the first report evaluating the efficacy and safety of iBTA-induced autologous “Biosheets” as a bladder repair material in a canine model. In summary, autologous Biosheets could be useful biomaterials for urological reconstruction.

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Two differentially structured collagen scaffolds for potential urinary bladder augmentation: proof of concept study in a Göttingen minipig model

Cited by 19 publications

References 42 publications

The interplay between adipose-derived stem cells and bladder cancer cells

The interplay between adipose-derived stem cells and bladder cancer cells

Bladder augmentation in children: current problems and experimental strategies for reconstruction

Urinary bladder reconstruction using autologous collagenous connective tissue membrane “Biosheet” induced by in-body tissue architecture

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