Two-dimensional agnor evaluation as a prognostic variable in urinary bladder carcinoma: A different approach via total agnor area/nucleus area per cell

Eröz

Biotechnic & Histochemistry

et al. 2014

Fine needle aspiration biopsy (FNAB) of the thyroid gland is an important tool for preoperative diagnosis; however, its benefit is limited for follicular lesions. Nucleolar organizer regions (NORs) are ribosomal gene regions that stain with silver (Ag) when they are active. These regions can be used to differentiate neoplastic and non-neoplastic lesions. We used a new AgNOR technique to investigate FNAB of cases diagnosed as follicular adenoma and carcinoma. Fourteen cases of follicular thyroid carcinoma (FTC) and 28 cases of thyroid follicular adenomas (FA) were stained using the silver NOR-associated protein (AgNOR) technique. One hundred nuclei per sample were examined, AgNORs were counted, and the total AgNOR area/nuclear area (TNORa/Na) ratio of each cell was calculated. We found that cases with FTC had significantly higher TNORa/Na than cases of FA. Also, cases with FTC had significantly higher AgNOR counts than cases with FA. AgNOR counting may help discriminate FTC and FA by routine cytopathology before surgery.

Section: Discussionmentioning

confidence: 99%

“…Analysis of interphase AgNOR proteins is widely used to differentiate neoplastic from nonneoplastic lesions (Kuno et al 2006). The detection of AgNOR proteins is an indicator of cellular proliferative activity in many types of cancers (Shiina et al 1996, Cucer et al 2007, Eroz et al 2010, 2011, 2013a.…”

mentioning

confidence: 99%

Argyrophilic nucleolar organizing region associated protein synthesis for cytologic discrimination of follicular thyroid lesions

Eröz

Biotechnic & Histochemistry

et al. 2014

“…In these studies, a significant correlation between both the mAgNOR and the pAgNOR and the quantitative result of second-look laparotomy was postulated, and the higher number of AgNORs resulted in better response to adjuvant chemotherapy [33]. It is noteworthy that early relapses occur in tumors with high proliferative activity after remission, and generally the prognosis for these patients is worse compared to patients with lower proliferative activity [15, 17, 24, 34]. Analyzing long-term treatment results, Muso et al .…”

Section: Discussionmentioning

confidence: 99%

“…There are some reports correlating AgNOR count and survival in patients suffering from cancers of non-ovarian origin [15, 17, 24, 34]. Chiusa et al ., after analysis of 65 cases of prostate cancer in a 3-year follow-up, found patients with G2 prostate cancers and high AgNOR counts to have worse prognosis (similar to G3 cancers) when compared to G2 tumors with low AgNOR counts (similar to G1 cancers) [17].…”

Section: Discussionmentioning

confidence: 99%

The AgNORs count in predicting long-term survival in serous ovarian cancer

Gottwald¹,

Danilewicz²,

Fendler³

et al. 2014

aoms

IntroductionThe value of argyrophilic nucleolar organizer regions (AgNORs) to predict survival in patients with ovarian cancer has not been clearly explained yet. The aim of study was to assess the value of analysis of the mean number of AgNORs per nucleus (mAgNOR) and mean percentage of nuclei with five or more AgNORs per nucleus (pAgNOR) in the prediction of disease-free survival (DFS) and overall survival (OS) in patients with serous ovarian cancer.Material and methodsThe study examined 52 patients treated for serous ovarian cancer with a follow-up period of 2-143 months. After silver staining paraffin specimens from primary surgery, mAgNOR and pAgNOR in cancer cells were counted and analyzed. Age, grading, radicality of surgery and FIGO staging were analyzed as covariates.ResultsMean mAgNOR equaled 4.4 ±0.9 and pAgNOR equaled 42.2 ±20.8%. Both mAgNOR and pAgNOR were the lowest in G1 tumors. The mAgNOR and pAgNOR were lower in stage I than stage IV cancers. The DFS and OS rates were respectively 15.4% and 21.2%. In univariate analysis FIGO staging, grading, and pAgNOR were associated with worse prognosis, while radicality of surgery remained a significant protective factor in terms of DFS. Higher FIGO staging and older age worsened OS. In multivariate analysis FIGO staging remained significantly associated with both DFS (HR 1.98; 95% CI 1.05-3.71) and OS (HR 1.76; 95% CI 1.00-3.10), while age affected OS rates (HR 1.78; 95% CI 1.04-2.95).ConclusionsmAgNOR and pAgNOR are useful markers of cellular kinetics. Prospective studies in larger populations are needed to confirm these results in terms of AgNORs’ effects on survival.

“…Due to their silver affi nity, these proteins are called as argyrophilic NOR (AgNOR)-associated proteins and silver staining method is the most confident to indicate nucleoli in interphase nuclei (12) and to identify active NOR-bearing chromosomes at metaphase (13). There are various studies about the importance of the interphase quantity of AgNOR in tumor pathology, for the prognostic and diagnostic description of different cancer types (14,15). To the best of our knowledge, no researches about the association between AgNOR proteins amount and the effects of curcumin treatment have been performed on EAC in literature.…”

Section: Introductionmentioning

confidence: 99%

The detection of curcumins’ antitumoral effects via argyrophilic nucleolar organizing region-associated protein synthesis in mice with ehrlich’s ascitic carcinoma

Nisari¹,

Yılmaz²,

Eröz³

et al. 2017

BLL

BACKGROUND: Curcumin is a polyphenol compound that has antioxidant, anticancer, anti-infl ammatory, antihyperlipidemic and antimicrobial effects. Nucleolar-organizing regions are the sites of the gene on chromosomes. The present study was aimed to show the antitumoral effect of curcumin via AgNOR protein synthesis in Ehrlich's ascitic carcinoma (EAC) bearing mice. METHODS: Twenty three mice with EAC were randomly divided into 3 groups as positive control (n = 7), group 2 (n = 8) and 3 (n = 8) treated intraperitoneally with curcumin (25 mg/kg) and (50 mg/kg), respectively. The animals were sacrifi ced on Day 16, the solid tumors were removed out. Then, total AgNOR area/nuclear area (TAA/NA) and the mean AgNOR number were estimated for each mice. RESULT: Statistically signifi cant differences were determined among the whole groups for TAA/NA ratio (p = 0.000), conversely mean AgNOR number (p = 0.361). When comparingthe two groups; while no difference was determined between the control and curcumin (25 mg/kg) groups (p = 0.061), the signifi cant differences were detected between the control and curcumin (50 mg/kg) groups (p = 0.000) and between curcumin (25 mg/kg) and curcumin (50 mg/kg) groups (p = 0.000) for TAA/NA ratio. However, there was no signifi cant difference for the mean AgNOR number in double comparison of the groups. CONCLUSIONS: The current study showed that curcumin had a crucial function against cancer development. Also, both AgNOR values might be used as biomarkers for detection of the most reliable therapeutic dose selection of cancer treatment (Tab. 3, Fig. 2, Ref. 27). Text in PDF www.elis.sk.