2019
DOI: 10.1007/s11340-019-00473-8
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Two-Dimensional Culture Systems to Enable Mechanics-Based Assays for Stem Cell-Derived Cardiomyocytes

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Cited by 13 publications
(20 citation statements)
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“…In addition to providing a physiologically relevant stiffness, the compliant PDMS used here enabled noninvasive, live imaging mechanical outputs through the use of bright field imaging and subsequent digital image correlation analysis. As reported in our prior work (Notbohm et al, 2019), hPSC‐CMs seeded on 5 and 10 kPa generated more contractile strain than those seeded on 50 kPa substrates. This is in good agreement with previous studies identifying an optimal stiffness for maximum contractile output (Engler et al, 2008; Jacot et al, 2008; Ribeiro et al, 2015).…”
Section: Discussionsupporting
confidence: 82%
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“…In addition to providing a physiologically relevant stiffness, the compliant PDMS used here enabled noninvasive, live imaging mechanical outputs through the use of bright field imaging and subsequent digital image correlation analysis. As reported in our prior work (Notbohm et al, 2019), hPSC‐CMs seeded on 5 and 10 kPa generated more contractile strain than those seeded on 50 kPa substrates. This is in good agreement with previous studies identifying an optimal stiffness for maximum contractile output (Engler et al, 2008; Jacot et al, 2008; Ribeiro et al, 2015).…”
Section: Discussionsupporting
confidence: 82%
“…Purified hiPSC‐CMs were seeded onto a 10kPa PDMS substrate patterned with Matrigel lanes of varying widths (Figure 1a,b). As previously reported, micropattern lane width influences the morphology and myofibril alignment of hiPSC‐CMs with smaller lane widths displaying more organization compared to larger lane widths (Napiwocki et al, 2017; Notbohm et al, 2019; Salick et al, 2014). A lane width of 30 µm was selected for the patterned constructs because it yields 1–2 hiPSC‐CMs in the width of the features and maintains alignment of myofibrils.…”
Section: Resultsmentioning
confidence: 58%
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