Two-dimensional DOSY experiment with Excitation Sculpting water suppression for the analysis of natural and biological media

Interfacial Partitioning of a Loop Hinge Residue Contributes to Diacylglycerol Affinity of Conserved Region 1 Domains

“…The total detergent concentration in experiments (i) and (iii) was converted to micelle concentration using an aggregation number of 56. The aggregation number was calculated from the translational diffusion coefficients that we measured in a set of pulsed-field gradient NMR experiments (43) and is consistent with previous findings (44). The binding curves were constructed by plotting the residue-specific chemical shift changes ⌬ against the micelle concentration in (i) and (iii), and the total DPS concentration in (ii).…”

Chromatographic NMR with size exclusion chromatography stationary phases

“…Typically 32 transients were recorded per gradient increment, over a spectral width of 8 kHz. Solvent suppression was performed using the excitation sculpting approach [33,34]. The spectra were processed using the DOSY Toolbox [35] with mono-exponential fitting of the Stejskal-Tanner equation [2] to the observed echo attenuation, appropriately modified for the Oneshot sequence [32].…”

Advances in NMR-based biofluid analysis and metabolite profiling

“…The pursuit for optimal solvent suppression has continued over the past several decades. There are two basic concepts in water suppression: (1) signals of interest (for example, metabolites) can be selected by following the desired coherence transfer pathway, examples include WATERGATE-type solvent suppression including excitation sculpting; 56 (2) the interesting magnetization can be stored along the z -axis while the solvent is suppressed, examples include weak RF irradiation type methods such as Pre-SAT, 1D NOESY, Presaturation utilizing relaxation gradients and echoes (PURGE) 57 and combinations of gradient and weak RF pulses such as the WET sequence. Several of these sequences have been compared.…”