Two-dimensional gel electrophoresis in peritoneal fluid samples identifies differential protein regulation in patients suffering from peritoneal or ovarian endometriosis

Wölfler, M; Meinhold‐Heerlein, Ivo; Söhngen, Linda; Rath, Werner; Knüchel, Ruth; Neulen, Joseph; Maass, Nicolaì; Henkel, Corinna

doi:10.1016/j.fertnstert.2011.03.061

Cited by 18 publications

(13 citation statements)

References 26 publications

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“…Numerous studies have been conducted on endometrial tissue and secretions, endometrial receptivity, ovaries, uterine fluid, follicular fluid, peritoneal fluid, plasma, endometriotic tissue, and tissues from PCOS patients [8, 13, 31, 46–48]. However, the collection of reproductive samples is a challenge as some of the samples have to be obtained invasively [44].…”

Section: Power Of Proteomics In Studies Of Female Infertilitymentioning

confidence: 99%

“…Studies have shown that oxidative stress causes alterations in certain protein pathways and the abnormal expression of several proteins could possibly lead to the pathophysiology of female infertility. The abnormally expressed proteins could be integral factors responsible for oxidative stress reaction, or antioxidants or different binding proteins that may bind oxidants and antioxidants such as superoxide dismutase (SOD), paraoxonase (PON), hemopexin, apolipoproteins, and heat shock proteins [8–13]. …”

Section: Introductionmentioning

confidence: 99%

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Power of Proteomics in Linking Oxidative Stress and Female Infertility

Gupta

Ghulmiyyah

Sharma

et al. 2014

BioMed Research International

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Endometriosis, PCOS, and unexplained infertility are currently the most common diseases rendering large numbers of women infertile worldwide. Oxidative stress, due to its deleterious effects on proteins and nucleic acids, is postulated to be the one of the important mechanistic pathways in differential expression of proteins and in these diseases. The emerging field of proteomics has allowed identification of proteins involved in cell cycle, as antioxidants, extracellular matrix (ECM), cytoskeleton, and their linkage to oxidative stress in female infertility related diseases. The aim of this paper is to assess the association of oxidative stress and protein expression in the reproductive microenvironments such as endometrial fluid, peritoneal fluid, and follicular fluid, as well as reproductive tissues and serum. The review also highlights the literature that proposes the use of the fertility related proteins as potential biomarkers for noninvasive and early diagnosis of the aforementioned diseases rather than utilizing the more invasive methods used currently. The review will highlight the power of proteomic profiles identified in infertility related disease conditions and their linkage with underlying oxidative stress. The power of proteomics will be reviewed with regard to eliciting molecular mechanisms for early detection and management of these infertility related conditions.

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Section: Power Of Proteomics In Studies Of Female Infertilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Power of Proteomics in Linking Oxidative Stress and Female Infertility

Gupta

Ghulmiyyah

Sharma

et al. 2014

BioMed Research International

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“…Another work suggested that the administration of gonadotropin-releasing hormone analog (GnRH-a) may reduce the inflammation in the peritoneal cavity by determining the changes in the peritoneal fluid proteome of women with endometriosis and under GnRH-a treatment [26]. Differential protein expression in peritoneal fluid of patients with ovarian or peritoneal endometriosis was used to reveal the local dysregulation potentially involved in the pathogenesis [27]. Recently, peritoneal fluid was used for identifying potential diagnostic biomarkers for ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Impact of uremic environment on peritoneum: A proteomic view

Wang¹,

Lin²,

Chien³

et al. 2012

Journal of Proteomics

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“…Another study used the same experimental approach to identify 11 proteins that were differentially expressed in PF that was collected from endometriosis patients or control individuals; 8 were implicated in endometriosis pathogenesis [12]. The same group used an ELISA approach to confirm downregulation of one of the differentially expressed proteins, hemopexin, in a larger set of case and control PF samples [45].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the signals of interest are often from a point source that is distal to where the sample is collected, thereby creating the additional problem of dilution [11]. These difficulties have prompted analyses of more proximal samples, such as peritoneal fluid from women with endometriosis [12-14] or the actual cell type of interest such as fat from obese individuals [15]. …”

Section: Introductionmentioning

confidence: 99%

Urine, peritoneal fluid and omental fat proteomes of reproductive age women: Endometriosis-related changes and associations with endocrine disrupting chemicals

Williams

Miroshnychenko

Johansen

et al. 2015

Journal of Proteomics

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Endometriosis, ectopic growth of the uterine lining (endometrium), which affects 6–11% of reproductive age women, is associated with pelvic pain and infertility. We investigated the peritoneal fluid (PF), urine and omental fat (OF) proteomes of women with endometriosis vs. individuals with no surgically visualized endometriosis. All participants were enrolled in the NICHD-funded ENDO Study. A two-step proteomic study was performed. The first, a broad survey, employed a semi-quantitative gel LC-mass spectrometry (MS) workflow: SDS PAGE fractionation, trypsin digestion and LC–MS/MS. The results showed sample integrity but failed to detect any differences between women with and without endometriosis. The second step was a quantitative analysis of OF samples. We employed another sample set (n = 30) from women ± disease and isobaric mass-tag (iTRAQ) chemistry to label peptides and 2D LC–MS/MS for protein identification and quantification. Three proteins—matrix metalloproteinase-9, neutrophil elastase, and FAM49B—were significantly lower in abundance in samples from women with endometriosis. Interestingly, neutrophil elastase and FAM49B levels were associated with higher levels of a subset of endocrine disrupting chemicals (EDCs) that were previously measured in the same samples. The results of these experiments showed the feasibility of associating endometriosis with changes in the OF protein repertoire and EDC levels. Biological significance Endometriosis, pathological growth of the uterine lining, is associated with significant morbidities, including pain and infertility. However, the causes of this common condition are poorly understood. This study determined whether endometriosis was associated with changes in the protein composition of peritoneal fluid, urine and/or omental fat. A protein of unknown function (FAM49B) and two proteinases (metalloproteinase-9, neutrophil elastase) were down regulated in OF samples from women with versus without endometriosis. These findings suggested proteinase imbalances at sites that were distant from the endometriotic lesions. Additionally, FAM49B and neutrophil elastase levels were associated with higher levels of a subset of environmental chemicals that were quantified in the same samples, suggesting other possible associations. Thus, this work generated hypotheses that will be tested in further studies.

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Two-dimensional gel electrophoresis in peritoneal fluid samples identifies differential protein regulation in patients suffering from peritoneal or ovarian endometriosis

Cited by 18 publications

References 26 publications

Power of Proteomics in Linking Oxidative Stress and Female Infertility

Power of Proteomics in Linking Oxidative Stress and Female Infertility

Impact of uremic environment on peritoneum: A proteomic view

Urine, peritoneal fluid and omental fat proteomes of reproductive age women: Endometriosis-related changes and associations with endocrine disrupting chemicals

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