Two-dimensional gel electrophoretic protein patterns in high-grade human astrocytomas

Narayan, Raj K.; Heydorn, William E.; Creed, G. Joseph; Kornblith, Paul L.; Jacobowitz, David M.

doi:10.1007/978-1-4613-2297-9_2

Cited by 5 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two-dimensional gel electrophoresis was performed to examine the cultures and confirm their glial cell origin. A characteristic pattern of GFAP was observed (22, 24) (Fig. 3), which was blocked when samples were preincubated with anti-GFAP antibody (data not shown).…”

Section: Isolation Of Thrombospondin From [35sjmethionine-labeledmentioning

confidence: 87%

See 1 more Smart Citation

Human brain glial cells synthesize thrombospondin.

Asch¹,

Leung²,

Shapiro³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

102

View full text Add to dashboard Cite

Section: Isolation Of Thrombospondin From [35sjmethionine-labeledmentioning

confidence: 87%

“…No antibiotics were used and the cell line was monitored for mycoplasma by the method of Chen (20). Two (24). After electrophoresis, gels were stained with Coomassie blue R-250, destained, dried, and autoradiographed.…”

Section: Methodsmentioning

confidence: 99%

Human brain glial cells synthesize thrombospondin.

Asch¹,

Leung²,

Shapiro³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

102

View full text Add to dashboard Cite

“…But it is evident that those factors may be responsible for proliferation in general rather than for a cell type-specific hyperproliferation. Narayan and coworkers used 2-D PAGE to describe the protein pattern of astrocytomas, ependymomas and medulloblastomas but they failed to describe any tumor marker in detail [40,41]. One great disadvantage of their strategy is that they used crude sample preparations obtained from punch biopsies.…”

Section: Discussionmentioning

confidence: 99%

Nuclear and DNA‐binding proteins in human brain tumors

1991

View full text Add to dashboard Cite

Nuclear proteins obtained from human brain tumor cell lines by differential salt extraction were subjected to high-resolution two-dimensional electrophoresis. Several hundred spots were detectable in the low salt (0.4 M NaCl) extract using silver staining. These patterns exhibited remarkable differences between the different cell lines we analyzed. A less complex pattern occurred when nuclei were subsequently treated with high salt (2.5 M NaCl/5 M urea). We compared the electropherograms from various human glioblastoma cell lines and found them very similar and even a high degree of similarity occurs between glioblastomas and other human tumor cell lines. Beside these more general observations we detected several proteins at least enriched in human glioblastomas which were totally absent in low grade astrocytomas and nonglial tumors. They could be separated from the bulk of nonspecific proteins by simple modifications of the isoelectric focusing conditions. From these results we conclude that nuclear proteins obtained by sequential salt extraction and separated by two-dimensional techniques may provide tumor specific proteins suitable for antibody production.

show abstract

“…Most studies agree that single-photon emission computed tomography (SPECT) improves image contrast and resolution, as compared to planar scanning (27). However, this can also increase the rate of false-positive results, since reconstruction artifacts can occur with SPECT.…”

Section: Radioimmunodetection (Raid)mentioning

confidence: 99%

“…Four antibodies recognized linear sites. Antibody 2 recognized one linear site, VDELD (38-42); while antibodies 5, 13, and 14 each recognized the same two sites, YGCYCGPGG (25)(26)(27)(28)(29)(30)(31)(32)(33) and FPKL TL YSW (61-69) ( Table 1). The YGCYCGXGG is a highly conserved region in all PLA2s important in the binding of Ca++(10).…”

Section: Epitope Mapping Of Monoclonal Antibodiesmentioning

confidence: 99%

Immunobiology of Proteins and Peptides VI

1991

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

A Continuation Order Plan is available for this series. A continuation order will bring delivery of each new volume immediately upon publication. Volumes are billed only upon actual shipment. For further information please contact the publisher. 91-S18S7 CIPThe articles in this volume represent papers delivered by invited speakers at the 6th International Symposium on the Immunobiology of Proteins and Peptides. In addition, a few of the abstracts submitted by participants were scheduled for minisymposia and some of the authors, whose presentations were judged by the Scientific Council to be of high quality, were invited to submit papers for publication in this volume.This symposium was established in 1976 for the purpose of bringing together, once every two or three years, active investigators in the forefront of contemporary immunology, to present their findings and discuss t heir significance in the light of current concepts and to identify important new directions of investigation. The founding of the symposium was stimulated by the achievement of major breakthroughs in the understanding of the immune recognition of proteins and peptides. We believed that these breakthroughs will lead to the creation of a new generation of peptide reagents which should have enormous potential in biological, therapeutic and basic applications. This anticipated explosion has in fact since occurred and many applications of these pep tides are now being realized.The sixth symposium was devoted to four major areas: Human immunodeficiency virus, antibody immunoconjugates, bacterial vaccines and immunomodulators. In this volume, many important papers will deal with various aspects of structure and biology of HIV and SIV, the expression and regulation of their genes and the immunology of their envelope proteins. Antibody immunoconjugates have become an important tool for specific targeting of drugs and radioisotopes in chemotherapy and radio immunotherapy of certain malignancies. Papers by several leading investigators deal here with recent advances in this important field. Manipulation of the immune system by immunodulators, or by other strategies, is perhaps one of the most promising applications of the advances in immunology to disease therapy. Many important papers deal with designs and applications of vaccines against selected bacterial agents. To achieve an intelligent effective design of a vaccine, it is crucial to know details of the humoral and cellular immune responses against the infectious organism. How to maximize a required antibody or T-cell response or to reduce its magnitude is often desired in the design of an immunological defense strategy. What is the best means of delivery? How can tolerance be achieved in certain cases? These and other important questions that need to be appreciated in the design of vaccines are discussed in this volume.Finally, I should like to express, on behalf of the organization, our gratitude to our sponsors whose generous support made this conference possible.

show abstract

Two-dimensional gel electrophoretic protein patterns in high-grade human astrocytomas

Cited by 5 publications

References 36 publications

Human brain glial cells synthesize thrombospondin.

Human brain glial cells synthesize thrombospondin.

Nuclear and DNA‐binding proteins in human brain tumors

Immunobiology of Proteins and Peptides VI

Contact Info

Product

Resources

About