Two-dimensional high performance liquid chromatography for determination of homocysteine, methionine and cysteine enantiomers in human serum

Deáková, Zuzana; Ďuračková, Zdeňka; Armstrong, Daniel W.; Lehotay, J.

doi:10.1016/j.chroma.2015.07.009

Cited by 54 publications

(20 citation statements)

References 53 publications

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“…3 Therefore, the development of a method for detecting L-cysteine with high sensitivity and selectivity is necessary from a clinical point of view. At present, some techniques, such as HPLC, 4 uorescent methods, 5 spectrophotometric methods 6 and chemiluminescence 7 have already been developed to detect Lcysteine in biological and pharmaceutical samples, but many of the mentioned methods are either complicated or lacking in selectivity. Compared with other methods, electrochemical method shows many advantages, such as simple operation, inexpensive consumption, good selectivity and convenient realtime determination.…”

Section: Introductionmentioning

confidence: 99%

Simple synthesis of ZnO nanoparticles on N-doped reduced graphene oxide for the electrocatalytic sensing ofl-cysteine

Yang

et al. 2017

RSC Adv.

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A new kind of ZnO nanoparticle/N-doped reduced graphene oxide nanocomposite (ZnONPs/N-rGO) was synthesized through a low temperature, low-cost and one step hydrothermal process. By using the prepared nanocomposite as a modified electrode material, an L-cysteine electrochemical sensor was fabricated. X-ray diffraction, transmission electron microscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and electrochemical techniques were used to study the nanocomposite.The nanocomposite shows a high electro-catalytic activity toward L-cysteine oxidation. Under the optimum determination conditions, the nanocomposite modified electrode provided L-cysteine with a broad detection range of 0.1-705.0 mM and a limit of detection of 0.1 mM (S/N ¼ 3). This novel method was successfully used to determine L-cysteine in real samples.

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Section: Introductionmentioning

confidence: 99%

Simple synthesis of ZnO nanoparticles on N-doped reduced graphene oxide for the electrocatalytic sensing ofl-cysteine

Yang

et al. 2017

RSC Adv.

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“…Lehotay's research group reported a two-dimensional HPLC-ECD method for determination of the enantiomers of Hcy, Cys, and Met using a combination of ODS and teicoplanin aglycone columns [69,70]. The chiral separation of Hcy, Cys, and Met enantiomers was realized in a single 130 min analytical run.…”

Section: Electrochemical Detectionmentioning

confidence: 99%

HPLC Analysis of Homocysteine and Related Compounds

Wada¹,

Nakamura²,

Nakashima³

2018

Non-Proteinogenic Amino Acids

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Homocysteine (Hcy), a sulfur-containing amino acid, is a representative intermediate metabolite of methionine (Met) to cysteine (Cys) via several intermediates. An elevated level of Hcy in plasma plays an important role in diseases such as neural tube defects and Down syndrome. Homocystinuria is the most common inborn error of sulfur metabolism and is caused by mutations in the metabolic enzymes of Hcy. These errors can be caused by abnormal levels of Met metabolites and classified on the basis of plasma Met levels. Additionally, Hcy and related compounds such as glutathione play an important role in maintaining homeostasis. Therefore, the simultaneous determination of Hcy and/or related compounds is required for appropriate clinical management of several diseases. The sulfur-containing amino acids and their derivatives in biological samples are quantified sensitively using high-performance liquid chromatography methods coupled with various detection methods such as UV/Vis, fluorescence, chemiluminescence, electrochemical, mass spectrometry, and tandem mass spectrometry. In this chapter, we review recent advances in these analytical methods and their applications.

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“…Homocysteine (Hcy) is a thiol containing non‐protein amino acid involved in methionine´s metabolism that is considered a biomarker of cardiovascular and neurodegenerative diseases . Few works reported the chiral separation of Hcy by HPLC and only two works employed CE for this purpose . The former relied on the use of a high concentration of γ‐CD (50 mM) to obtain the baseline separation of Hcy enantiomers derivatized with 4‐aminosulfonyl‐7‐fluoro‐2,1,3‐benzoxadiazole in long analysis times (up to 40 min) whereas the latter, performed by our research group, was based on the synergistic effect originated by the use of a dual system of γ‐CD and the CIL (R)‐N,N,N‐trimethyl‐2‐aminobutanol‐bis(trifluoromethanesulfon)imidate (EtCholNTf 2 ).…”

Section: Introductionmentioning

confidence: 99%

Nuclear magnetic resonance to study the interactions acting in the enantiomeric separation of homocysteine by capillary electrophoresis with a dual system of γ‐cyclodextrin and the chiral ionic liquid EtCholNTf₂

et al. 2019

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The enantiomeric separation of 9‐fluorenylmethoxycarbonyl chloride (FMOC)‐homocysteine (Hcy) by CE was investigated using γ‐CD and the chiral ionic liquid (R)‐(1‐hydroxybutan‐2‐yl)(trimethyl)azanium‐bis(trifluoromethanesulfon)imidate (also called (R)‐N,N,N‐trimethyl‐2‐aminobutanol‐bis(trifluoromethane‐sulfon)imidate) (EtCholNTf2) as chiral selectors. Using 2 mM γ‐CD and 5 mM EtCholNTf2 in 50 mM borate buffer (pH 9), FMOC‐Hcy enantiomers were separated with a resolution value of 3.8. A reversal in the enantiomer migration order in comparison with the single use of γ‐CD in the separation buffer was obtained. Then, NMR experiments were carried out to elucidate the interactions taking place in the enantiomeric separation of FMOC‐Hcy. NMR analyses highlighted the formation of an inclusion complex since the hydrophobic group of FMOC‐Hcy was inserted into the γ‐CD cavity. Moreover, interactions between EtCholNTf2 and γ‐CD were also observed, suggesting that the chiral ionic liquid would also enter the cavity of the γ‐CD.

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Two-dimensional high performance liquid chromatography for determination of homocysteine, methionine and cysteine enantiomers in human serum

Cited by 54 publications

References 53 publications

Simple synthesis of ZnO nanoparticles on N-doped reduced graphene oxide for the electrocatalytic sensing ofl-cysteine

Simple synthesis of ZnO nanoparticles on N-doped reduced graphene oxide for the electrocatalytic sensing ofl-cysteine

HPLC Analysis of Homocysteine and Related Compounds

Nuclear magnetic resonance to study the interactions acting in the enantiomeric separation of homocysteine by capillary electrophoresis with a dual system of γ‐cyclodextrin and the chiral ionic liquid EtCholNTf₂

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Two-dimensional high performance liquid chromatography for determination of homocysteine, methionine and cysteine enantiomers in human serum

Cited by 54 publications

References 53 publications

Simple synthesis of ZnO nanoparticles on N-doped reduced graphene oxide for the electrocatalytic sensing ofl-cysteine

Simple synthesis of ZnO nanoparticles on N-doped reduced graphene oxide for the electrocatalytic sensing ofl-cysteine

HPLC Analysis of Homocysteine and Related Compounds

Nuclear magnetic resonance to study the interactions acting in the enantiomeric separation of homocysteine by capillary electrophoresis with a dual system of γ‐cyclodextrin and the chiral ionic liquid EtCholNTf2

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Nuclear magnetic resonance to study the interactions acting in the enantiomeric separation of homocysteine by capillary electrophoresis with a dual system of γ‐cyclodextrin and the chiral ionic liquid EtCholNTf₂