Two-dimensional nanosecond electric field mapping based on cell electropermeabilization

Chen, Meng-Tse; Jiang, Chunqi; Vernier, P. Thomas; Wu, Yu‐Hsuan; Gundersen, M.A.

doi:10.1186/1757-5036-2-9

Cited by 23 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, we considered that the electric field itself may be saturating in the solution. However, a number of studies, using similar electric pulse exposure systems, have shown a proportional increase in uptake of markers of membrane permeabilization, such as propidium ion and Yo-Pro-1, on applied voltage at amplitudes far beyond those used in this study (24,28,29). The electric-field dependence was also corroborated by whole-cell patchclamp experiments in which the membrane resistance was reduced proportional to the applied energy to the system (30,31).…”

Section: Comparison Of Experimental and Model Resultssupporting

confidence: 72%

Visualization of Dynamic Sub-microsecond Changes in Membrane Potential

Beier

Roth

Bixler

et al. 2019

Biophysical Journal

View full text Add to dashboard Cite

Direct observation of rapid membrane potential changes is critical to understand how complex neurological systems function. This knowledge is especially important when stimulation is achieved through an external stimulus meant to mimic a naturally occurring process. To enable exploration of this dynamic space, we developed an all-optical method for observing rapid changes in membrane potential at temporal resolutions of $25 ns. By applying a single 600-ns electric pulse, we observed sub-microsecond, continuous membrane charging and discharging dynamics. Close agreement between the acquired results and an analytical membrane-charging model validates the utility of this technique. This tool will deepen our understanding of the role of membrane potential dynamics in the regulation of many biological and chemical processes within living systems.

show abstract

Section: Comparison Of Experimental and Model Resultssupporting

confidence: 72%

Visualization of Dynamic Sub-microsecond Changes in Membrane Potential

Beier

Roth

Bixler

et al. 2019

Biophysical Journal

View full text Add to dashboard Cite

show abstract

“…Therefore, a classical one, like the yo-pro-1 iodide 19,54 , was used to compare the PM permeabilization thresholds between the two markers (Supplementary Fig. S2).…”

Section: Discussionmentioning

confidence: 99%

Electropermeabilization of Inner and Outer Cell Membranes with Microsecond Pulsed Electric Fields: Quantitative Study with Calcium Ions

Hanna

Denzi

Liberti

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Microsecond pulsed electric fields (μsPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this technology is complex to use. Here we report that the endoplasmic reticulum (ER) membrane can also be electropermeabilized by one 100 µs pulse without affecting the cell viability. Indeed, using Ca2+ as a permeabilization marker, we observed cytosolic Ca2+ peaks in two different cell types after one 100 µs pulse in a medium without Ca2+. Thapsigargin abolished these Ca2+ peaks demonstrating that the calcium is released from the ER. Moreover, IP3R and RyR inhibitors did not modify these peaks showing that they are due to the electropermeabilization of the ER membrane and not to ER Ca2+ channels activation. Finally, the comparison of the two cell types suggests that the PM and the ER permeabilization thresholds are affected by the sizes of the cell and the ER. In conclusion, this study demonstrates that µsPEF, which are easier to control than nsPEF, can permeabilize internal membranes. Besides, μsPEF interaction with either the PM or ER, can be an efficient tool to modulate the cytosolic calcium concentration and study Ca2+ roles in cell physiology.

show abstract

“…for the conductivity and permittivity of grey matter [ 30 ] was interpolated in Mathematica in order to estimate the values at 250 kHz (Table 2 ). The electro-quasistatic assumption is validated based on the fact that the frequency of the field corresponds to a wavelength (3.5 m) and skin depth (0.5 m) that are greater than the longest dimension in the geometry [ 31 ]. The brain subdomain was modeled as a 0.75 cm × 0.75 cm × 0.425 cm ellipsoid having a total volume of 1 cm 3 [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction

et al. 2011

View full text Add to dashboard Cite

BackgroundTherapeutic irreversible electroporation (IRE) is an emerging technology for the non-thermal ablation of tumors. The technique involves delivering a series of unipolar electric pulses to permanently destabilize the plasma membrane of cancer cells through an increase in transmembrane potential, which leads to the development of a tissue lesion. Clinically, IRE requires the administration of paralytic agents to prevent muscle contractions during treatment that are associated with the delivery of electric pulses. This study shows that by applying high-frequency, bipolar bursts, muscle contractions can be eliminated during IRE without compromising the non-thermal mechanism of cell death.MethodsA combination of analytical, numerical, and experimental techniques were performed to investigate high-frequency irreversible electroporation (H-FIRE). A theoretical model for determining transmembrane potential in response to arbitrary electric fields was used to identify optimal burst frequencies and amplitudes for in vivo treatments. A finite element model for predicting thermal damage based on the electric field distribution was used to design non-thermal protocols for in vivo experiments. H-FIRE was applied to the brain of rats, and muscle contractions were quantified via accelerometers placed at the cervicothoracic junction. MRI and histological evaluation was performed post-operatively to assess ablation.ResultsNo visual or tactile evidence of muscle contraction was seen during H-FIRE at 250 kHz or 500 kHz, while all IRE protocols resulted in detectable muscle contractions at the cervicothoracic junction. H-FIRE produced ablative lesions in brain tissue that were characteristic in cellular morphology of non-thermal IRE treatments. Specifically, there was complete uniformity of tissue death within targeted areas, and a sharp transition zone was present between lesioned and normal brain.ConclusionsH-FIRE is a feasible technique for non-thermal tissue ablation that eliminates muscle contractions seen in IRE treatments performed with unipolar electric pulses. Therefore, it has the potential to be performed clinically without the administration of paralytic agents.

show abstract

Two-dimensional nanosecond electric field mapping based on cell electropermeabilization

Cited by 23 publications

References 19 publications

Visualization of Dynamic Sub-microsecond Changes in Membrane Potential

Visualization of Dynamic Sub-microsecond Changes in Membrane Potential

Electropermeabilization of Inner and Outer Cell Membranes with Microsecond Pulsed Electric Fields: Quantitative Study with Calcium Ions

High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction

Contact Info

Product

Resources

About