Two-dimensional NMR data of a water-soluble β-(1→3, 1→6)-glucan from Aureobasidium pullulans and schizophyllan from Schizophyllum commune

Kono, Hiroyuki; Kondo, Nobuhiro; Hirabayashi, Katsuki; Ogata, Makoto; Totani, Kiichiro; Ikematsu, Shinya; Osada, Motonobu

doi:10.1016/j.dib.2017.09.067

Cited by 18 publications

(9 citation statements)

References 2 publications

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“…Analysis of 13 C NMR corroborates the presence of β-glucans as the main signals observed in the spectra of Gi -MRSW and Gi -FSME fractions that are characteristic of this class of compounds [ 31 ]. Signals from the Gi -FPME fraction correspond to a heterogalactan, commonly found in macrofungi, which contains a main chain of α-Gal p (1→6)-linked, partially substituted in O-2 by non-reducing ends of β-Man p and α-Fuc p [ 32 ].…”

Section: Resultsmentioning

confidence: 80%

“…Although the 13 C NMR spectra of Gi -MRSW ( Figure 2 ) and Gi -FSME ( Figure 2 ) fractions suggest the majority presence of β-glucans in these fractions, they have distinct structures. The 13 C NMR data of Gi -MRSW showed similarities with schyzophillan-type glucan, consisting of a main chain of β-Glc p (1→3)- connected units and containing branches in O-6 by non-reducing terminals of β-Glc p , on average one branching every three units of the main chain [ 31 ]. The glycosidic linkages of the glucan were shown by the presence of 3- O -substituted signals at δ 86.61, 86.23, and 85.99, and O-substituted-CH 2 -6 signal at δ 68.46.…”

Section: Resultsmentioning

confidence: 99%

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Immunomodulatory Effect of Polysaccharides from the Mushroom-Forming Basidiomycete Gymnopilus imperialis (Agaricomycetes, Basidiomycota)

et al. 2022

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Gymnopilus consists of a widely distributed genus of basidiomycetes, especially in tropical regions of the world, such as Japan, Australia, Paraguay, and Brazil. This genus biosynthesizes interesting bioactive compounds, such as sesquiterpenoids, oligoisoprenoids, styrylpyrones, and lectins. In the present study, the aqueous extract of the basidiomata of Gymnopilus imperialis (Basidiomycota, Agaricomycetes, Agaricales, Hymenogastraceae) was obtained by using the accelerated solvent extraction (ASE) technique, followed by the precipitation of polysaccharide fraction with ethanol. Further purification by freeze-thawing processes, Fehling solution precipitation, and membrane dialysis with different pore sizes yield three main polysaccharide fractions (Gi-MRSW, Gi-PFME, and Gi-SFME). According to monosaccharide composition and 13C-NMR data, the Gi-MRSW and Gi-SFME fractions showed to be composed mainly of β-glucans and Gi-PFME by a heterogalactan. Moreover, the immunomodulatory potential of Gi-MRSW was evaluated using RAW 264.7 murine macrophage as a study model. The nitric oxide production was significantly increased in treated samples, and the expression of inducible nitric oxide synthase (iNOS) showed that the fraction Gi-MRSW from G. imperialis induces the M1 polarization phenotype.

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Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Immunomodulatory Effect of Polysaccharides from the Mushroom-Forming Basidiomycete Gymnopilus imperialis (Agaricomycetes, Basidiomycota)

et al. 2022

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“…However, the β-1,3-glucan-chitin complex results in higher C1 chemical shift values [25,26]. Sample hydration and molecular weight could impact the chemical shift with lower water content, resulting in a chemical shift toward higher values [26,40]. In the HWN fraction, higher chemical shift values could be attributed to →3)-β-Glcp-(1→ linkage and the β-linked non-reducing ends of β-Glcp-1→.…”

Section: Discussionmentioning

confidence: 99%

Solid-State 13C Nuclear Magnetic Resonance Study of Soluble and Insoluble β-Glucans Extracted from Candida lusitaniae

Bikmurzin,

Maršalka,

Kalėdienė

2023

Molecules

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β-glucans are widely known for their biological activities. However, the choice of extraction method can significantly influence their structural characteristics, thereby potentially impacting their biological functions. In this paper, three fractions of β-glucans were obtained from Candida lusitaniae yeast via alkali and hot-water extraction methods and were analyzed using solid-state 13C nuclear magnetic resonance (NMR) spectroscopy. Solid-state NMR spectroscopy was used as a nondestructive technique that preserves the structure of the analyzed molecules. The results suggest that differences in the β-glucan structure are affected by the choice of extraction method. The main difference occurred in the 82–92 ppm region with signal presence suggesting that β-glucans have a linear structure when hot-water-extracted, which is absent in alkali-extracted fractions resulting in the acquisition of β-glucans with an ordered, possibly helical structure. A hot-water extracted water-insoluble (HWN) fraction consists of linear β-1,3-glucans with other signals indicating the presence of β-1,6-linked side chains, chitin and small amounts of α-glucan impurities. For those that are alkali-extracted, alkali-insoluble (AN) and water-soluble (AWS) fractions are structurally similar and consist of an ordered β-1,3-glucan structure with β-1,6-linked side chains and a significant amount of α-glucan and chitin in both fractions.

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“…APG was kindly provided from Itochu Sugar Co. (Japan), which was prepared according to a previously reported method [[2], [3], [4]]. SPG was purchased from InvivoGen (USA).…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Solid-state relaxation NMR dataset for a water-soluble β-(1→3, 1→6)-glucan from Aureobasidium pullulans and schizophyllan from Schizophyllum commune

et al. 2020

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We report the solid-state nuclear magnetic resonance (NMR) relaxation dataset for a triple helix and a random structure of water-soluble Aureobasidium pullulans β-(1→3, 1→6)-d-glucan (APG) and those of schizophyllan from Schizophyllum commune (SPG), obtained by the Bruker BioSpin 500 MHz NMR spectrometer. These data include solid-state proton spin-lattice relaxation in the rotating frame (T1ρH) and 13C spin-lattice relaxation (T1C) of these two β-(1→3, 1→6)-glucans, which are related to the subject of article in International Journal of Biological Macromolecules, entitled “Characterization of the secondary structure and order–disorder transition of a β-(1→3, 1→6)-glucan from Aureobasidium pullulans” [1]. Data can help to investigate the structural characterization of the structural polysaccharides.

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Two-dimensional NMR data of a water-soluble β-(1→3, 1→6)-glucan from Aureobasidium pullulans and schizophyllan from Schizophyllum commune

Cited by 18 publications

References 2 publications

Immunomodulatory Effect of Polysaccharides from the Mushroom-Forming Basidiomycete Gymnopilus imperialis (Agaricomycetes, Basidiomycota)

Immunomodulatory Effect of Polysaccharides from the Mushroom-Forming Basidiomycete Gymnopilus imperialis (Agaricomycetes, Basidiomycota)

Solid-State 13C Nuclear Magnetic Resonance Study of Soluble and Insoluble β-Glucans Extracted from Candida lusitaniae

Solid-state relaxation NMR dataset for a water-soluble β-(1→3, 1→6)-glucan from Aureobasidium pullulans and schizophyllan from Schizophyllum commune

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