Two-dimensional polyacrylamide gel electrophoresis of bovine seminal plasma proteins and their relation with semen freezability

Jobim, Mariana; Oberst, Eneder Rosana; Salbego, Christianne Gazzana; Souza, Diogo O.; Wald, Vera Beatriz; Tramontina, Francine; Mattos, Rodrigo Costa

doi:10.1016/s0093-691x(03)00230-9

Cited by 104 publications

(77 citation statements)

References 48 publications

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“…Higher abnormal sperm percentage in the ejaculate reduce the fertility rate (Graham and Mocé, 2005), however, this study revealed that incidence of higher abnormal sperm morphology was one of the major concerns for poor or unacceptable freezability of semen in large number (71%) of crossbred bulls, although there are many other factors that affect the freezability of semen (Pangawkar et al, 1988;Jobim et al, 2004).…”

Section: Effect Of Age On Bulls' Spermiogrammentioning

confidence: 77%

Effect of age on spermiogram of Holstein Friesian × Sahiwal crossbred bulls

Mandal

Kumar

Tyagi

2010

Animal

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This study was conducted on 94 Frieswal (5/8 Holstein Friesian 3/8 Sahiwal) crossbred bulls of three different grades, categorized based on their semen freezability visualising Group 1 (consistently freezable semen producer bulls, N 5 11), Group 2 (inconsistent freezable, N 5 16) and Group 3 (Non freezable, N 5 67). Each group was further divided into two classes that is young (up to 30 months) and adult (31 to 70 months) bulls depending upon their age. Sperm morphology was studied by using the eosin-nigrosin staining technique. Bulls age significantly (P , 0.01) affected semen quality and sperm morphology. In adult bulls, semen volume, mass activity and sperm concentration were 36%, 17.56% and 19.6%, respectively, higher than young. Initial progressive motility (%) and livability showed significant (P , 0.01) improvement with the advancement of age (43.37 6 1.21 and 67.71 6 1.11, respectively, in young; 53.02 6 1.11 and 74.17 6 1.03, respectively, in adult). In young bulls, sperm head, mid piece, tail abnormality and total abnormal sperm percent (12.38 6 0.92, 4.87 6 0.24, 11.01 6 0.60 and 28.26 6 1.34, respectively) were 1.85, 1.27, 1.20 and 1.44 folds higher than that of their mature stage (6.69 6 0.64, 3.82 6 0.32, 9.14 6 0.64 and 19.66 6 1.31, respectively). Significant reduction (P , 0.01) in micro cephalic sperm, free heads, bent mid piece, looped mid piece and proximal protoplasmic droplets were observed at mature age as compared with their younger stage. In bulls of consistent freezing category, abnormal sperm heads significantly decreased from 4.40 6 0.31% to 3.28 6 0.02% on maturity. Similarly, in inconsistent freezing grade bulls sperm head abnormality (9.28 6 0.75% to 5.13 6 1.20%) and total abnormal sperm percent (24.89 6 1.43 to 18.73 6 3.40) was decreased over the age. On the contrary, in non-freezing category bulls' sperm morphology did not show significant (P . 0.05) improvement with age advancement, rather some abnormalities like long slender head, under developed/deformed head, abaxial implantation of mid piece, double mid piece, stump tail and distal protoplasmic droplets tend to increased significantly (P , 0.05) with age of bulls. Results indicated that in potential Frieswal bulls semen quality and sperm morphology were improved from young to mature stage, where as, in poor quality (non-freezing) semen producer bulls neither the morphology nor the semen quality showed any improvement with maturity. It was recommended that crossbred bulls producing more than 25% morphologically abnormal sperms in young age (below 30 months) along with poor progressive motility (,50%) and low sperm concentration (,1000 million/ml) need immediate culling with out any expectation of further improvement in semen quality with age advancement.Keywords: Sperm abnormality, Frieswal crossbred bull, Sperm morphology, Bos indicus 3 Bos taurus Implication Assessment of sperm morphology is one of the most important aspects for semen evaluation and successful fertilisation. High percentage of morphologically abnormal spe...

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Section: Effect Of Age On Bulls' Spermiogrammentioning

confidence: 77%

Effect of age on spermiogram of Holstein Friesian × Sahiwal crossbred bulls

Mandal

Kumar

Tyagi

2010

Animal

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“…In the boar, this is the major factor (70%) affecting cryosurvival , but this phenomenon is common in many species (bull (Chaveiro et al 2006), mouse (Songsasen & Leibo 1997) and dog (Yu et al 2002)) and influences whether donors can be used for cryopreservation. The reason for this significant male variation is not well understood but numerous causes, and potential markers, of freezing resistance have been investigated including regulation of cell volume (Chaveiro et al 2006), genetic factors (Thurston et al 2002), expression of housekeeping proteins (Casas et al 2009(Casas et al , 2010 and seminal plasma proteins (Jobim et al 2004, Asadpour et al 2007.…”

Section: Sperm Surface Alterations During Sperm Handlingmentioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

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Spermatozoa interact with their immediate environment and this contact remodels the sperm surface in preparation for fertilisation. These fundamental membrane changes will be critically covered in this review with special emphasis on the very specific surface destabilisation event, capacitation. This process involves very subtle and intricate modifications of the sperm membrane including removal of suppression (decapacitation) factors and changes in the lateral organisation of the proteins and lipids of the sperm surface. Processing of sperm for assisted reproduction (storage, sex-sorting, etc.) subjects spermatozoa to numerous stressors, and it is possible that this processing overrides such delicate processes resulting in sperm instability and cell damage. To improve sperm quality, novel mechanisms must be used to stabilise the sperm surface during handling. In this review, different types of membrane stress are considered, as well as novel surface manipulation methods to improve sperm stability.

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“…Seminal plasma proteins have been characterized by other investigators and their association with male fertility continues to be explored [2,3,[15][16][17]. Functions of sperm that may be affected by seminal plasma proteins include capacitation, acrosome reaction, motility, DNA integrity and interaction with the oocyte [3,18].…”

Section: Discussionmentioning

confidence: 99%

Characterization of proteins in cryopreserved and non-cryopreserved seminal plasma of dairy bulls of dif-fering fertility

Odhiambo¹,

Dailey²

2011

OJAS

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Seminal plasma is composed of secretions from accessory sex glands, which are mixed with sperm at ejaculation and contribute the majority of semen volume. Seminal plasma is considered a transport and support medium for sperm in the female reproductive tract. Because seminal plasma is not required for fertilization, the importance of its constituents to the establishment of normal pregnancy has been overlooked.

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Two-dimensional polyacrylamide gel electrophoresis of bovine seminal plasma proteins and their relation with semen freezability

Cited by 104 publications

References 48 publications

Effect of age on spermiogram of Holstein Friesian × Sahiwal crossbred bulls

Effect of age on spermiogram of Holstein Friesian × Sahiwal crossbred bulls

Sperm surface changes and physiological consequences induced by sperm handling and storage

Characterization of proteins in cryopreserved and non-cryopreserved seminal plasma of dairy bulls of dif-fering fertility

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