1995
DOI: 10.1002/yea.320110702
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Two‐Dimensional protein map of Saccharomyces cerevisiae: Construction of a gene–protein index

Abstract: This publication marks the beginning of the construction of a gene-protein index that relates proteins which are resolved on the two-dimensional protein map of Saccharomyces cerevisiae with their corresponding genes. We report the identification of 36 novel polypeptide spots on the yeast protein map. They correspond to the products of 26 genes. Together with the polypeptide spots previously identified, this raises to 41 the number of genes whose products have been identified on the protein map. The proteins id… Show more

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Cited by 81 publications
(73 citation statements)
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“…Proteins were extracted from lyophilized cells as described in [35]. Carrier ampholite isoelectrofocusing (CA-IEF) was performed in the pH range 4.5-7.…”
Section: Protein Extraction and First Dimensionmentioning
confidence: 99%
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“…Proteins were extracted from lyophilized cells as described in [35]. Carrier ampholite isoelectrofocusing (CA-IEF) was performed in the pH range 4.5-7.…”
Section: Protein Extraction and First Dimensionmentioning
confidence: 99%
“…Carrier ampholite isoelectrofocusing (CA-IEF) was performed in the pH range 4.5-7. Proteins were separated in gels (23.5 cm long, 1.3 mm diameter) containing 3.6% acrylamide (29.8%T, 4.7%C), 9.5 M urea, 3.6% CHAPS and 4% carrier ampholytes (2% Pharmalyte 5-8, 1% Pharmalyte 5-6, and 1% Pharmalyte 3-10, Amersham Biosciences, Uppsala, Sweden [35]). Gels were polymerized for 1.5 h by addition of 0.028% w/v ammonium persulfate (APS) and 0.028% TEMED.…”
Section: Protein Extraction and First Dimensionmentioning
confidence: 99%
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