Two Dimensional Quantitative Analysis in Planar Chromatography

Abstract

The quantitative analysis of biological materials presents serious problems for the biochemist. procedures to produce gels and separate phospholipids are time consuming and tedious. In the past, interpretation of the data was qualitative at best and quite subjective. sophisticated instrumentation and software, accurate and reliable data can be obtained. TheWith the introduction of In this paper, the methodology and techniques of two dimensional planar chromatography are explored. We will show that parameter selections can greatly influence the data. Comparisons of actual to calculated concentrations can show a 10% -55% variation depending on the sample, methodology and techniques used.