The characterization of scanning tip morphology is crucial for accurate linewidth measurements. Conventional rectangular characterizers are affected by lateral distortion caused by the nonlinearities in AFM scanning, leading to errors between the actual characterization results and the true values. In this study, we innovatively developed self-traceable two-dimensional nano-gratings using chromium atomic deposition technology and extreme ultraviolet interference lithography. We used this structure as a characterizer for conducting scanning tip characterizations.This paper analyzed the periodic stability of the grating sample during scanning and corrected the lateral distortion of atomic force microscopy (AFM) at scan scales of 0.5 µm and 1 µm based on its self-traceable characteristics. Additionally, we extracted the angle information of the scanning tip in the X direction and Y direction within a scan scale of 0.5 µm. The results demonstrate that the two-dimensional grating sample exhibited excellent periodic stability during scanning. The characterization errors for the tip’s X direction and Y direction angles are within ±2°, showing high consistency. This study highlights that self-traceable two-dimensional grating samples have the capability for in situ bidirectional characterization of tip information, providing a creative solution for the development of new-style tip characterizers.