Two‐Dimensional Visualization of Cholesterol and Cholesteryl Esters Within Human Coronary Plaques by Near‐Infrared Fluorescence Angioscopy

Background: High-density lipoprotein (HDL) plays a key role in reverse cholesterol transport, and halts the progression of atherosclerosis. The aim of the present study was to visualize native HDL in the human coronary arterial wall. Methods and Results:The fluorescence characteristics of HDL were investigated by color fluorescent microscopy (CFM) using excitation at 470 nm and emission at 515 nm with Fast green dye (FG) as the biomarker. HDL in 30 normal coronary segments, and in 25 white and 25 yellow plaques in excised human coronary arteries, was visualized by color fluorescent angioscopy (CFA) and CFM. Localization of HDL visualized by CFM was compared with that stained by immunostaining using an anti-HDL antibody. FG elicited a characteristic brown fluorescence of HDL. By CFA, the percent incidence of HDL in normal segments, white (early stage of plaque growth) and yellow (advanced stage of plaque growth) plaques was, respectively, 33%, 76% (P<0.05 vs. normal segments and yellow plaques) and 21%. Localization of HDL visualized by CFM did not differ from that stained by immunostaining. Conclusions:In the human coronary arterial wall, HDL deposits infrequently in normal segments, but increasingly deposits with plaque formation, and decreases in the advanced stage of plaque growth. (Circ J 2014; 78: 1667 -1675

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“…27, 28 CFA is now routinely used clinically to image coronary plaques. 11,29, 30 Although invasive, the present CFA system using …”

Section: Funding Sourcesmentioning

confidence: 98%

Imaging of Native High-Density Lipoprotein in Human Coronary Plaques by Color Fluorescent Angioscopy

Uchida

Hiruta

Yamanoi

et al. 2014

Circ J

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“…Our search for a biomarker of LDL that would be clinically applicable revealed indocyanine green dye (ICG) 10e12 elicits near-infrared fluorescence (NIRF) characteristic of human LDL only. Therefore, in the present study, molecular imaging of native LDL in human coronary plaques by an NIRF angioscopy (NIRFA) system 13,14 was performed using ICG as a biomarker of LDL ex vivo and in vivo.…”

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confidence: 99%

Molecular Imaging of Native Low-Density Lipoprotein by Near-Infrared Fluorescent Angioscopy in Human Coronary Plaques

Uchida¹,

Yoshida

Shimoyama

et al. 2016

The American Journal of Cardiology

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Low-density lipoprotein (LDL) is an important risk factor for coronary artery disease, but its localization within the human coronary arterial wall is poorly understood. Imaging of LDL in 30 coronary arteries excised from 15 subjects who underwent autopsy was performed using near-infrared fluorescent angioscopy system and using indocyanine green dye as a biomarker of LDL. The percentage incidence of LDL in 28 normal segments, 24 white plaques (early stage of plaque growth), and 21 yellow plaques (mature stage of plaque) classified by conventional angioscopy, was 14.2, 79.1 (p <0.01 vs normal segments and p <0.05 vs yellow plaques), and 28.5, respectively. Coronary near-infrared fluorescent angioscopy showed similar results in 7 patients in vivo. Our results suggested that LDL begins to deposit in the human coronary arterial wall in the early stage of atherosclerosis, increasingly deposits with plaque growth and decreases in the mature stage; and therefore, molecular therapy targeting LDL should be started before plaque maturation.

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“…In clinical situations, the spotty type was found in yellow plaques by conventional angioscopy, and the homogenous type was found not only in yellow plaques but also in white plaque and in apparently normal coronary segments [30].…”

Section: Imaging Of Lipids In the Plaques By Nir Fl Uorescence Angiosmentioning

confidence: 99%

Coronary angioscopy: From tissue imaging to molecular imaging

Uchida¹

2009

curr cardiovasc imaging rep

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Angioscopy enables macroscopic pathological diagnosis of cardiovascular diseases from the inside. This imaging modality has been mainly directed to observation of coronary artery diseases. This method is now clinically used for classifying coronary plaques and thrombi, understanding mechanisms of acute coronary syndromes, evaluating percutaneous coronary interventions, evaluating plaque-stabilizing effects of drugs, and recently, for characterizing vulnerable plaques and vulnerable patients and accordingly for prediction of acute coronary syndromes. Fluorescence angioscopy was recently established and is now clinically used for molecular imaging of coronary plaques. Molecular and cellular imaging by this new imaging technology will contribute to more objective evaluation of coronary artery diseases.

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Two‐Dimensional Visualization of Cholesterol and Cholesteryl Esters Within Human Coronary Plaques by Near‐Infrared Fluorescence Angioscopy

Cited by 13 publications

References 26 publications

Imaging of Native High-Density Lipoprotein in Human Coronary Plaques by Color Fluorescent Angioscopy

Imaging of Native High-Density Lipoprotein in Human Coronary Plaques by Color Fluorescent Angioscopy

Molecular Imaging of Native Low-Density Lipoprotein by Near-Infrared Fluorescent Angioscopy in Human Coronary Plaques

Coronary angioscopy: From tissue imaging to molecular imaging

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