2023
DOI: 10.1002/pro.4851
|View full text |Cite
|
Sign up to set email alerts
|

Two distinct mechanisms of flavoprotein spectral tuning revealed by low‐temperature and time‐dependent spectroscopy

Andrey Nikolaev,
Elena V. Tropina,
Kirill N. Boldyrev
et al.

Abstract: Flavins such as flavin mononucleotide or flavin adenine dinucleotide are bound by diverse proteins, yet have very similar spectra when in the oxidized state. Recently, we developed new variants of flavin‐binding protein CagFbFP exhibiting notable blue (Q148V) or red (I52V A85Q) shifts of fluorescence emission maxima. Here, we use time‐resolved and low‐temperature spectroscopy to show that whereas the chromophore environment is static in Q148V, an additional protein‐flavin hydrogen bond is formed upon photoexci… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

0
0
0

Publication Types

Select...

Relationship

0
0

Authors

Journals

citations
Cited by 0 publications
references
References 75 publications
0
0
0
Order By: Relevance

No citations

Set email alert for when this publication receives citations?