Two F‐box/WD‐repeat proteins Pop1 and Pop2 form hetero‐ and homo‐complexes together with cullin‐1 in the fission yeast SCF (Skp1‐Cullin‐1‐F‐box) ubiquitin ligase

Kominami, Kin‐ichiro; Ochotorena, Itziar; Toda, Takashi

doi:10.1046/j.1365-2443.1998.00225.x

Cited by 98 publications

(103 citation statements)

References 69 publications

(101 reference statements)

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“…6 Phosphorylation of Cdc18 by the cyclin-dependent kinase (CDK) Cdc2 during S phase leads to its ubiquitin-mediated degradation via the SCF Pop1/Pop2 ubiquitin ligase complex. [7][8][9] Cdt1 is degraded during S phase via the Cul4-Ddb1-Cdt2 ubiquitin ligase complex. 10 The overexpression of Cdc18 is sufficient to induce extensive DNA re-replication (with DNA levels ~4-fold higher than in wild type).…”

Section: The Prevention Of Dna Re-replication In Yeast and Metazoansmentioning

confidence: 99%

Control of the Cdc6 replication licensing factor in metazoa: The role of nuclear export and the CUL4 ubiquitin ligase

Kim¹,

Kipreos²

2008

Cell Cycle

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Section: The Prevention Of Dna Re-replication In Yeast and Metazoansmentioning

confidence: 99%

Control of the Cdc6 replication licensing factor in metazoa: The role of nuclear export and the CUL4 ubiquitin ligase

Kim¹,

Kipreos²

2008

Cell Cycle

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“…The Cdc4-related Pop1 and Pop2/Sud1 (19 -22) participate in the formation of SCF as either hetero-or homodimers (21)(22)(23). In pop mutants, both the short-lived Cdc2-Cdc13 inhibitor Rum1 and the S-phase regulator Cdc18 were stabilized (20,21). Rum1 is the functional homologue of Sic1 (24), and Cdc18 is the homologue of budding yeast Cdc6 (25).…”

mentioning

confidence: 99%

Skp1 and the F-box Protein Pof6 Are Essential for Cell Separation in Fission Yeast

Hermand¹,

Bamps²,

Tafforeau³

et al. 2003

Journal of Biological Chemistry

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Here we report functional characterization of the essential fission yeast Skp1 homologue. We have created a conditional allele of skp1 (skp1-3f) mimicking the mutation in the budding yeast skp1-3 allele. Although budding yeast skp1-3 arrests at the G 1 /S transition, skp1-3f cells progress through S phase and instead display two distinct phenotypes. A fraction of the skp1-3f cells arrest in mitosis with high Cdc2 activity. Other skp1-3f cells as well as the skp1-deleted cells accumulate abnormal thick septa leading to defects in cell separation. Subsequent identification of 16 fission yeast F-box proteins led to identification of the product of pof6 (for pombe F-box) as a Skp1-associated protein. Interestingly, cells deleted for the essential pof6 gene display a similar cell separation defect noted in skp1 mutants, and Pof6 localizes to septa and cell tips. Purification of Pof6 demonstrates association of Skp1, whereas the Pcu1 cullin was absent from the complex. These findings reveal an essential non-Skp1-Cdc53/Cullin-F-box protein function for the fission yeast Skp1 homologue and the F-box protein Pof6 in cell separation.

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“…As the interaction between Mcs2, Pmh1, and Skp1 did not reflect SCF-dependent regulation, we tested the capacity of Pcu1 [57], the scaffold protein in SCF, to interact with Mcs2 and Pmh1. Three strains were generated expressing both Pcu1 with a myc-tag at the C-terminus (Pcu1-13myc, a kind gift of Tanaka [58]) and TAP-tagged Mcs6, Mcs2, or Pmh1 at their respective endogenous loci.…”

Section: Fission Yeast Cak Regulation By Proteasomedependent Proteolysismentioning

confidence: 99%

Mcs2 and a novel CAK subunit Pmh1 associate with Skp1 in fission yeast

Bamps

Westerling

Pihlak

et al. 2004

Biochemical and Biophysical Research Communications

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The Mcs6 CDK together with its cognate cyclin Mcs2 represents the CDK-activating kinase (CAK) of fission yeast Cdc2. We have attempted to determine complexes in which Mcs6 and Mcs2 mediate this and possible other functions. Here we characterize a novel interaction between Mcs2 and Skp1, a component of the SCF (Skp1-Cullin-F box protein) ubiquitin ligase. Furthermore, we identify a novel protein termed Pmh1 through its association with Skp1. Pmh1 associates with the Mcs6-Mcs2 complex, enhancing its kinase activity, and represents the apparent homolog of metazoan Mat1. Association of Mcs2 or Pmh1 with Skp1 does not appear to be involved in proteolytic degradation, as these complexes do not contain Pcu1, and levels of Mcs2 or Pmh1 are not sensitive to inhibition of SCF and the 26S proteasome. The identified interactions between Skp1 and two regulatory CAK subunits may reflect a novel mechanism to modulate activity and specificity of the Mcs6 kinase.

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Two F‐box/WD‐repeat proteins Pop1 and Pop2 form hetero‐ and homo‐complexes together with cullin‐1 in the fission yeast SCF (Skp1‐Cullin‐1‐F‐box) ubiquitin ligase

Cited by 98 publications

References 69 publications

Control of the Cdc6 replication licensing factor in metazoa: The role of nuclear export and the CUL4 ubiquitin ligase

Control of the Cdc6 replication licensing factor in metazoa: The role of nuclear export and the CUL4 ubiquitin ligase

Skp1 and the F-box Protein Pof6 Are Essential for Cell Separation in Fission Yeast

Mcs2 and a novel CAK subunit Pmh1 associate with Skp1 in fission yeast

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