Two Forms of the Nuclear Matrix–Bound p53 Protein in HEK293 Cells

Lapshina, M. A.; Parkhomenko, I. I.; Terentiev, Alexei A.

doi:10.1196/annals.1378.019

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…6 There is evidence to support that in vivo studies involving colon cancer cells, an initial Cav-1 downregulation need not be an entirely irreversible process since cell survival on selection for increased metastatic potential normally in these cell lines. [21][22][23] For example, the p53 present in MCF-7 cell lines is weak and usually unresponsive, whereas in ZR75-1 breast cancer cell line and in murine in vivo analysis, DNA-damaging drugs such as doxorubicin are unable to stabilize the p53 present, suggesting normal p53 activity is impaired. 24 The absence of Cav-1 expression in these non-metastatic but impaired p53-possesing breast cell lines may reflect much like the p53 heterozygous or p53-null cell lines (shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Divergent control of Cav-1 expression in non-cancerous Li-Fraumeni syndrome and human cancer cell lines

Sherif

Sultan

2013

Cancer Biology & Therapy

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Li-Fraumeni syndrome (LFS) is primarily characterized by development of tumors exhibiting germ-line mutations in the p53 gene. Cell lines developed from patients of a LFS family have decreased p53 activity as evidenced by the absence of apoptosis upon etoposide treatment. To test our hypothesis that changes in gene expression beyond p53 per se are contributing to the development of tumors, we compared gene expression in non-cancerous skin fibroblasts of LFS-affected (p53 heterozygous) vs. non-affected (p53 wild-type homozygous) family members. Expression analysis showed that several genes were differentially regulated in the p53 homozygous and heterozygous cell lines. We were particularly intrigued by the decreased expression (~88%) of a putative tumor-suppressor protein, caveolin-1 (Cav-1), in the p53-mutant cells. Decreased expression of Cav-1 was also seen in both p53-knockout and p21-knockout HTC116 cells suggesting that p53 controls Cav-1 expression through p21 and leading to the speculation that p53, Cav-1 and p21 may be part of a positive auto-regulatory feedback loop. The direct relationship between p53 and Cav-1 was also tested with HeLa cells (containing inactive p53), which expressed a significantly lower Cav-1 protein. A panel of nonfunctional and p53-deficient colon and epithelial breast cancer cell lines showed undetectable expression of Cav-1 supporting the role of p53 in the control of Cav-1. However, in two aggressively metastasizing breast cancer cell lines, Cav-1 was strongly expressed suggesting a possible role in tumor metastasis. Thus, there is a divergent control of Cav-1 expression as evidenced in non-cancerous Li-Fraumeni syndrome and some aggressive human cancer cell lines.

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Section: Discussionmentioning

confidence: 99%

Divergent control of Cav-1 expression in non-cancerous Li-Fraumeni syndrome and human cancer cell lines

Sherif

Sultan

2013

Cancer Biology & Therapy

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“…Cell nuclei were purified as described elsewhere [7]. The cells were lysed in 0.25 STC buffer (0.25 M sucrose, 50 mM tris-HCl pH 9.0, 5 mM CaCl 2 , 20 mM NH 4 Cl, 2 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol) containing 0.2% Triton X-100.…”

Section: Methodsmentioning

confidence: 99%

Lamin B Content in Chromatin Fractions after Purification of Nuclear Matrix from Cells of Different Types

et al. 2008

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We analyzed the content of lamin B, one of the main proteins of the nuclear membrane, in different chromatin fractions obtained during purification of the nuclear matrix from different cell types. Depending on cell type and nuclear matrix preparation technique, lamin B was found in different not associated with matrix chromatin compartments. This effect was observed after chromatin extraction with ammonium chloride after nucleolysis and after chromatin extraction with sodium chloride before nuclease treatment. These findings suggest that the structure of the nuclear matrix is destabilized in certain extraction procedures and that studies of subcompartmentalization of nuclear macromolecules require additional control of nuclear matrix integrity.

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Two Forms of the Nuclear Matrix–Bound p53 Protein in HEK293 Cells

Cited by 2 publications

References 5 publications

Divergent control of Cav-1 expression in non-cancerous Li-Fraumeni syndrome and human cancer cell lines

Divergent control of Cav-1 expression in non-cancerous Li-Fraumeni syndrome and human cancer cell lines

Lamin B Content in Chromatin Fractions after Purification of Nuclear Matrix from Cells of Different Types

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