Two‐Fragment‐Dummy‐Template Molecularly Imprinted Polymers Mn Doped ZnS Quantum Dots Based Room‐Temperature Phosphorescene Probing for Hepatotoxic Homologues of Microcystin

Abstract

Fragment-dummy-template molecularly imprinted polymers-Mn doped ZnS QDs (FMIPÀ Mn doped ZnS QDs) were synthesized by surface imprinting technique and specifically recognize microcystin (MC), especially MC-LR, MC-RR and MC-YR. Pentylbenzene and L-arginine were used as fragment dummy template of MC to form specific recognition site cavity. The introduction of dummy template reduced the toxicity and cost of MC as template in the synthesis process. When MC was added to the FMIP, the room temperature phosphorescence (RTP) intensity is quenched regularly, thereby achieving the effect of detecting MC. The two-fragment-dummy-template molecularly imprinted polymers-Mn doped ZnS QDs (FMIP-1) have distinct linearly RTP quenching toward MC in the concentration range of 0.0025-0.0175 nM. The linear relationship between ΔP/P 0 and C MC-LR , C MC-YR and C MC-RR were observed with the regression equation ΔP/P 0 = 0.0150 + 24.70C MC-LR , ΔP/ P 0 = 0.0995 + 21.38C MC-YR , and ΔP/P 0 = 0.101 + 16.96C MC-RR (where concentration was in nM), with the detection limit of 0.25 pM, 1 pM and 0.6 pM, respectively. This method was applied to detect MC in water and freshwater products. The method have the potential for effective detection of other harmful substances in the complicated matrix.

A dummy molecularly imprinted ratiometric fluorescence nanosensor for the sensitive detection of guanidyl-microcystins in environmental water

“…Mbukwa et al 19 successfully fabricated MIPs using L-arginine for imprinting with a dummy template and the MIPs finally showed good performance in the determination of MCs. Li et al 20 proposed a dual-fragment dummy template imprinting strategy based on Mn-ZnS quantum dots combined with room temperature phosphorescence to detect MCs homologues. Furthermore, the MIPbased ratiometric fluorescence sensing detection has attracted significant attention [21][22][23][24][25] because of its high sensitivity, fast response, facile sample pretreatment, and anti-interference.…”