Two genes for mitochondrial tyrosine transfer RNA in yeast

Wésolowski, M; Palleschi, Claudio; Gostinelli, Manuela; Frontali, Laura; Fukuhara, Hiroshi

doi:10.1016/0014-5793(81)80713-2

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…Figure 1 shows typical chromatographic patterns of mitochondrial tyrosyl tRNA species from two strains, T and M. These strains are genetically established laboratory stocks. A major difference between the two chromatograms is the absence of species 1 in strain M. Another strain, F, with the same mitochondrial DNA as M but a different nuclear genotype, also showed a pattern similar to M. Whereas the other major species, 2, 4, and 5, are all products of a single gene, tyrA, species 1 is transcribed from a second gene, tyrB (6). Strains M and F, which lack species 1, were found, nevertheless, to possess the tyrB gene, since it could be detected by the hybridization of 3H-tyrosyl tRNA species 1 at the defined position on the mitochondrial DNA.…”

Section: Methodsmentioning

confidence: 96%

Role of Nuclear Genes in Expression of a Mitochondrial tRNA Gene in Saccharomyces cerevisiae

Wésolowski

Palleschi²,

Frontali³

et al. 1983

Molecular and Cellular Biology

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In yeast mitochondria, most of the isoaccepting species of tyrosyl tRNA are coded by a mitochondrial gene, tyrA. A particular isoaccepting species is coded by a second mitochondrial gene, tyrB. This gene is not expressed in certain strains of yeast which show no deficient phenotype. Genetic crosses between strains expressing or not expressing the tyrB gene demonstrate that expression is controlled by specific nuclear genes and that a mutation of the tyrA gene can be bypassed when the tyrB gene is operative.

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Section: Methodsmentioning

confidence: 96%

Role of Nuclear Genes in Expression of a Mitochondrial tRNA Gene in Saccharomyces cerevisiae

Wésolowski

Palleschi²,

Frontali³

et al. 1983

Molecular and Cellular Biology

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“…The yeast strains used in this study are listed in Table 1 (6). Strains M and F, which lack species 1, were found, nevertheless, to possess the tyrB gene, since it could be detected by the hybridization of 3H-tyrosyl tRNA species 1 at the defined position on the mitochondrial DNA.…”

Section: Methodsmentioning

confidence: 99%

“…Species 1, however, is a product of a different mitochondrial gene called tyrB. This gene maps far from the known tyrA gene (6). Interestingly, species 1 tRNA is not detectable in some laboratory strains despite the presence of the tyrB gene.…”

mentioning

confidence: 99%

Role of nuclear genes in expression of a mitochondrial tRNA gene in Saccharomyces cerevisiae.

Wesolowski¹,

Palleschi²,

Frontali³

et al. 1983

Mol. Cell. Biol.

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(5). Cytoduction by means of Kar-mutation. To study the expression of a given mitochondrial genome in the presence of different nuclear genomes, we applied the technique of Kar-cytoduction. The technique was introduced by Conde and Fink (3) and was studied in detail by Lancashire and Matoon (4) for use in mitochondrial genetics. The strain carrying the mitochondrial genome to be studied is first crossed to a [rho] karl-i strain of opposite mating type. The mutation karl-i is a dominant nuclear mutation resulting in defective nuclear fusion in mating cells. The abortive cross gives rise to haploid cells which carry the mitochondrial DNA of interest and the karl-i nucleus.These cells are in turn crossed to a [rho"] derivative of a strain with a desired nuclear genotype. Again, an abortive cross occurs, and the haploid cells carrying the original mitochondrial DNA and the desired nucleus are selected. The nuclear genotype is identified by the presence of specific auxotrophic markers.

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