Two GEO MicroRNA Expression Profile Based High-Throughput Screen to Identify MicroRNA-31-3p Regulating Growth of Medullary Thyroid Carcinoma Cell by Targeting RASA2

Jiang, Mei; Shi, Xin; Zhu, Hua; Wu, Wei; Li, Jinyan

doi:10.12659/msm.916815

Cited by 9 publications

(8 citation statements)

References 45 publications

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“…[ 11 ] In addition, miRNA-3065-5p was found to be downregulated in melanoma. [ 13 ] Jiang et al confirmed that miRNA-31-3p is downregulated in medullary thyroid carcinoma (MTC), [ 14 ] which is contrast to the present finding. In addition, it was also demonstrated that miRNA-31-3p inhibited MTC cell proliferation by negatively regulating the RASA2 expression.…”

Section: Discussioncontrasting

confidence: 84%

MicroRNA expression profiling and target gene analysis in gastric cancer

Xie

Liu

et al. 2020

Medicine

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This study aims to identify differentially expressed microRNAs (miRNAs) in gastric cancer by comparing gastric cancerous tissues with normal tissues, explore the potential roles. The miRNA expression microarray was employed on gastric cancer tissues, and apparently normal para-cancerous tissues from 3 patients undergoing radical surgery were matched. Quantitative RT-PCR was performed on the other 7 patients to validate the findings of the microarray. Furthermore, Gene Ontology (GO) analysis and enrichment analysis of KEGG Pathway were performed for 5 dysregulated candidate miRNAs, including 3 upregulated (miR-31-3p, miR-6736-3p, and miR-147b) and 2 downregulated (miR-3065-5p and miR-3921) miRNAs, in order to determine the role of miRNAs in tumorigenesis and development. Among these miRNAs, 17 miRNAs were found to be upregulated, and 19 miRNAs were found to be downregulated. The dysregulated expression of 5 candidate miRNAs, including miR-31-3p, miR-147b, miR-6736-3p, miR-3065-5p, and miR-3921, were verified by quantitative RT-PCR in the validation set. Among these miRNAs, miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921 had 551 target gene intersections. The GO and KEGG Pathway analyses Revealed that miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921 may participate in multiple pathophysiological processes, such as foreign substance metabolism and chemical carcinogenesis. The profile of differentially expressed miRNAs was successfully screened, and 4 miRNAs (i.e., miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921) appeared to be involved in gastric carcinogenesis. These might serve as promising biomarkers for gastric cancer.

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Section: Discussioncontrasting

confidence: 84%

MicroRNA expression profiling and target gene analysis in gastric cancer

Xie

Liu

et al. 2020

Medicine

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“…Two human MTC cell lines (TT, MZ-CRC-1), and one normal human thyrocyte cell line (NThyy-ori 3.1) were used for the study as TT and MZ-CRC-1 are the two of most widely used MTC-derived cell lines [ 23 – 25 ]. They were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

circPVT1 regulates medullary thyroid cancer growth and metastasis by targeting miR-455-5p to activate CXCL12/CXCR4 signaling

Zheng

Shu

Wang

et al. 2021

J Exp Clin Cancer Res

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Background Medullary thyroid cancer (MTC) represents 13.4 % of all thyroid cancers-related deaths. The treatments for MTC are very limited especially for patients with distal metastasis. Therefore, it is critical to understand the mechanisms of MTC to pursue novel therapeutic avenues. Here, we studied the function of circPVT1/miR-455-5p in MTC. Methods Human MTC tissues and cell lines were used. qRT-PCR and Western blotting were employed to measure expression levels of miR-455-5p, circPVT1, CXCL12, and epithelial mesenchymal transformation (EMT)-related proteins. Colony formation assay, flow cytometry, transwell assay, and scratch wound healing assay were used to assess the abilities of cell proliferation, apoptosis, migration and invasion, respectively. Dual luciferase assay and RNA immunoprecipitation were employed to validate interactions of circPVT1/miR-455-5p and miR-455-5p/CXCL12. Nude mouse xenograft model was used to evaluate the effects of shcircPVT1 and miR-455-5p mimics on tumor growth and metastasis in vivo. Results miR-455-5p was reduced in MTC tissues and cells while circPVT1 was elevated. Their levels were correlated with prognosis of MTC. Overexpression of miR-455-5p or sh-circPVT1 suppressed EMT and MTC cell proliferation, migration and invasion. miR-455-5p targeted CXCL12 while circPVT1 sponged miR-455-5p. Knockdown of CXCL12 or CXCL12/CXCR4 signaling inhibitor reversed the effects of circPVT1 overexpression or miR-455-5p inhibitor on EMT and MTC cell proliferation, migration and invasion. Knockdown of circPVT1 or miR-455-5p overexpression repressed MTC tumor growth and lung metastasis in vivo. Conclusions miR-455-5p suppresses MTC growth and metastasis by targeting CXCL12/CXCR4 signaling pathway while circPVT1 promotes MTC by sponging miR-455-5p. Our study sheds light on the mechanisms of MTC growth and metastasis.

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“…RAS gene mutation abrogates its switching capacity leaving it in a constitutively active state, promoting diseased conditions ( 38 , 39 ). It has been shown that the Ras signaling pathway plays significant roles in cellular differentiation, proliferation, apoptosis, and carcinogenesis ( 38 – 40 ). In addition, Ras can also activate the MAPK and PI3K/AKT pathways, leading to the progression of thyroid cancer ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

Construction of a Signature Model to Predict the Radioactive Iodine Response of Papillary Thyroid Cancer

et al. 2022

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Papillary thyroid cancer (PTC) accounts for about 90% of thyroid cancer. There are approximately 20%–30% of PTC patients showing disease persistence/recurrence and resistance to radioactive iodine (RAI) treatment. For these PTC patients with RAI refractoriness, the prognosis is poor. In this study, we aimed to establish a comprehensive prognostic model covering multiple signatures to increase the predictive accuracy for progression-free survival (PFS) of PTC patients with RAI treatment. The expression profiles of mRNAs and miRNAs as well as the clinical information of PTC patients were extracted from TCGA and GEO databases. A series of bioinformatics methods were successfully applied to filtrate a two-RNA model (IPCEF1 and hsa-mir-486-5p) associated with the prognosis of RAI-therapy. Finally, the RNA-based risk score was calculated based on the Cox coefficient of the individual RNA, which achieved good performances by the time-dependent receiver operating characteristic (tROC) curve and PFS analyses. Furthermore, the predictive power of the nomogram, integrated with the risk score and clinical parameters (age at diagnosis and tumor stage), was assessed by tROC curves. Collectively, our study demonstrated high precision in predicting the RAI response of PTC patients.

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Two GEO MicroRNA Expression Profile Based High-Throughput Screen to Identify MicroRNA-31-3p Regulating Growth of Medullary Thyroid Carcinoma Cell by Targeting RASA2

Cited by 9 publications

References 45 publications

MicroRNA expression profiling and target gene analysis in gastric cancer

MicroRNA expression profiling and target gene analysis in gastric cancer

circPVT1 regulates medullary thyroid cancer growth and metastasis by targeting miR-455-5p to activate CXCL12/CXCR4 signaling

Construction of a Signature Model to Predict the Radioactive Iodine Response of Papillary Thyroid Cancer

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