Two highly sensitive and selective coumarin-based fluorometric probes for the detection of ClO⁻ and cell imaging

Abstract

Two new coumarin-based fluorescent probes were synthesized and successfully applied for the efficient detection of ClO− as turn-on fluorescence chemosensors by the oxidative cleavage of C–O and CN, the detection limits (LOD) for the two probes are 154 nM and 32 nM, respectively.

A Fluorescent Probe with a Significant Selective Turn‐On Response for HClO Detection and Bioimaging in Living Cells

“…With the increase of ClO − , the emission intensity of CCO at 530 nm was continuously and regularly enhanced. The fluorescence enhancement mechanism may be due to the redox reaction of the surface lactone groups of CCO . A more detailed information is found that the emission response of probe CCO to ClO − had a good linear relationship (R 2 =0.996) in the range of 0–8.5 μ M (Figure S4) .…”

Fluorescent Probes for HOCl Detection in Living Cells

“…In 2019, Jin and coauthors proposed two probes of the switch and turn-on types with a coumarin core, BCO and BETC, respectively (Fig. 7) [62]. Switching BCO (λ ex = 372 nm; λ em = 430/460 nm) and turning or C=O and C=N bonds (in the case of BETC) under the effect of HOCl with the formation of a common end product (NOA, Fig.…”

In situ fabricated MOF–cellulose composite as an advanced ROS deactivator-convertor: fluoroswitchable bi-phasic tweezers for free chlorine detoxification and size-exclusive catalytic insertion of aqueous H2O2

“…2 However, its uncontrolled production can cause cardiological diseases, cancer and tissue damage, 3,4 while hypochlorite residue in water gets converted into highly toxic constituents. 5,6 On a similar note, H 2 O 2 is also a reactive oxygen generator that serves as a signaling agent in cellular activities, helps in the removal of organic impurities from wastewater, 2,7–10 and acts as a common preservative. Nevertheless, excessive accumulation of H 2 O 2 results in intracellular oxidative stress and can cause pathological defects that lead to fatal diseases like cancer, genetic instability and diabetes.…”