Two Homologs of the FLOWERING LOCUS C Gene from Leaf Mustard (Brassica juncea)

Martynov, V. V.; Хавкин, Э. Е.

doi:10.1023/b:rupp.0000019220.43439.c9

Cited by 7 publications

(9 citation statements)

References 21 publications

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“…The major gene in the Arabidopsis photoperiod pathway is the CONSTANS gene, while in the autonomous and vernalization pathways the major gene is FLC (Simpson et al 1999). Research progress in Arabidopsis has enabled the identification and cloning of related genes in Brassica that are known to directly influence the onset of flowering (Lagercrantz et al 2002;Martynov andKhavkin 2004, Osborn andLukens 2003). In Brassica, CONSTANS has been proposed to have the greatest influence in the flowering time variation in B. nigra and B. oleracea (Bouhon et al 1998;Lagercrantz et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Characterization of flowering time and SSR marker analysis of spring and winter type Brassica napus L. germplasm

et al. 2006

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Flowering dates and life forms of all available Brassica napus accessions conserved at the North Central Regional Plant Introduction Station (NCRPIS) were characterized, and a survey of molecular variation was conducted by using simple sequence repeats (SSR) in order to support better management of accessions with diverse life forms. To characterize flowering phenology, 598 B. napus accessions from the NCRPIS collection were planted in Iowa and Kansas field sites together with a current commercial cultivar and observed for days to flowering (first, 50% and 100% flowering) in 2003. Days from planting to 50% flowering ranged from 34 to 83 in Iowa and from 53 to 89 in Kansas. The mean accumulated growing degree days (GDD) to 50% flowering were 1,997 in Iowa, and 2,106 in Kansas. Between locations, the correlation in flowering time (r = 0.42) and the correlation in computed GDD (r = 0.40) were both significant. Differences in flowering-time rank were observed for several accessions. Accessions that failed to flower in Iowa in a single growing season comprised 28.5% of the accessions; of the flowering accessions, 100% plant flowering was not always achieved. Accessions were grouped according to flowering time. A stratified sample of 50 accessions was selected from these groups, including 10 non-flowering and 40 flowering accessions of diverse geographic origins and phenological variation. The flowering time observed in the sampled accessions when grown in the greenhouse were found to be significantly correlated to the flowering time observed in the field locations in Iowa (r = 0.79) and Kansas (r = 0.49). Thirty SSR markers, selected across 18 Brassica linkage groups from BrassicaDB, and 3 derived from Brassica expressed sequence tags (ESTs) were scored in the stratified sample. An average of three bands per SSR primer pair was observed.Associations of SSR marker fragments with the life forms were determined. Analysis of molecular variation by using cluster analysis and ordination resulted in recognizable, distinct groups of annual and biennial life-form types, which may have direct applications for planning and management of future seed regenerations.

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Section: Discussionmentioning

confidence: 99%

Characterization of flowering time and SSR marker analysis of spring and winter type Brassica napus L. germplasm

et al. 2006

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“…1). The alignment of nucleotide sequences of the most variable exon 4 of the arabidopsis FLC3 with all currently known structural homologs of this gene [16] makes obvious that the probe used in the present study would hybridize both to homologs and paralogs of FLC; in this way the number of possible hybridization zones would considerably increase.…”

Section: Discussionmentioning

confidence: 99%

“…This comparison showed that the FLC sequences were clustered by the physiological function of the FLC homologs, that is, by their association with a particular chromosomal locus in control of time to flowering, rather than by their species specificity [16]. In the present study, we used another method of assessing the polymorphism of FLC homologs in The methods for isolating genomic DNA and RFLP analysis were described in previous communications [16,19]. For DNA digestion, 2.5 units of endonuclease MspI (Fermentas, Lithuania) were added per 1 µg DNA.…”

Section: Introductionmentioning

confidence: 99%

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RFLP Analysis of the FLOWERING LOCUS C Gene in Six Brassica Species

Martynov

Хавкин

2005

Russ J Plant Physiol

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The FLOWERING LOCUS C (FLC) gene controls the transition of arabidopsis plants to flowering following cold induction (vernalization). Time to flowering in annual and biennial species of Brassicaceae supposedly depends on the number of FLC copies. We analyzed DNA restriction fragment length polymorphism in six Brassica species with diploid (AA, BB, and CC) and allotetraploid (AABB, AACC, and BBCC) genomes using for a hybridization probe an FLC homolog previously cloned in our laboratory from B. juncea. The characteristic variations in the patterns of restriction fragments corresponded to the genomic composition of Brassica species and, in some cases, correlated with the timing of floral transition.

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“…In the present study, we compared the diversity of the coding sequences within the CONSTANS family to their restriction site polymorphisms and found a distinct divergence in plant forms derived from the subtropical and temperate regions, which probably mirrored plant photoperiodic responses. The methods for isolating genomic DNA, amplifying genome fragments using PCR, cloning the amplified genome fragments, and determining their nucleotide and derived amino acid sequences were described earlier [15]. The degenerate primers (1) TCATGT-GAGCGWGCCCC and (2) TGAAGCATACCT-TATYGTCTTCTC were used to amplify CONSTANS homologs; these primers recognize highly conserved stretches corresponding to zinc finger and CCT domains in arabidopsis, oilseed rape, and black mustard (Fig.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of the CONSTANS gene in Brassica plants

Martynov

Хавкин

2005

Russ J Plant Physiol

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Using a direct amplification of genomic DNA from two Brassica rapa forms, we obtained two homologs of the CONSTANS gene, which controls the photoperiodic induction of flowering in Arabidopsis plants. The cloned fragments of B. rapa genome were identified as members of the CONSTANS-LIKE1 class. By aligning the nucleotide sequences of the CONSTANS gene and its homologs, three classes, CONSTANS, CONSTANS-LIKE1 , and CONSTANS-LIKE2 , were distinctly discerned by their primary structure. The pattern of restriction fragment length polymorphisms (RFLP) of the CONSTANS homologs in B. carinata, B. juncea, B. napus, B. nigra, B. oleracea , and B. rapa were genome-specific; in addition, the CONSTANS homologs were classified by plant geographic origin, and we assume that such classification is related to plant photoperiodic response.

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Two Homologs of the FLOWERING LOCUS C Gene from Leaf Mustard (Brassica juncea)

Cited by 7 publications

References 21 publications

Characterization of flowering time and SSR marker analysis of spring and winter type Brassica napus L. germplasm

Characterization of flowering time and SSR marker analysis of spring and winter type Brassica napus L. germplasm

RFLP Analysis of the FLOWERING LOCUS C Gene in Six Brassica Species

Polymorphism of the CONSTANS gene in Brassica plants

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