Two human monoclonal SARS-CoV-2 antibodies that maintain neutralizing potency against the SARS-CoV-2 Omicron BA.1 and BA.2 variants

Qin, Zheng; Duan, Liangwei; Jiang, Zhihua; Gu, Tingxuan; Zhang, Bojie; Li, Jiaoyang; Zhang, Yang; Zhang, Shiyu; Liang, Yingming; Wang, Hui

doi:10.1016/j.gendis.2022.05.027

Cited by 2 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RBD protein acts as the main area of action for neutralizing antibodies induced during virus infection or following vaccination [ 21 , 22 ], which play a vital role in protecting severe COVID-19 and infection. Therefore, it is a component of almost all vaccine antigens.…”

Section: Resultsmentioning

confidence: 99%

“…Currently, it has been found that the SARS-CoV-2 receptor binding domain (RBD) subunit vaccine has been found could facilitate adapted immunity against virus infection [ 16 – 18 ]. RBD protein, which serves as an essential role in virus attachment and a major target for host immune responses, plays an important role in recognizing angiotensin-converting enzyme 2 (ACE2) and contributing to fusion and viral entry into the host cell [ 19 – 22 ]. Therefore, RBD protein has been an ideal antigen for numerous vaccine candidates.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activation and induction of antigen-specific T follicular helper cells play a critical role in recombinant SARS-CoV-2 RBD vaccine-induced humoral responses

Yang,

Duan,

Wang

et al. 2023

Mol Biomed

Self Cite

View full text Add to dashboard Cite

The role of follicular T helper (Tfh) cells in humoral response has been considered essential in recent years. Understanding how Tfh cells control complex humoral immunity is critical to developing strategies to improve the efficacy of vaccines against SARS-CoV-2 and other emerging pathogens. However, the immunologic mechanism of Tfh cells in SARS-CoV-2 receptor binding domain (RBD) vaccine strategy is limited. In this study, we expressed and purified recombinant SARS-CoV-2 RBD protein in Drosophila S2 cells for the first time and explored the mechanism of Tfh cells induced by RBD vaccine in humoral immune response. We mapped the dynamic of Tfh cell in lymph node and spleen following RBD vaccination and revealed the relationship between Tfh cells and humoral immune response induced by SARS-CoV-2 RBD vaccine through correlation analysis, blocking of IL-21 signaling pathway, and co-culture of Tfh with memory B cells. Recombinant RBD protein elicited a predominant Tfh1 and Tfh1-17 subset response and strong GC responses in spleen and lymph nodes, especially to enhanced vaccination. IL-21 secreted by Tfh cells affected the development and differentiation of B cells and played a key role in the humoral immune response. These observations will help us further understand the mechanism of protective immune response induced by COVID-19 vaccine and has guiding significance for the development of vaccines against newly emerging mutants.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Activation and induction of antigen-specific T follicular helper cells play a critical role in recombinant SARS-CoV-2 RBD vaccine-induced humoral responses

Yang,

Duan,

Wang

et al. 2023

Mol Biomed

Self Cite

View full text Add to dashboard Cite

show abstract

“…CoronaVac that was a β-propyl lactone-inactivated SARS-CoV-2 vaccine developed by Sinovac Life Sciences in China and generated protective efficacy up to 79.34% [ 8 – 10 ]. Most previously published reports on CoronaVac vaccine have focused on vaccine-induced neutralizing antibidies [ 11 – 13 ]. However, the adaptive immune system is important for control of most viral infections.…”

Section: Introductionmentioning

confidence: 99%

Adaptive immune responses and cytokine immune profiles in humans following prime and boost vaccination with the SARS-CoV-2 CoronaVac vaccine

Wang

Yang

Duan

et al. 2022

Virol J

Self Cite

View full text Add to dashboard Cite

Background Adaptive immune response has been thought to play a key role in SARS-CoV-2 infection. The role of B cells, CD4+T, and CD8+T cells are different in vaccine-induced immune response, thus it is imperative to explore the functions and kinetics of adaptive immune response. We collected blood samples from unvaccinated and vaccinated individuals. To assess the mechanisms contributing to protective immunity of CoronaVac vaccines, we mapped the kinetics and durability of humoral and cellular immune responses after primary and boost vaccination with CoronaVac vaccine in different timepoints. Materials and methods We separate PBMC and plasma from blood samples. The differentiation and function of RBD-spcific CD4+T and CD8+T cells were analyzed by flow cytometry and ELISA. Antibodies response was analyzed by ELISA. ELISPOT analysis was perfomed to detected the RBD-spcific memory B cells. CBA analysis was performed to detected the cytokine immune profiles. Graphpad prism 8 and Origin 2021 were used for statistical analysis. Results Vaccine-induced CD4+T cell responses to RBD were more prominent than CD8+T cell responses, and characterized by a predominant Th1 and weak Th17 helper response. CoronaVac vaccine triggered predominant IgG1 antibody response and effectively recalled specific antibodies to RBD protein after booster vaccination. Robust antigen-specific memory B cells were detected (p < 0.0001) following booster vaccination and maintained at 6 months (p < 0.0001) following primary vaccination. Vaccine-induced CD4+T cells correlated with CD8+T cells (r = 0.7147, 0.3258, p < 0.0001, p = 0.04), memory B cell responses (r = 0.7083, p < 0.0001), and IgG and IgA (r = 0.6168, 0.5519, p = 0.0006, 0.003) after vaccination. In addition, vaccine induced a broader and complex cytokine pattern in plasma at early stage. Conclusion Taken together, these results highlight the potential role of B cell and T cell responses in vaccine-induced long-term immunity.

show abstract

Two human monoclonal SARS-CoV-2 antibodies that maintain neutralizing potency against the SARS-CoV-2 Omicron BA.1 and BA.2 variants

Cited by 2 publications

References 5 publications

Activation and induction of antigen-specific T follicular helper cells play a critical role in recombinant SARS-CoV-2 RBD vaccine-induced humoral responses

Activation and induction of antigen-specific T follicular helper cells play a critical role in recombinant SARS-CoV-2 RBD vaccine-induced humoral responses

Adaptive immune responses and cytokine immune profiles in humans following prime and boost vaccination with the SARS-CoV-2 CoronaVac vaccine

Contact Info

Product

Resources

About