Two Hybrid Histidine Kinases Involved in the Ethylene Regulation of the Mycelial Growth and Postharvest Fruiting Body Maturation and Senescence of Agaricus bisporus

Zhang, Chaohui; Shang, Di; Zhang, Yan; Gao, Xiaodan; Liu, Dehai; Gao, Yuqian; Li, Yanan; Qi, Yuancheng; Qiu, Liyou

doi:10.1128/spectrum.02411-22

Cited by 6 publications

(6 citation statements)

References 76 publications

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“…Briefly: a 5.5 mL headspace vial contained a 1.8 mL mixture composed of 150 mM NaHCO 3 , 30 mM ascorbic acid, 1.0 mM ACC, 0.1 mM FeSO 4 , and 0.1 mg enzyme protein in 100 mM Tris-HCl buffer at pH 7.2. After shaking incubation at 30 °C for 1 h, 1 mL gas was withdrawn for ethylene determination by gas chromatography (GC-2010 plus, Shimadzu, Kyoto, Japan) [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

Phylogenesis of the Functional 1-Aminocyclopropane-1-Carboxylate Oxidase of Fungi and Plants

Mei

Zhang

et al. 2022

JoF

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The 1-aminocyclopropane-1-carboxylic acid (ACC) pathway that synthesizes ethylene is shared in seed plants, fungi and probably other organisms. However, the evolutionary relationship of the key enzyme ACC oxidase (ACO) in the pathway among organisms remains unknown. Herein, we cloned, expressed and characterized five ACOs from the straw mushroom (Volvariella volvacea) and the oyster mushroom (Pleurotus ostreatus): VvACO1-4 and PoACO. The five mushroom ACOs and the previously identified AbACO of the button mushroom contained all three conserved residues that bound to Fe(II) in plant ACOs. They also had variable residues that were conserved and bound to ascorbate and bicarbonate in plant ACOs and harbored only 1–2 of the five conserved ACO motifs in plant ACOs. Particularly, VvACO2 and AbACO had only one ACO motif 2. Additionally, VvACO4 shared 44.23% sequence identity with the cyanobacterium Hapalosiphon putative functional ACO. Phylogenetic analysis showed that the functional ACOs of monocotyledonous and dicotyledonous plants co-occurred in Type I, Type II and Type III, while putative functional gymnosperm ACOs also appeared in Type III. The putative functional bacterial ACO, functional fungi and slime mold ACOs were clustered in ancestral Type IV. These results indicate that ACO motif 2, ACC and Fe(II) are essential for ACO activity. The ACOs of the other organisms may come from the horizontal transfer of fungal ACOs, which were found ordinarily in basidiomycetes. It is mostly the first case for the horizontal gene transfers from fungi to seed plants. The horizontal transfer of ACOs from fungi to plants probably facilitates the fungal-plant symbioses, plant–land colonization and further evolution to form seeds.

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Section: Methodsmentioning

confidence: 99%

Phylogenesis of the Functional 1-Aminocyclopropane-1-Carboxylate Oxidase of Fungi and Plants

Mei

Zhang

et al. 2022

JoF

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“…Moreover, Western blotting results showed that LeHOG1 phosphorylation and LeATG8 protein levels followed the same trend. Now, ROS and the autophagy mechanism and their functions in relation to mushroom growth and fruit-body formation are becoming hot research topics [ 10 , 35 , 36 ]. Thus, the correlation between ROS and autophagy in L. edodes , as well as the role of LeHOG1 protein in ROS-mediated oxidative stress, require further study.…”

Section: Discussionmentioning

confidence: 99%

Insights into the Global Transcriptome Response of Lentinula edodes Mycelia during Aging

Gao

Fan

Wei

et al. 2023

JoF

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The spawn of Lentinula edodes and other basidiomycete fungi tend to age with long-term culture. This causes heavy yield losses if aging spawn is used for propagation. In this study, we cultivated dikaryotic L. edodes mycelia in plates for 60 days to produce intrinsic aging phenotypes. We found that intracellular reactive oxygen species levels increased in contrast to mitochondrial depolarization and also observed greater DNA fragmentation with longer culture time. Transcriptome analysis of mycelia at different growth stages revealed pronounced expression differences between short- and long-term cultures. In particular, “phenylalanine, tyrosine, and tryptophan biosynthesis”, “mitophagy and autophagy”, “MAPK signaling pathway”, and “ABC transporter” were among the enriched terms in the mycelial aging process. Weighted correlation network analysis identified LeAtg8, LeHog1, LePbs2, and LemTOR as key genes during aging. Western blotting confirmed that LeATG8 and phosphorylated LeHOG1 protein levels were significantly upregulated in aging mycelia. Our combined analytical approach provides insights into the mechanisms that regulate mycelial aging, indicating that autophagy/mitophagy plays a major role in counteracting the effects of age on mycelial growth development.

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“…In Cordyceps militaris , the best reference genes for inducing fruiting bodies in wheat medium and pupae are gpd / rpb1 and rpb1 / try , respectively [ 32 ]. However, during the fruiting bodies development of Flammulina velutipes , Botrytis cinerea and Agaricus bisporus , the stable reference genes are gapdh , tubulin and EF1-α , respectively [ 33 – 35 ]. In the research related to the development of O .…”

Section: Discussionmentioning

confidence: 99%

Stable reference gene selection for Ophiocordyceps sinensis gene expression studies under different developmental stages and light-induced conditions

Tong

Wei

Mao³

et al. 2023

PLoS ONE

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The molecular mechanism of Chinese cordyceps formation has received a substantial amount of attention because of its usage as traditional Chinese medicine. The formation process of Chinese cordyceps includes two parts: asexual proliferation (Ophiocordyceps sinensis proliferates in the hemolymph of Thitarodes armoricanus larvae) and sexual development (formation and development of fruiting bodies). Therefore, validation of reference genes under different development stages and experimental conditions is crucial for RT-qPCR analysis. However, there is no report on stable reference genes at the development stage of O. sinensis fruiting body. In this study, 10 candidate reference genes, Actin, Cox5, Tef1, Ubi, 18s, Gpd, Rpb1, Try, Tub1 and Tub2, were selected and calculated their expression stability using four methods: geNorm, NormFinder, BestKeeper, and Comparative △Ct. After comprehensive analysis of the results of these four methods with RefFinder, we determined that the most stable reference genes during asexual reproduction of O. sinensis were Tef1 and Tub1, while the most stable reference genes during fruiting body development were Tyr and Cox5, and the most stable reference genes under light-induced conditions were Tyr and Tef1. Our study provides a guidance for reference genes selections at different proliferation processes with light stress of O. sinensis, and represents a foundation for studying the molecular mechanism of Chinese cordyceps formation.

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Two Hybrid Histidine Kinases Involved in the Ethylene Regulation of the Mycelial Growth and Postharvest Fruiting Body Maturation and Senescence of Agaricus bisporus

Cited by 6 publications

References 76 publications

Phylogenesis of the Functional 1-Aminocyclopropane-1-Carboxylate Oxidase of Fungi and Plants

Phylogenesis of the Functional 1-Aminocyclopropane-1-Carboxylate Oxidase of Fungi and Plants

Insights into the Global Transcriptome Response of Lentinula edodes Mycelia during Aging

Stable reference gene selection for Ophiocordyceps sinensis gene expression studies under different developmental stages and light-induced conditions

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