Two Major Facilitator Superfamily Sugar Transporters from Trichoderma reesei and Their Roles in Induction of Cellulase Biosynthesis

Zhang, Weixin; Kou, Yanbo; Xu, Jintao; Cao, Yanli; Zhao, Guolei; Shao, Jing; Wang, Hai; Wang, Zhixing; Bao, Xinhe; Chen, Guanjun; Liu, Weifeng

doi:10.1074/jbc.m113.505826

Cited by 140 publications

(226 citation statements)

References 34 publications

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“…We also found that the function of three of the top scoring transporters had been studied previously (Huang et al, 2015;Ramos et al, 2006;Saloheimo et al, 2007;Zhang et al, 2013). One of them, TrStr1 (protID 50894) (Huang et al, 2015) was confirmed to function as xylose transporter, while Stp1 (protID 47710) and TrHxt1 (protID 22912) were associated to cellobiose and glucose utilization respectively (Ramos et al, 2006;Zhang et al, 2013).…”

Section: A Niger and T Reesei In Silico Proteome Miningsupporting

confidence: 67%

“…In this regard, the A. niger analyzing the T. reesei transcriptomic response to wheat straw, many of the HMM xylT top scoring transporters were upregulated on this carbon source versus glucose (Bischof et al, 2013;Ries et al, 2013). We also found that the function of three of the top scoring transporters had been studied previously (Huang et al, 2015;Ramos et al, 2006;Saloheimo et al, 2007;Zhang et al, 2013). One of them, TrStr1 (protID 50894) (Huang et al, 2015) was confirmed to function as xylose transporter, while Stp1 (protID 47710) and TrHxt1 (protID 22912) were associated to cellobiose and glucose utilization respectively (Ramos et al, 2006;Zhang et al, 2013).…”

Section: A Niger and T Reesei In Silico Proteome Miningsupporting

confidence: 55%

“…The biochemical characteristics of the six studied transporters, plus those reported previously (Sloothaak et al, 2015;Vankuyk PA et al, 2004;Zhang et al, 2013), revealed the wide range of glucose and xylose uptake systems featured by these fungi. The initial uptake kinetics of both sugars displayed by XltA and XltB suggested a possible biological role for these transporters in xylose utilization.…”

Section: Analysis Of Uptake Kinetics Bymentioning

confidence: 99%

“…Several transporters from cluster 4 were previously studied, showing the relevance of this subgroup for sugar uptake (Sloothaak et al, 2015;Vankuyk PA et al, 2004;Zhang et al, 2013). Taking this into account, and the fact that MstA has been shown to have high affinity towards xylose, we decided to select another T. reesei representative of this cluster (TrStr3, ProtID 62380) for further studies.…”

Section: Phylogenetic Clustering Of Top Scoring a Niger And T Reesementioning

confidence: 99%

“…Fumarate can be found as an intermediate in various different metabolic subsystems, and a number of pathways have been investigated for fumarate production in several microbial cell-factories such as Saccharomyces cerevisiae, Torulopsis glabrata, Scheffersomyces stipitis and Escherichia coli (Chen et al, , 2016Li et al, 2014;Song et al, 2013;Xu et al, 2013Xu et al, , 2012aZhang et al, 2015). Despite the large number of pathways leading to fumarate, the current consensus is that the reductive route of the TCA cycle in the cytosol is responsible for fumarate accumulation in R. delemar (Kenealy et al, 1986;Osmani and Scrutton, 1985;Overman and Romano, 1969;Peleg et al, 1989;Romano et al, 1967).…”

Section: Introductionmentioning

confidence: 99%

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Organic acid production in Aspergillus niger and other filamentous fungi

Odoni

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Propositions1. Medium acidification is primarily a cause, rather than an effect, of Aspergillus niger citrate secretion.(this thesis) 2. A holistic systems biology approach should consider the organism in the context of its environment.(this thesis) 3. Metabolism has more than two spacial dimensions and should be studied as a system rather than a map.4. Cancer cells behave as a microorganism in a foreign environment.5. Referrals to authors rather than researchers highlights the skewed perception of scientific worth.6. Reviews can both progress and regress a field.7. Recent history suggests that "common sense" is an oxymoron.8. True understanding, and thus friendship, arises from a common mindset rather than a common nationality.Propositions belonging to the thesis, entitled

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Section: A Niger and T Reesei In Silico Proteome Miningsupporting

confidence: 67%

Section: A Niger and T Reesei In Silico Proteome Miningsupporting

confidence: 55%

Section: Analysis Of Uptake Kinetics Bymentioning

confidence: 99%

Section: Phylogenetic Clustering Of Top Scoring a Niger And T Reesementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Organic acid production in Aspergillus niger and other filamentous fungi

Odoni

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Enhancing peptaibols production in the biocontrol fungus Trichoderma longibrachiatum SMF2 by elimination of a putative glucose sensor

Zhou

Song

et al. 2019

Biotech & Bioengineering

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Trichoderma spp. are main producers of peptide antibiotics known as peptaibols. While peptaibols have been shown to possess a range of biological activities, molecular understanding of the regulation of their production is largely unclear, which hampers the production improvement through genetic engineering. Here, we demonstrated that the orthologue of glucose sensors in the outstanding biocontrol fungus Trichoderma longibrachiatum SMF2, TlSTP1, participates in the regulation of peptaibols production. Deletion of Tlstp1 markedly impaired hyphal growth and conidiation, but significantly increased peptaibols yield by 5‐fold for Trichokonins A and 2.6‐fold for Trichokonins B. Quantitative real‐time polymerase chain reaction analyses showed that the increased peptaibols production occurs at the transcriptional levels of the two nonribosomal peptide synthetase encoding genes, tlx1 and tlx2. Transcriptome analyses of the wild type and the Tlstp1 mutant strains indicated that TlSTP1 exerts a regulatory effect on a set of genes that are involved in a number of metabolic and cellular processes, including synthesis of several other secondary metabolites. These results suggest an important role of TlSTP1 in the regulation of vegetative growth and peptaibols production in T. longibrachiatum SMF2 and provide insights into construction of peptaibol‐hyperproducing strains through genetic engineering.

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