Two major RNA products are transcribed from heat-shock locus 93D ofDrosophila melanogaster

Ryseck, Rolf Peter; Walldorf, Uwe; Hovemann, Bernd

doi:10.1007/bf01259440

Cited by 25 publications

(15 citation statements)

References 9 publications

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“…The levels of hsr transcripts are specifically regulated at different embryonic and larval stages [99]. Embryonic stages show fluctuating hsr mRNA levels, while second-stage larvae exhibit low transcript levels.…”

Section: Reviewsmentioning

confidence: 99%

Regulation of heat shock gene induction and expression during Drosophila development

Michaud¹,

Marín²,

Tanguay³

1997

CMLS, Cell. mol. life sci.

110

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Some heat shock genes are expressed in the absence of stress during embryogenesis and metamorphosis in the fruit fly Drosophila melanogaster. Their functions in these processes are unknown. During development, each of the four members of the small heat shock protein family (Hsp27, Hsp26 Hsp23 and Hsp22), which are coordinately induced in response to a heat stress, shows a specific pattern of expression in diverse tissues and cells. This expression is driven through cell-specific enhancers in the promoter regions of their genes. In addition, some of the Hsps show cell-specific induction by heat shock. Hsp23, for example, is only inducible in a single cell type (cone cells) of the eye ommatidium, while the other small Hsps are inducible in all cells of the eye unit. In germ line tissues such as testes, Hsp23 and 27 are both readily expressed in the absence of stress (albeit in distinct cell lineages) and cannot be further induced by heat shock. Hsp27 is expressed throughout oogenesis, but its intracellular localization is stage-specific, being nuclear from germarium to stage 6 and cytoplasmic from stage 8 onwards. Finally the small Hsps show tissue-specific post-translational modifications. Thus the function(s) of the small Hsps may be modulated by different cell and developmental stage-specific mechanisms operating either on their expression, their cellular localization or their structure by post-translational modifications.

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Section: Reviewsmentioning

confidence: 99%

Regulation of heat shock gene induction and expression during Drosophila development

Michaud¹,

Marín²,

Tanguay³

1997

CMLS, Cell. mol. life sci.

110

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“…The hsrω gene is reported to produce a set of three transcripts (Ryseck et al 1985;Hovemann et al 1986). The largest nucleus-limited hsrω-n transcript is >10 kb and corresponds to the entire transcription unit composed of the 5′ proximal unique region encompassing two exons and an intron followed by a distal stretch of tandem repeats extending for 5 to~15 kb at the 3′ end.…”

Section: Introductionmentioning

confidence: 99%

Pleiotropic consequences of misexpression of the developmentally active and stress-inducible non-coding hsrω gene in Drosophila

Mallik

Lakhotia

2011

J Biosci

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The non-coding hsrω gene of Drosophila melanogaster is expressed in nearly all cell types and developmental stages. However, in the absence of conventional mutant alleles of this gene, its developmental functions remain largely unknown. In the present study, we used a variety of GAL4 drivers to overexpress or ablate this gene’s transcripts in specific tissues and examined the developmental consequences thereof. Our results show that a balanced expression of these non-coding transcripts is critical for survival and normal development in all the tissue types tested, since any change in cellular levels of these transcripts in a given cell type generally has detrimental effects, with extreme cases resulting in organismal lethality, although in a few cases the misexpression of these transcripts also suppresses the mutant phenotype due to other genetic conditions. Evidence is also presented for existence of a new spliced variant of the hsrω-n nuclear transcript. Following the RNAi-mediated down-regulation of hsrω transcripts, the omega speckles disappear so that the nucleoplasmic hnRNPs get diffusely distributed, while upregulation of these transcripts results in greater sequestration of these proteins into omega speckle clusters; either of these conditions would affect activities of the hnRNPs and other hsrω-RNA interacting proteins, which is likely to have cascading consequences. The present findings, together with our earlier observations on effects of altered levels of the hsrω transcripts on induced apoptosis and expanded polyQ-mediated neurodegeneration, further confirm that ncRNA species like the hsrω, far from being evolutionary hangovers, provide critical information for important functions in normal cells.

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“…2D), and locus 33B was not scored. The heat shock puffs that are associated with PEP do not strictly correspond to those heat shock genes that are also induced by ecdysone, such as the small heat shock protein genes at locus 67B (Zimmerman et al 1983) or with heat shock genes containing introns, the hsp83 gene at locus 63BC (Yost and Lindquist 1986), and the hsr93D gene at locus 93D (Ryseck et al 1985;Garbe and Pardue 1986). Thus, PEP is not required for the processes of puffing, transcription, or RNA processing per se.…”

Section: Pep Is Associated Preferentially With Active Ecdysone-inducmentioning

confidence: 99%

A unique zinc finger protein is associated preferentially with active ecdysone-responsive loci in Drosophila.

Amero¹,

Elgin²,

Beyer³

1991

Genes Dev.

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Using an immunochemical approach, we have identified a unique antigen, PEP (protein on ecdysone puffs), which is associated in third-instar larvae with the active ecdysone-regulated loci on polytene chromosomes; PEP is not associated with most intermolt puffs and is found on some, but not all, heat shock-induced puffs. The distribution pattern changes with changing puffing patterns in the developmental program. We have screened an expression library and recovered a cDNA clone encoding PEP. PEP possesses multiple potential nucleic acid-and protein-binding regions: a glycine-and asparagine-rich amino terminus, four zinc finger motifs, two very acidic segments, two short basic stretches, and an alanine-and proline-rich carboxyl terminus. The Pep gene maps by in situ hybridization to the cytological locus 74F, adjacent to the early ecdysone-responsive region; however, the gene is not regulated by ecdysone at the level of transcription. The pattern of Pep expression through development suggests that maternal Pep gene transcripts are supplied to the embryo, and that the abundance of Pep gene transcripts decreases to a lower, fairly constant level thereafter. This unusual protein may play a role in the process of gene activation, or possibly in RNA processing, for a defined set of developmentally regulated loci.

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Two major RNA products are transcribed from heat-shock locus 93D ofDrosophila melanogaster

Cited by 25 publications

References 9 publications

Regulation of heat shock gene induction and expression during Drosophila development

Regulation of heat shock gene induction and expression during Drosophila development

Pleiotropic consequences of misexpression of the developmentally active and stress-inducible non-coding hsrω gene in Drosophila

A unique zinc finger protein is associated preferentially with active ecdysone-responsive loci in Drosophila.

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