Two methods for the assessment of the oxygen content of small volumes of seawater

Peck, Lloyd S.; Uglow, R. F.

doi:10.1016/0022-0981(90)90157-8

Cited by 55 publications

(28 citation statements)

References 10 publications

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“…Respirometers were maintained at stable temperatures by placement in flowing seawater at ambient temperatures during respirometry measurements. At the end of the measurement period, oxygen concentrations in the respirometers were measured using couloximetric techniques (Peck & Uglow 1990, Peck & Whitehouse 1992. At the same time, water samples were collected for measurement of ammonia, urea and primary amines.…”

Section: Methodsmentioning

confidence: 99%

Feast and famine in Antarctica: seasonal physiology in the limpet Nacella concinna

Fraser¹,

Clarke²,

Peck³

2002

Mar. Ecol. Prog. Ser.

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) in winter. Urea and primary amine excretion combined accounted for between 10 and 38% of total nitrogen excretion. O:N ratios ranged between 7 and 20 and demonstrated that although protein was the dominant respiratory substrate throughout the year, in late summer and early winter lipids and carbohydrates were also utilised for metabolism. N. concinna has a low degree of metabolic seasonality due to the maintenance of high winter metabolic rates. Although feeding during winter provides some energy, it is insufficient to offset the substantial utilisation of tissue energy stores to balance the metabolic budget. KEY WORDS: Limpet · O:N ratio · Nitrogen excretion · Oxygen consumption · Seasonal metabolismResale or republication not permitted without written consent of the publisher

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Section: Methodsmentioning

confidence: 99%

Feast and famine in Antarctica: seasonal physiology in the limpet Nacella concinna

Fraser¹,

Clarke²,

Peck³

2002

Mar. Ecol. Prog. Ser.

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“…Oxygen consumption of the bivalves was calculated by comparison with parallel incubations of identical control chambers with no animal. The oxygen content of the water in chambers was measured using 25-mL samples injected into a couloximeter (Peck and Uglow 1990). The syringe was fitted with a Chaney adaptor and was calibrated weekly from samples ( ) weighed on a mi-N p 10 crobalance.…”

Section: Oxygen Consumption and Hemolymph Oxygen Measuresmentioning

confidence: 99%

Metabolic Demand, Oxygen Supply, and Critical Temperatures in the Antarctic BivalveLaternula elliptica

Peck

Pörtner²,

Hardewig³

2002

Physiological and Biochemical Zoology

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150

116

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Oxygen consumption ( ), heartbeat rate and form, and cirMo 2 culating hemolymph oxygen content were measured in relation to temperature in the large Antarctic infaunal bivalve Laternula elliptica. After elevations in temperature from 0Њ to 3Њ, 6Њ, and then 9ЊC, and heartbeat rate rose to new levels, whereaṡ Mo 2 maximum circulating hemolymph oxygen content fell. At 0ЊC, was 19.6 mmol O 2 h Ϫ1 for a standard animal of 2-g tissuė Mo 2 ash-free dry mass, which equates to a 8.95-g tissue dry-mass or 58.4-g tissue wet-mass animal. Elevation of metabolism following temperature change had acute Q 10 values between 4.1 and 5, whereas acclimated figures declined from 3.4 (between 0Њ and 3ЊC) to 2.2 (3Њ-6ЊC) and 1.9 (6Њ-9ЊC). Heartbeat rate showed no acclimation following temperature elevations, with Q 10 values of 3.9, 3.2, and 4.3, respectively. Circulating hemolymph oxygen content declined from 0Њ to 3Њ and 6ЊC but stayed at a constant Po 2 (73-78 mmHg) and constant proportion (∼50%) of the oxygen content of the ambient water. At 9ЊC, and heartbeat rate both peaked at values 3.3 times thosė Mo 2 measured at 0ЊC, which may indicate aerobic scope in this species. After these peaks, both measures declined rapidly over the ensuing 5 d to the lowest measured in the study, and the bivalves began to die. Hemolymph oxygen content fell dramatically at 9ЊC to values between 2% and 12% of ambient water O 2 content and had a maximum Po 2 of around 20 mmHg. These data indicate an experimental upper lethal temperature of 9ЊC and a critical temperature, where a long-term switch to

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“…Periods of this duration have previously been shown to be necessary for oxygen consumption (V O 2 ) in L. uva to reach basal levels (Peck 1989). The respiration rates of the brachiopods were then measured using closed bottle methods and a couloximeter to assess oxygen content of respirometers, as previously described (Peck & Uglow 1990;Peck & Whitehouse 1992;Peck 1996).…”

Section: (B) Respiration Rate Assessmentsmentioning

confidence: 99%

Growth and metabolism in the Antarctic brachiopodLiothyrella uva

Peck

Brockington

Brey

1997

Phil. Trans. R. Soc. Lond. B

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107

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Summer and winter growth rates were assessed separately for a population of the Antarctic brachiopod Liothyrella uva between early January 1992 and December 1993. Annual shell growth rates (1.6–2.3 mm yr −1 for a 5 mm individual; 0.96–1.44 mm −1 for a 20 mm specimen) were two to six times slower than those reported for temperate species. Growth in specimens less than 20 mm in length was faster in 1992 than in 1993, although differences between years over the whole size range were not significant. Surprisingly, growth was much faster in winter periods than during the summers. A 5 mm long individual grew five times faster in winter than in summer, and for a 20 mm long specimen the difference was 13 times. This runs contrary to current ideas on the effects of seasonality on the biology of polar marine invertebrates, but may be an effect of maximizing the efficiency of resource utilization. Comparisons with previous work showed shell growth to be decoupled from periods of tissue mass increase, and also from the main period of phytoplankton productivity. Oxygen consumption of 75 of the specimens used in the growth study was measured to test the hypothesis that basal metabolic rates should be inversely correlated with growth rates. Unexpectedly, an analysis of residuals produced no significant relationship, positive or negative, between growth rate and basal metabolism ( F = 1.37, p =0.25, n = 75).

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Two methods for the assessment of the oxygen content of small volumes of seawater

Cited by 55 publications

References 10 publications

Feast and famine in Antarctica: seasonal physiology in the limpet Nacella concinna

Feast and famine in Antarctica: seasonal physiology in the limpet Nacella concinna

Metabolic Demand, Oxygen Supply, and Critical Temperatures in the Antarctic BivalveLaternula elliptica

Growth and metabolism in the Antarctic brachiopodLiothyrella uva

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